SYT homolog 1 (SS18L1) (NM_198935) Human Tagged ORF Clone

CAT#: RC212373

SS18L1 (Myc-DDK-tagged)-Human synovial sarcoma translocation gene on chromosome 18-like 1 (SS18L1)

Product Images

Western blot validation of overexpression lysate (Cat# LY404653) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212373 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SS18L1
• Synonyms: CREST; LP2261
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212373 representing NM_198935
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGTGGCCTTCGCGTCTGCCCGGCCAAGAGGCAAAGGGGAGGTTACGCAGCAAACCATCCAGAAGA
  TGCTGGACGAGAACCACCACCTGATCCAGTGCATCCTGGAGTACCAGAGCAAGGGCAAGACGGCCGAGTG
  CACGCAGTACCAGCAGATCCTGCACCGGAACCTGGTATACCTGGCCACGATCGCAGACTCCAACCAGAAC
  ATGCAGTCCCTGCTTCCTGCCCCGCCCACGCAGAACATGAACCTGGGCCCTGGAGCCCTGACTCAGAGCG
  GCTCCAGCCAGGGCCTGCACTCTCAGGGCAGCCTGAGTGACGCCATCAGCACGGGCCTGCCACCCTCCTC
  CCTCCTGCAGGGCCAGATTGGCAACGGGCCGAGCCACGTGTCCATGCAGCAGACGGCGCCTAACACGCTG
  CCCACCACCTCCATGAGCATCTCTGGGCCCGGCTACAGCCACGCGGGACCCGCCTCGCAGGGCGTCCCCA
  TGCAGGGGCAAGGCACCATCGGCAACTACGTGTCTCGGACCAACATCAACATGCAGTCCAACCCAGTCTC
  CATGATACAGCAGCAGGCGGCCACGTCGCACTACAGCTCGGCGCAGGGCGGCAGCCAGCACTACCAGGGC
  CAGTCGTCCATCGCCATGATGGGGCAGGGCAGCCAGGGGAGCAGCATGATGGGGCAGCGGCCCATGGCGC
  CCTACCGGCCCTCCCAGCAAGGCTCTTCCCAGCAGTACCTGGGCCAGGAGGAGTACTATGGCGAGCAGTA
  CAGCCACAGCCAGGGCGCCGCGGAGCCCATGGGCCAGCAGTACTACCCCGACGGCCATGGCGATTACGCC
  TACCAGCAGTCATCCTACACGGAGCAGAGCTACGACCGGTCCTTCGAGGAGTCCACGCAGCACTACTATG
  AGGGGGGAAACTCCCAGTACAGCCAGCAGCAGGCCGGGTACCAGCAGGGTGCCGCGCAGCAGCAGACGTA
  CTCCCAGCAGCAGTACCCCAGCCAGCAGAGCTACCCCGGGCAGCAGCAGGGCTACGGGTCTGCCCAGGGA
  GCCCCGTCACAGTACCCCGGCTACCAGCAAGGCCAAGGCCAGCAGTACGGAAGCTACCGAGCACCGCAGA
  CAGCGCCGTCTGCCCAGCAGCAGCGGCCCTACGGCTATGAACAGGGCCAGTATGGAAATTACCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212373 representing NM_198935
  Red=Cloning site Green=Tags(s)
  
  MSVAFASARPRGKGEVTQQTIQKMLDENHHLIQCILEYQSKGKTAECTQYQQILHRNLVYLATIADSNQN
  MQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQGSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTL
  PTTSMSISGPGYSHAGPASQGVPMQGQGTIGNYVSRTNINMQSNPVSMIQQQAATSHYSSAQGGSQHYQG
  QSSIAMMGQGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGHGDYA
  YQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPSQQSYPGQQQGYGSAQG
  APSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYGNYQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198935
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_198935.1, NM_198935.2, NP_945173.1
• RefSeq Size: 4566
• RefSeq ORF: 1191
• Locus ID: 26039
• MW: 42.8 kDa
• Gene Summary: This gene encodes a calcium-responsive transactivator which is an essential subunit of a neuron-specific chromatin-remodeling complex. The structure of this gene is similar to that of the SS18 gene. Mutations in this gene are involved in amyotrophic lateral sclerosis (ALS). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]