RBM5 (NM_005778) Human Tagged ORF Clone

CAT#: RC212543

RBM5 (Myc-DDK-tagged)-Human RNA binding motif protein 5 (RBM5), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401757) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212543 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RBM5 protein (Cat# TP312543). The protein was produced from HEK293T cells transfected with RBM5 cDNA clone (Cat# RC212543) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RBM5
• Synonyms: G15; H37; LUCA-15; LUCA15; RMB5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212543 representing NM_005778
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTCAGACAAAAGAGTGAGTAGAACAGAGCGTAGTGGAAGATACGGTTCCATCATAGACAGGGATG
  ACCGTGATGAGCGTGAATCCCGAAGCAGGCGGAGGGACTCAGATTACAAAAGATCTAGTGATGATCGGAG
  GGGTGATAGATATGATGACTACCGAGACTATGACAGTCCAGAGAGAGAGCGTGAAAGAAGGAACAGTGAC
  CGATCCGAAGATGGCTACCATTCAGATGGTGACTATGGTGAGCACGACTATAGGCATGACATCAGTGACG
  AGAGGGAGAGCAAGACCATCATGCTGCGCGGCCTTCCCATCACCATCACAGAGAGCGATATTCGAGAAAT
  GATGGAGTCCTTCGAAGGCCCTCAGCCTGCGGATGTGAGGCTGATGAAGAGGAAAACAGGTGTAAGCCGT
  GGTTTCGCCTTCGTGGAGTTTTATCACTTGCAAGATGCTACCAGCTGGATGGAAGCCAATCAGAAAAAGT
  TGGTGATTCAAGGAAAGCACATTGCAATGCATTATAGCAATCCCAGACCTAAGTTTGAAGATTGGCTTTG
  TAACAAGTGCTGCCTTAACAATTTCAGGAAAAGACTAAAATGCTTCCGATGTGGAGCAGACAAGTTTGAC
  TCTGAACAGGAAGTGCCTCCTGGAACCACAGAGTCGGTTCAGTCTGTGGATTACTACTGTGATACGATCA
  TTCTTCGGAACATAGCTCCGCACACTGTGGTGGATTCCATCATGACAGCACTGTCTCCTTACGCGTCTTT
  AGCTGTCAATAACATCCGCCTCATAAAAGACAAACAGACCCAGCAGAACAGAGGCTTCGCATTTGTGCAG
  CTGTCCTCTGCAATGGATGCTTCTCAGCTGCTTCAGATATTACAGAGTCTCCATCCTCCTTTGAAAATTG
  ATGGCAAAACTATTGGGGTTGATTTTGCAAAAAGTGCCAGAAAAGACTTGGTCCTCTCAGATGGTAACCG
  CGTCAGCGCCTTCTCTGTAGCTAGTACGGCTATTGCTGCTGCTCAGTGGTCATCCACCCAGTCTCAAAGT
  GGTGAAGGAGGCAGTGTTGACTACAGTTATCTGCAACCAGGTCAAGATGGCTATGCCCAATATGCTCAGT
  ATTCACAGGATTATCAGCAGTTTTATCAACAACAAGCTGGAGGATTGGAATCTGATGCATCATCTGCATC
  AGGCACAGCAGTGACCACCACCTCAGCGGCTGTAGTGTCCCAGAGTCCTCAGCTGTATAATCAAACCTCC
  AATCCACCTGGCTCTCCGACTGAGGAAGCACAGCCTAGCACTAGCACAAGTACACAGGCCCCAGCCGCTT
  CCCCTACTGGTGTAGTTCCTGGTACCAAATATGCAGTACCTGACACGTCCACTTACCAGTATGATGAATC
  TTCAGGATATTACTATGATCCGACAACAGGGCTCTATTATGACCCCAACTCGCAATACTACTATAATTCC
  TTGACCCAGCAGTACCTTTACTGGGATGGGGAAAAAGAGACCTACGTGCCAGCTGCAGAGTCTAGCTCCC
  ACCAGCAGTCGGGCCTGCCTCCTGCAAAAGAGGGGAAAGAGAAGAAGGAGAAACCCAAGAGCAAAACAGC
  CCAGCAGATTGCCAAAGACATGGAACGCTGGGCTAAGAGTTTGAATAAGCAGAAAGAAAACTTTAAAAAT
  AGCTTTCAGCCTGTCAATTCCTTGAGGGAAGAAGAAAGGAGAGAATCTGCTGCAGCAGACGCTGGCTTTG
  CTCTCTTTGAGAAGAAGGGAGCCTTAGCTGAAAGGCAGCAGCTCATCCCAGAATTGGTGCGAAATGGAGA
  TGAGGAGAATCCCCTCAAAAGGGGTCTGGTTGCTGCTTACAGTGGTGACAGTGACAATGAGGAGGAGCTG
  GTGGAGAGACTTGAGAGTGAGGAAGAGAAGCTAGCTGACTGGAAGAAGATGGCCTGTCTGCTCTGCCGGC
  GCCAGTTCCCGAACAAAGATGCCCTAGTCAGGCACCAGCAACTCTCAGACCTTCACAAGCAAAACATGGA
  CATCTACCGACGATCCAGGCTGAGCGAGCAGGAGCTGGAAGCCTTGGAGCTAAGGGAGAGAGAGATGAAA
  TACCGAGACCGAGCTGCAGAAAGACGGGAGAAGTACGGCATTCCAGAACCTCCAGAGCCCAAGCGCAAGA
  AGCAGTTTGATGCCGGCACTGTGAATTACGAGCAACCCACCAAAGATGGCATTGACCACAGTAACATTGG
  CAACAAGATGCTGCAGGCCATGGGCTGGCGGGAAGGCTCTGGCTTGGGACGAAAGTGTCAAGGCATTACG
  GCTCCCATTGAGGCTCAAGTTCGGCTAAAGGGAGCTGGCCTAGGAGCCAAAGGCAGCGCATATGGTTTGT
  CGGGCGCCGATTCCTACAAAGATGCTGTCCGGAAAGCCATGTTTGCCCGGTTCACTGAGATGGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212543 representing NM_005778
  Red=Cloning site Green=Tags(s)
  
  MGSDKRVSRTERSGRYGSIIDRDDRDERESRSRRRDSDYKRSSDDRRGDRYDDYRDYDSPERERERRNSD
  RSEDGYHSDGDYGEHDYRHDISDERESKTIMLRGLPITITESDIREMMESFEGPQPADVRLMKRKTGVSR
  GFAFVEFYHLQDATSWMEANQKKLVIQGKHIAMHYSNPRPKFEDWLCNKCCLNNFRKRLKCFRCGADKFD
  SEQEVPPGTTESVQSVDYYCDTIILRNIAPHTVVDSIMTALSPYASLAVNNIRLIKDKQTQQNRGFAFVQ
  LSSAMDASQLLQILQSLHPPLKIDGKTIGVDFAKSARKDLVLSDGNRVSAFSVASTAIAAAQWSSTQSQS
  GEGGSVDYSYLQPGQDGYAQYAQYSQDYQQFYQQQAGGLESDASSASGTAVTTTSAAVVSQSPQLYNQTS
  NPPGSPTEEAQPSTSTSTQAPAASPTGVVPGTKYAVPDTSTYQYDESSGYYYDPTTGLYYDPNSQYYYNS
  LTQQYLYWDGEKETYVPAAESSSHQQSGLPPAKEGKEKKEKPKSKTAQQIAKDMERWAKSLNKQKENFKN
  SFQPVNSLREEERRESAAADAGFALFEKKGALAERQQLIPELVRNGDEENPLKRGLVAAYSGDSDNEEEL
  VERLESEEEKLADWKKMACLLCRRQFPNKDALVRHQQLSDLHKQNMDIYRRSRLSEQELEALELREREMK
  YRDRAAERREKYGIPEPPEPKRKKQFDAGTVNYEQPTKDGIDHSNIGNKMLQAMGWREGSGLGRKCQGIT
  APIEAQVRLKGAGLGAKGSAYGLSGADSYKDAVRKAMFARFTEME
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005778
• ORF Size: 2445 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005778.1, NM_005778.2, NM_005778.3, NP_005769.1
• RefSeq Size: 3135 bp
• RefSeq ORF: 2448 bp
• Locus ID: 10181
• Domains: G-patch, RRM, zf-RanBP, zf-C2H2
• Protein Families: Druggable Genome
• MW: 92 kDa
• Gene Summary: This gene is a candidate tumor suppressor gene which encodes a nuclear RNA binding protein that is a component of the spliceosome A complex. The encoded protein plays a role in the induction of cell cycle arrest and apoptosis through pre-mRNA splicing of multiple target genes including the tumor suppressor protein p53. This gene is located within the tumor suppressor region 3p21.3, and may play a role in the inhibition of tumor transformation and progression of several malignancies including lung cancer. [provided by RefSeq, Oct 2011]