MSK2 / RSK-B (RPS6KA4) (NM_003942) Human Tagged ORF Clone

CAT#: RC212570

RPS6KA4 (Myc-DDK-tagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 4 (RPS6KA4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418335) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212570 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RPS6KA4 protein (Cat# TP312570). The protein was produced from HEK293T cells transfected with RPS6KA4 cDNA clone (Cat# RC212570) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPS6KA4
• Synonyms: MSK2; RSK-B; S6K-alpha-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212570 representing NM_003942
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACGAGGACGACGATGAGAGCTGCGCCGTGGAGCTGCGGATCACAGAAGCCAACCTGACCGGGC
  ACGAGGAGAAGGTGAGCGTGGAGAACTTCGAGCTGCTCAAGGTGCTGGGCACGGGAGCCTACGGCAAGGT
  GTTCCTGGTGCGGAAGGCGGGCGGGCACGACGCGGGGAAGCTGTACGCCATGAAGGTGCTGCGCAAGGCG
  GCGCTGGTGCAGCGCGCCAAGACGCAAGAGCACACGCGCACCGAGCGCTCGGTGCTGGAGCTGGTGCGCC
  AGGCGCCCTTCCTGGTCACGCTGCACTACGCTTTCCAGACGGATGCCAAGCTGCACCTCATCCTGGACTA
  TGTGAGCGGCGGGGAGATGTTCACCCACCTCTACCAGCGCCAGTACTTCAAGGAGGCTGAGGTGCGCGTG
  TATGGGGGTGAGATCGTGCTGGCCCTGGAACACCTGCACAAGCTCGGCATCATTTACCGAGACCTGAAAC
  TGGAGAATGTGCTGCTGGACTCCGAGGGCCACATTGTCCTCACGGACTTCGGGCTGAGCAAGGAGTTCCT
  GACGGAGGAGAAAGAGCGGACCTTCTCCTTCTGTGGCACCATCGAGTACATGGCCCCCGAAATCATCCGT
  AGCAAGACGGGGCATGGCAAGGCTGTGGACTGGTGGAGCCTGGGCATCTTGCTCTTCGAGCTGCTGACGG
  GGGCCTCGCCCTTCACCCTGGAGGGCGAGAGGAACACGCAGGCTGAGGTGTCTCGACGGATCCTGAAGTG
  CTCCCCTCCCTTCCCCCCTCGGATCGGGCCCGTGGCGCAGGACCTGCTGCAGCGGCTGCTTTGTAAGGAT
  CCTAAGAAGCGATTGGGCGCGGGGCCCCAGGGGGCACAAGAAGTCCGGAACCATCCCTTCTTCCAGGGCC
  TCGATTGGGTGGCTCTGGCTGCCAGGAAGATTCCAGCCCCATTCCGGCCCCAAATCCGCTCAGAGCTGGA
  TGTGGGCAACTTTGCGGAGGAATTCACTCGGCTGGAGCCTGTCTACTCACCCCCTGGCAGCCCCCCACCT
  GGGGACCCCCGAATCTTTCAGGGATACTCCTTTGTGGCACCCTCCATTCTCTTTGACCACAACAACGCGG
  TGATGACCGATGGGCTGGAAGCGCCTGGTGCTGGAGACCGGCCAGGTCGGGCAGCGGTGGCCAGGAGCGC
  TATGATGCAGGACTCGCCCTTCTTCCAGCAGTACGAGCTGGACCTGCGGGAGCCTGCGCTGGGCCAGGGC
  AGCTTTTCTGTGTGTCGCCGCTGCCGCCAGCGCCAGAGCGGCCAGGAGTTCGCAGTCAAGATCCTCAGTC
  GCAGGCTGGAGGCGAACACGCAGCGCGAAGTGGCTGCCCTGCGCCTGTGCCAGTCACACCCCAACGTGGT
  GAATCTGCACGAGGTGCATCACGACCAGCTGCACACGTACCTGGTCCTGGAGCTGCTGCGGGGCGGGGAG
  CTGCTGGAGCACATCCGCAAGAAGCGGCACTTCAGCGAGTCGGAAGCAAGCCAGATCCTGCGCAGCCTCG
  TGTCGGCCGTGAGCTTCATGCACGAGGAGGCGGGCGTGGTGCACCGCGACCTCAAGCCGGAGAACATCCT
  GTACGCCGACGACACGCCCGGGGCCCCGGTGAAAATCATCGACTTCGGGTTCGCGCGGTTGCGGCCGCAG
  AGTCCCGGGGTGCCCATGCAGACGCCCTGCTTCACGCTGCAGTACGCTGCCCCCGAGCTGCTGGCGCAGC
  AGGGCTACGACGAGTCCTGCGACCTCTGGAGCCTGGGCGTCATTCTGTACATGATGCTGTCGGGGCAGGT
  CCCCTTCCAGGGGGCCTCTGGCCAGGGCGGGCAGAGCCAGGCGGCCGAGATCATGTGCAAAATCCGCGAG
  GGGCGCTTCTCCCTTGACGGGGAGGCCTGGCAGGGTGTATCCGAGGAAGCCAAGGAGCTGGTCCGAGGGC
  TCCTGACCGTGGACCCCGCCAAGCGGCTGAAGCTCGAGGGACTGCGGGGCAGCTCGTGGCTGCAGGACGG
  CAGCGCGCGCTCCTCGCCCCCGCTCCGGACGCCCGACGTGCTCGAGTCCTCTGGGCCCGCAGTGCGCTCG
  GGTCTCAACGCCACCTTCATGGCATTCAACCGGGGCAAGCGGGAGGGCTTCTTCCTGAAGAGCGTGGAGA
  ATGCACCCCTGGCCAAGCGGCGGAAGCAGAAGCTGCGGAGCGCCACCGCCTCCCGCCGGGGCTCCCCTGC
  ACCAGCCAACCCGGGCCGAGCCCCCGTCGCCTCCAAAGGGGCCCCCCGCCGAGCCAACGGCCCCCTGCCC
  CCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212570 representing NM_003942
  Red=Cloning site Green=Tags(s)
  
  MGDEDDDESCAVELRITEANLTGHEEKVSVENFELLKVLGTGAYGKVFLVRKAGGHDAGKLYAMKVLRKA
  ALVQRAKTQEHTRTERSVLELVRQAPFLVTLHYAFQTDAKLHLILDYVSGGEMFTHLYQRQYFKEAEVRV
  YGGEIVLALEHLHKLGIIYRDLKLENVLLDSEGHIVLTDFGLSKEFLTEEKERTFSFCGTIEYMAPEIIR
  SKTGHGKAVDWWSLGILLFELLTGASPFTLEGERNTQAEVSRRILKCSPPFPPRIGPVAQDLLQRLLCKD
  PKKRLGAGPQGAQEVRNHPFFQGLDWVALAARKIPAPFRPQIRSELDVGNFAEEFTRLEPVYSPPGSPPP
  GDPRIFQGYSFVAPSILFDHNNAVMTDGLEAPGAGDRPGRAAVARSAMMQDSPFFQQYELDLREPALGQG
  SFSVCRRCRQRQSGQEFAVKILSRRLEANTQREVAALRLCQSHPNVVNLHEVHHDQLHTYLVLELLRGGE
  LLEHIRKKRHFSESEASQILRSLVSAVSFMHEEAGVVHRDLKPENILYADDTPGAPVKIIDFGFARLRPQ
  SPGVPMQTPCFTLQYAAPELLAQQGYDESCDLWSLGVILYMMLSGQVPFQGASGQGGQSQAAEIMCKIRE
  GRFSLDGEAWQGVSEEAKELVRGLLTVDPAKRLKLEGLRGSSWLQDGSARSSPPLRTPDVLESSGPAVRS
  GLNATFMAFNRGKREGFFLKSVENAPLAKRRKQKLRSATASRRGSPAPANPGRAPVASKGAPRRANGPLP
  PS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003942
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003942.1, NM_003942.2, NP_003933.1
• RefSeq Size: 3149 bp
• RefSeq ORF: 2319 bp
• Locus ID: 8986
• Domains: pkinase, S_TK_X
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: MAPK signaling pathway, Neurotrophin signaling pathway
• MW: 85.4 kDa
• Gene Summary: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]