FIGLA (NM_001004311) Human Tagged ORF Clone

CAT#: RC212562

FIGLA (Myc-DDK-tagged)-Human folliculogenesis specific basic helix-loop-helix (FIGLA)

Product Images

Western blot validation of overexpression lysate (Cat# LY424087) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212562 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FIGLA protein (Cat# TP312562). The protein was produced from HEK293T cells transfected with FIGLA cDNA clone (Cat# RC212562) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FIGLA
• Synonyms: BHLHC8; FIGALPHA; POF6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212562 representing NM_001004311
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCGCGCCCGGCGTCCTAGATCCCCGCGCCGCGCCGCCCGCGCTCCTGGGCACCCCGCAAGCCG
  AGGTGCTGGAGGACGTGTTGCGGGAGCAGTTCGGGCCGCTGCCCCAGCTGGCCGCTGTCTGCCGGCTCAA
  GCGGCTGCCCTCGGGCGGCTACTCGTCCACTGAAAACCTCCAGTTGGTGCTGGAGCGGCGGCGTGTGGCC
  AACGCCAAGGAGCGTGAGCGGATAAAAAATCTCAACCGTGGTTTTGCCAGATTGAAGGCACTTGTGCCAT
  TTCTTCCCCAAAGCAGGAAGCCCAGCAAAGTTGATATCCTTAAAGGTGCGACTGAATATATACAGGTTCT
  CAGTGATCTTTTGGAAGGAGCCAAAGACTCAAAGAAACAAGACCCAGATGAGCAGAGCTATAGTAACAAC
  AGTTCTGAATCACATACATCCTCGGCAAGACAGCTGTCAAGAAACATCACCCAACATATCAGCTGTGCTT
  TCGGCTTGAAGAATGAAGAGGAAGGGCCTTGGGCAGATGGTGGCAGTGGTGAGCCAGCACACGCTTGTCG
  CCACAGTGTGATGTCTACGACTGAAATTATCTCCCCAACCAGAAGTCTGGATAGATTCCCAGAAGTAGAA
  CTGCTGAGTCACAGACTTCCACAAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212562 representing NM_001004311
  Red=Cloning site Green=Tags(s)
  
  MDPAPGVLDPRAAPPALLGTPQAEVLEDVLREQFGPLPQLAAVCRLKRLPSGGYSSTENLQLVLERRRVA
  NAKERERIKNLNRGFARLKALVPFLPQSRKPSKVDILKGATEYIQVLSDLLEGAKDSKKQDPDEQSYSNN
  SSESHTSSARQLSRNITQHISCAFGLKNEEEGPWADGGSGEPAHACRHSVMSTTEIISPTRSLDRFPEVE
  LLSHRLPQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004311
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004311.1, NM_001004311.2, NM_001004311.3, NP_001004311.1
• RefSeq Size: 724
• RefSeq ORF: 660
• Locus ID: 344018
• MW: 23.9 kDa
• Gene Summary: This gene encodes a protein that functions in postnatal oocyte-specific gene expression. The protein is a basic helix-loop-helix transcription factor that regulates multiple oocyte-specific genes, including genes involved in folliculogenesis and those that encode the zona pellucida. Mutations in this gene cause premature ovarian failure type 6. [provided by RefSeq, Sep 2009]