Espin (ESPN) (NM_031475) Human Tagged ORF Clone

CAT#: RC212577

ESPN (Myc-DDK-tagged)-Human espin (ESPN)

Product Images

Western blot validation of overexpression lysate (Cat# LY410481) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212577 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ESPN protein (Cat# TP312577). The protein was produced from HEK293T cells transfected with ESPN cDNA clone (Cat# RC212577) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ESPN
• Synonyms: DFNB36; LP2654
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212577 representing NM_031475
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCTGGAGCAGGCGCTGCAGGCGGCGCGGCAGGGCGAGCTGGACGTGCTGAGGTCGCTGCACGCCG
  CAGGCCTCCTGGGGCCCTCGCTGCGCGACCCGCTGGACGCGCTGCCCGTGCACCACGCGGCCCGCGCTGG
  GAAGCTGCACTGTCTGCGCTTCCTGGTGGAGGAAGCCGCCCTCCCCGCCGCGGCCCGCGCCCGCAACGGC
  GCCACACCGGCCCACGACGCCTCCGCCACCGGCCACCTCGCCTGCCTGCAGTGGCTGCTGTCGCAGGGCG
  GCTGCAGAGTGCAGGACAAAGACAATTCTGGTGCCACAGTCTTGCATCTGGCTGCCCGCTTCGGCCACCC
  CGAGGTGGTGAACTGGCTCTTGCATCATGGCGGTGGGGACCCCACCGCGGCCACAGACATGGGCGCCCTG
  CCTATCCACTACGCTGCCGCCAAAGGAGACTTCCCCTCCCTGAGGCTTCTCGTCGAGCACTACCCTGAGG
  GAGTGAATGCCCAAACCAAGAACGGTGCCACGCCCCTGTACCTGGCGTGCCAGGAGGGCCACCTGGAGGT
  GACCCAGTACCTGGTGCAGGAATGCGGCGCAGACCCGCACGCGCGCGCCCACGACGGCATGACCCCGCTG
  CACGCCGCGGCGCAGATGGGCCACAGCCCAGTCATCGTGTGGTTGGTGAGCTGCACCGACGTGAGCCTGT
  CCGAGCAGGACAAAGACGGCGCCACCGCCATGCACTTCGCGGCGAGCCGCGGCCACACCAAGGTGCTCAG
  CTGGCTGCTGCTGCACGGCGGGGAGATCTCGGCTGACCTGTGGGGCGGGACCCCGCTGCACGACGCCGCC
  GAGAACGGGGAGCTAGAGTGCTGCCAGATCCTGGTAGTGAACGGCGCGGAGCTGGACGTCCGCGACCGCG
  ACGGGTACACGGCCGCCGACCTGTCGGACTTCAACGGCCACAGCCACTGCACCCGCTACCTGCGCACGGT
  GGAGAACCTGAGCGTGGAGCACCGCGTGCTTTCCCGGGATCCATCCGCAGAGCTGGAGGCTAAGCAGCCG
  GATTCAGGCATGTCCTCACCCAATACCACGGTGTCGGTCCAGCCGCTGAACTTTGACCTCAGCTCGCCTA
  CCAGCACCCTCTCCAACTACGACTCCTGCTCCTCCAGCCACTCCAGCATCAAGGGCCAGCACCCTCCATG
  TGGGCTTTCCAGCGCTAGAGCTGCAGACATACAGAGCTACATGGACATGCTGAACCCGGAGCTGGGCCTG
  CCTCGGGGCACGATTGGGAAGCCCACACCCCCACCACCCCCACCCAGCTTCCCCCCGCCACCCCCGCCCC
  CAGGCACCCAACTGCCCCCACCCCCACCTGGCTACCCAGCTCCCAAGCCTCCTGTAGGACCACAGGCAGC
  TGACATCTACATGCAGACCAAGAACAAACTCCGCCACGTGGAGACAGAGGCCCTCAAGAAGGAGCTGAGC
  TCCTGTGACGGCCACGACGGGCTGCGGAGGCAGGACTCCAGCCGCAAGCCCCGCGCCTTCAGCAAGCAGC
  CCAGCACGGGGGACTACTACCGGCAGCTGGGCCGCTGCCCCGGCGAGACGCTGGCCGCACGCCCGGGCAT
  GGCGCACAGCGAGGAGGTGCGTGCCCGCCAGCCCGCGCGCGCCGGCTGCCCGCGCCTCGGCCCTGCCGCC
  CGCGGCTCACTCGAAGGCCCCTCCGCTCCCCCGCAGGCGGCGCTGCTTCCTGGGAACCATGTTCCTAACG
  GCTGCGCCGCGGACCCCAAGGCGTCCAGGGAGCTGCCACCGCCGCCCCCACCGCCGCCGCCGCCCCTGCC
  GGAGGCCGCGAGTTCGCCACCGCCGGCCCCGCCTCTGCCCCTCGAGAGCGCTGGCCCTGGCTGCGGGCAG
  CGCCGCTCCTCCTCGTCCACCGGCAGCACCAAGTCTTTCAACATGATGTCCCCGACGGGCGACAACTCGG
  AGCTACTGGCTGAGATTAAGGCAGGCAAGAGCCTGAAGCCGACGCCCCAGAGCAAGGGGCTGACCACAGT
  GTTCTCAGGCATCGGGCAGCCGGCCTTCCAGCCCGATTCGCCGCTGCCTTCTGTGTCACCTGCACTGTCA
  CCAGTCCGGAGCCCCACACCGCCAGCTGCGGGGTTTCAGCCGCTGCTCAATGGAAGCTTGGTTCCCGTGC
  CGCCCACTACTCCTGCGCCGGGAGTGCAGCTGGACGTGGAGGCTCTCATCCCCACGCACGATGAGCAGGG
  CCGGCCCATCCCCGAGTGGAAGCGCCAGGTGATGGTGCGCAAGATGCAGCTGAAGATGCAGGAGGAGGAG
  GAGCAGAGGCGGAAGGAGGAGGAGGAGGAGGCCCGGCTGGCCAGCATGCCCGCCTGGAGGCGGGACCTCC
  TGCGGAAGAAGCTGGAAGAAGAGAGGGAGCAGAAGCGGAAAGAGGAGGAGCGACAGAAGCAGGAGGAGCT
  GCGGCGGGAGAAGGAACAGTCAGAGAAGCTGCGGACGCTGGGCTACGATGAGAGCAAGCTGGCGCCCTGG
  CAGCGACAGGTCATCCTGAAGAAGGGGGACATCGCTAAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212577 representing NM_031475
  Red=Cloning site Green=Tags(s)
  
  MALEQALQAARQGELDVLRSLHAAGLLGPSLRDPLDALPVHHAARAGKLHCLRFLVEEAALPAAARARNG
  ATPAHDASATGHLACLQWLLSQGGCRVQDKDNSGATVLHLAARFGHPEVVNWLLHHGGGDPTAATDMGAL
  PIHYAAAKGDFPSLRLLVEHYPEGVNAQTKNGATPLYLACQEGHLEVTQYLVQECGADPHARAHDGMTPL
  HAAAQMGHSPVIVWLVSCTDVSLSEQDKDGATAMHFAASRGHTKVLSWLLLHGGEISADLWGGTPLHDAA
  ENGELECCQILVVNGAELDVRDRDGYTAADLSDFNGHSHCTRYLRTVENLSVEHRVLSRDPSAELEAKQP
  DSGMSSPNTTVSVQPLNFDLSSPTSTLSNYDSCSSSHSSIKGQHPPCGLSSARAADIQSYMDMLNPELGL
  PRGTIGKPTPPPPPPSFPPPPPPPGTQLPPPPPGYPAPKPPVGPQAADIYMQTKNKLRHVETEALKKELS
  SCDGHDGLRRQDSSRKPRAFSKQPSTGDYYRQLGRCPGETLAARPGMAHSEEVRARQPARAGCPRLGPAA
  RGSLEGPSAPPQAALLPGNHVPNGCAADPKASRELPPPPPPPPPPLPEAASSPPPAPPLPLESAGPGCGQ
  RRSSSSTGSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGIGQPAFQPDSPLPSVSPALS
  PVRSPTPPAAGFQPLLNGSLVPVPPTTPAPGVQLDVEALIPTHDEQGRPIPEWKRQVMVRKMQLKMQEEE
  EQRRKEEEEEARLASMPAWRRDLLRKKLEEEREQKRKEEERQKQEELRREKEQSEKLRTLGYDESKLAPW
  QRQVILKKGDIAKY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031475
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031475.1, NM_031475.2, NP_113663.1, NP_113663.2
• RefSeq Size: 3542
• RefSeq ORF: 2565
• Locus ID: 83715
• MW: 91.6 kDa
• Gene Summary: This gene encodes a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Mutations in this gene are associated with autosomal recessive neurosensory deafness, and autosomal dominant sensorineural deafness without vestibular involvement. [provided by RefSeq, Nov 2009]