TACI (TNFRSF13B) (NM_012452) Human Tagged ORF Clone

CAT#: RC211856

TNFRSF13B (Myc-DDK-tagged)-Human tumor necrosis factor receptor superfamily, member 13B (TNFRSF13B)

Product Images

Western blot validation of overexpression lysate (Cat# LY402216) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211856 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TNFRSF13B
• Synonyms: CD267; CVID; CVID2; IGAD2; RYZN; TACI; TNFRSF14B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211856 representing NM_012452
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCCTGGGCCGGAGCAGGCGAGGTGGCCGGAGCCGTGTGGACCAGGAGGAGCGCTTTCCACAGG
  GCCTGTGGACAGGGGTGGCTATGAGATCCTGCCCCGAAGAGCAGTACTGGGATCCTCTGCTGGGTACCTG
  CATGTCCTGCAAAACCATTTGCAACCATCAGAGCCAGCGCACCTGTGCAGCCTTCTGCAGGTCACTCAGC
  TGCCGCAAGGAGCAAGGCAAGTTCTATGACCATCTCCTGAGGGACTGCATCAGCTGTGCCTCCATCTGTG
  GACAGCACCCTAAGCAATGTGCATACTTCTGTGAGAACAAGCTCAGGAGCCCAGTGAACCTTCCACCAGA
  GCTCAGGAGACAGCGGAGTGGAGAAGTTGAAAACAATTCAGACAACTCGGGAAGGTACCAAGGATTGGAG
  CACAGAGGCTCAGAAGCAAGTCCAGCTCTCCCGGGGCTGAAGCTGAGTGCAGATCAGGTGGCCCTGGTCT
  ACAGCACGCTGGGGCTCTGCCTGTGTGCCGTCCTCTGCTGCTTCCTGGTGGCGGTGGCCTGCTTCCTCAA
  GAAGAGGGGGGATCCCTGCTCCTGCCAGCCCCGCTCAAGGCCCCGTCAAAGTCCGGCCAAGTCTTCCCAG
  GATCACGCGATGGAAGCCGGCAGCCCTGTGAGCACATCCCCCGAGCCAGTGGAGACCTGCAGCTTCTGCT
  TCCCTGAGTGCAGGGCGCCCACGCAGGAGAGCGCAGTCACGCCTGGGACCCCCGACCCCACTTGTGCTGG
  AAGGTGGGGGTGCCACACCAGGACCACAGTCCTGCAGCCTTGCCCACACATCCCAGACAGCGGCCTTGGC
  ATTGTGTGTGTGCCTGCCCAGGAGGGGGGCCCAGGTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211856 representing NM_012452
  Red=Cloning site Green=Tags(s)
  
  MSGLGRSRRGGRSRVDQEERFPQGLWTGVAMRSCPEEQYWDPLLGTCMSCKTICNHQSQRTCAAFCRSLS
  CRKEQGKFYDHLLRDCISCASICGQHPKQCAYFCENKLRSPVNLPPELRRQRSGEVENNSDNSGRYQGLE
  HRGSEASPALPGLKLSADQVALVYSTLGLCLCAVLCCFLVAVACFLKKRGDPCSCQPRSRPRQSPAKSSQ
  DHAMEAGSPVSTSPEPVETCSFCFPECRAPTQESAVTPGTPDPTCAGRWGCHTRTTVLQPCPHIPDSGLG
  IVCVPAQEGGPGA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012452
• ORF Size: 879 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012452.1, NM_012452.2, NP_036584.1
• RefSeq Size: 1377 bp
• RefSeq ORF: 882 bp
• Locus ID: 23495
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Primary immunodeficiency
• MW: 31.6 kDa
• Gene Summary: The protein encoded by this gene is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It interacts with calcium-modulator and cyclophilin ligand (CAML). The protein induces activation of the transcription factors NFAT, AP1, and NF-kappa-B and plays a crucial role in humoral immunity by interacting with a TNF ligand. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]