WDSUB1 (NM_152528) Human Tagged ORF Clone

CAT#: RC212468

WDSUB1 (Myc-DDK-tagged)-Human WD repeat, sterile alpha motif and U-box domain containing 1 (WDSUB1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY426927) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225809 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified WDSUB1 protein (Cat# TP312468). The protein was produced from HEK293T cells transfected with WDSUB1 cDNA clone (Cat# RC212468) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDSUB1
• Synonyms: UBOX6; WDSAM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212468 representing NM_152528
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAACTGATTCACACATTAGCTGATCATGGTGACGATGTCAACTGCTGTGCCTTCTCCTTTTCCC
  TCTTGGCTACTTGCTCCTTGGACAAAACAATTCGCCTGTACTCGTTACGTGACTTTACTGAACTGCCACA
  TTCTCCATTGAAGTTTCATACCTATGCTGTCCACTGCTGCTGTTTCTCCCCTTCAGGACATATTTTGGCA
  TCGTGTTCAACAGATGGTACCACTGTCCTATGGAATACTGAAAATGGACAGATGCTGGCAGTGATGGAAC
  AGCCTAGTGGCAGCCCTGTGAGGGTTTGCCAGTTTTCCCCAGACTCCACGTGTTTGGCATCAGGGGCAGC
  TGATGGAACTGTGGTTTTGTGGAATGCACAGTCATACAAATTATATAGATGTGGTAGTGTTAAAGATGGC
  TCCTTGGCGGCATGTGCATTTTCTCCTAATGGAAGCTTCTTTGTCACTGGCTCCTCATGTGGTGATTTAA
  CAGTGTGGGATGATAAAATGAGGTGTCTGCATAGTGAAAAAGCACATGATCTTGGAATTACCTGCTGCGA
  TTTTTCTTCACAGCCAGTTTCTGATGGAGAACAAGGTCTTCAGTTTTTTCGACTGGCATCATGTGGTCAG
  GATTGCCAAGTCAAAATTTGGATTGTTTCTTTTACCCATATCTTAGGTTTTGAATTAAAATATAAAAGTA
  CACTGAGTGGGCACTGTGCTCCTGTTCTGGCTTGTGCTTTTTCCCATGATGGGCAGATGCTAGTCTCAGG
  GTCAGTGGATAAGTCTGTCATAGTATATGATACTAATACTGAGAATATACTTCACACATTGACTCAGCAC
  ACCAGGTATGTCACAACTTGTGCTTTTGCACCTAATACCCTTTTACTTGCTACTGGTTCAATGGACAAAA
  CAGTGAACATCTGGCAATTTGACCTGGAAACACTTTGCCAAGCAAGGCGCACAGAACATCAGCTGAAGCA
  ATTTACCGAAGATTGGTCAGAGGAGGATGTCTCAACATGGCTTTGTGCACAAGATTTAAAAGATCTTGTT
  GGTATTTTCAAGATGAATAACATTGATGGAAAAGAACTGTTGAATCTTACAAAAGAAAGTCTGGCTGATG
  ATTTGAAAATTGAATCTCTAGGACTGCGTAGTAAAGTGCTGAGGAAAATTGAAGAGCTCAGGACCAAGGT
  TAAATCCCTTTCTTCAGGAATTCCTGATGAATTTATATGTCCAATAACTAGAGAACTTATGAAAGATCCG
  GTCATCGCATCAGATGGCTATTCATATGAAAAGGAAGCAATGGAAAATTGGATCAGCAAAAAGAAACGTA
  CAAGTCCCATGACAAATCTTGTTCTTCCTTCAGCGGTACTTACACCAAATAGGACTCTGAAAATGGCCAT
  CAATAGATGGCTGGAGACACACCAAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212468 representing NM_152528
  Red=Cloning site Green=Tags(s)
  
  MVKLIHTLADHGDDVNCCAFSFSLLATCSLDKTIRLYSLRDFTELPHSPLKFHTYAVHCCCFSPSGHILA
  SCSTDGTTVLWNTENGQMLAVMEQPSGSPVRVCQFSPDSTCLASGAADGTVVLWNAQSYKLYRCGSVKDG
  SLAACAFSPNGSFFVTGSSCGDLTVWDDKMRCLHSEKAHDLGITCCDFSSQPVSDGEQGLQFFRLASCGQ
  DCQVKIWIVSFTHILGFELKYKSTLSGHCAPVLACAFSHDGQMLVSGSVDKSVIVYDTNTENILHTLTQH
  TRYVTTCAFAPNTLLLATGSMDKTVNIWQFDLETLCQARRTEHQLKQFTEDWSEEDVSTWLCAQDLKDLV
  GIFKMNNIDGKELLNLTKESLADDLKIESLGLRSKVLRKIEELRTKVKSLSSGIPDEFICPITRELMKDP
  VIASDGYSYEKEAMENWISKKKRTSPMTNLVLPSAVLTPNRTLKMAINRWLETHQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152528
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152528.1, NM_152528.2, NP_689741.1
• RefSeq Size: 1811 bp
• RefSeq ORF: 1431 bp
• Locus ID: 151525
• Domains: SAM, WD40, U-box
• Protein Families: Druggable Genome
• MW: 52.7 kDa