OR52B4 (NM_001005161) Human Tagged ORF Clone

CAT#: RC212134

OR52B4 (Myc-DDK-tagged)-Human olfactory receptor, family 52, subfamily B, member 4 (OR52B4)

Product Images

Western blot validation of overexpression lysate (Cat# LY423948) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212134 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR52B4
• Synonyms: OR11-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212134 representing NM_001005161
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTACTGTAAACCACAGTGGCACTAGCCACACAGTCTTCCACTTGCTGGGCATCCCTGGCCTACAGG
  ACCAGCACATGTGGATTTCTATCCCATTCTTCATTTCCTATGTCACCGCCCTTCTTGGGAACAGCCTGCT
  CATCTTCATTATCCTCACAAAGCGCAGCCTCCATGAACCCATGTACCTCTTCCTCTGCATGCTGGCTGGA
  GCAGACATTGTCCTCTCCACGTGCACCATTCCTCAGGCCTTAGCTATCTTCTGGTTCCGTGCTGGGGACA
  TCTCCCTGGATCGTTGCATCACTCAGCTCTTCTTCATCCATTCCACCTTCATCTCTGAGTCAGGGATCTT
  GCTGGTGATGGCCTTTGACCACTATATTGCCATATGCTACCCACTGAGGTACACCACCATTCTTACAAAT
  GCTCTGATCAAGAAAATTTGTGTGACTGTCTCTCTGAGAAGTTATGGTACAATTTTCCCTATCATATTTC
  TTTTAAAAAGATTGACTTTCTGCCAGAATAATATTATTCCACACACCTTTTGTGAACACATTGGCCTAGC
  CAAATATGCATGTAATGACATTCGAATAAACATTTGGTATGGGTTTTCCATTCTAATGTCGACGGTGGTC
  TTAGATGTTGTACTAATTTTTATTTCCTATATGCTGATTCTCCATGCTGTCTTCCACATGCCTTCTCCAG
  ATGCTTGCCACAAAGCTCTCAACACATTTGGCTCCCATGTCTGCATCATCATCCTCTTTTATGGGTCTGG
  CATCTTCACAATCCTTACCCAGAGGTTTGGACGCCACATTCCACCTTGTATCCACATCCCGTTGGCTAAT
  GTCTGCATTCTGGCTCCACCTATGCTGAATCCCATTATTTATGGGATCAAAACCAAGCAAATCCAGGAAC
  AGGTGGTTCAGTTTTTGTTTATAAAACAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212134 representing NM_001005161
  Red=Cloning site Green=Tags(s)
  
  MPTVNHSGTSHTVFHLLGIPGLQDQHMWISIPFFISYVTALLGNSLLIFIILTKRSLHEPMYLFLCMLAG
  ADIVLSTCTIPQALAIFWFRAGDISLDRCITQLFFIHSTFISESGILLVMAFDHYIAICYPLRYTTILTN
  ALIKKICVTVSLRSYGTIFPIIFLLKRLTFCQNNIIPHTFCEHIGLAKYACNDIRINIWYGFSILMSTVV
  LDVVLIFISYMLILHAVFHMPSPDACHKALNTFGSHVCIIILFYGSGIFTILTQRFGRHIPPCIHIPLAN
  VCILAPPMLNPIIYGIKTKQIQEQVVQFLFIKQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005161
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005161.1, NM_001005161.2, NM_001005161.3, NP_001005161.1, NP_001005161.2
• RefSeq Size: 945 bp
• RefSeq ORF: 945 bp
• Locus ID: 143496
• Cytogenetics: 11p15.4
• Protein Families: Druggable Genome
• Protein Pathways: Olfactory transduction
• MW: 35.4 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. This olfactory receptor gene is a segregating pseudogene, where some individuals have an allele that encodes a functional olfactory receptor, while other individuals have an allele encoding a protein that is predicted to be non-functional. [provided by RefSeq, Jun 2015]