PTCHD1 (NM_173495) Human Tagged ORF Clone

CAT#: RC212638

PTCHD1 (Myc-DDK-tagged)-Human patched domain containing 1 (PTCHD1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTCHD1
• Synonyms: AUTSX4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212638 representing NM_173495
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGCAGGTTCTGCACAGGGGCTTGAGGACGTGTTTCTCCCGGCTCGGCCACTTCATTGCCAGTC
  ACCCTGTCTTCTTCGCCTCGGCGCCGGTGCTCATCTCCATCCTGCTCGGCGCCAGCTTCAGCCGCTACCA
  GGTCGAGGAGAGCGTGGAGCACCTGCTGGCGCCCCAGCACAGCCTGGCCAAGATCGAGCGCAACCTCGTT
  AACAGCCTCTTCCCGGTCAACCGCTCCAAGCACCGTCTCTACTCGGACCTGCAGACCCCCGGGCGCTACG
  GCCGGGTCATCGTCACCTCCTTCCAGAAAGCCAACATGCTGGACCAGCATCACACCGACCTGATCTTAAA
  GTTGCATGCTGCTGTCACCAAGATCCAGGTTCCAAGGCCTGGTTTTAATTACACGTTTGCCCATATATGT
  ATCCTGAATAATGATAAGACTTGCATCGTGGATGACATAGTGCACGTCCTGGAAGAGCTAAAGAATGCTC
  GGGCCACCAATCGGACCAATTTTGCTATCACATACCCAATCACTCACTTAAAGGACGGGAGGGCTGTGTA
  CAATGGGCACCAGCTTGGGGGCGTCACTGTGCACAGCAAAGACCGGGTGAAATCTGCAGAGGCCATCCAG
  CTCACCTACTACCTGCAGTCAATCAACAGTCTCAATGACATGGTGGCTGAGAGGTGGGAGTCCAGCTTCT
  GCGACACTGTCAGACTGTTTCAGAAATCCAACAGCAAAGTCAAAATGTACCCTTACACGTCCTCCTCACT
  GAGGGAAGATTTCCAGAAGACCAGCCGCGTATCAGAACGTTACCTGGTCACCAGCCTGATTCTGGTGGTT
  ACCATGGCCATCCTGTGTTGCTCTATGCAGGACTGCGTCCGCAGCAAACCCTGGCTAGGCCTGCTCGGAT
  TGGTGACCATAAGCCTGGCCACTCTCACTGCAGCCGGGATCATCAATCTTACTGGTGGGAAATATAATTC
  CACCTTCCTGGGAGTCCCTTTCGTCATGCTAGGTCATGGATTATATGGGACTTTTGAAATGTTATCCTCC
  TGGAGGAAAACTAGAGAAGACCAACATGTTAAAGAGAGAACTGCAGCAGTCTATGCAGACTCCATGCTCT
  CCTTTTCTCTCACCACTGCCATGTACCTGGTCACCTTTGGCATAGGGGCCAGCCCTTTCACGAACATTGA
  GGCAGCCAGGATTTTCTGCTGCAATTCCTGTATTGCAATCTTCTTCAACTACCTCTATGTACTCTCGTTT
  TATGGTTCCAGCCTAGTGTTCACTGGCTACATAGAAAACAATTACCAGCATAGTATCTTCTGTAGAAAAG
  TCCCAAAGCCTGAGGCATTGCAGGAGAAGCCGGCATGGTACAGGTTTCTCCTGACGGCCAGATTCAGTGA
  GGACACAGCTGAAGGCGAGGAAGCGAACACTTACGAGAGTCACCTATTGGTATGTTTCCTCAAACGCTAT
  TACTGTGACTGGATAACCAACACCTATGTCAAGCCTTTTGTAGTTCTCTTTTACCTTATTTATATTTCCT
  TTGCCTTAATGGGCTATCTGCAGGTCAGTGAAGGGTCAGACCTTAGTAACATTGTAGCAACCGCGACACA
  AACCATTGAGTACACTACTGCCCAGCAAAAGTACTTCAGCAACTACAGTCCTGTGATTGGGTTTTACATA
  TATGAGTCTATAGAATACTGGAACACTAGTGTCCAAGAAGATGTTCTAGAATACACCAAGGGGTTTGTGC
  GGATATCCTGGTTTGAGAGCTATTTAAATTACCTTCGGAAACTCAATGTATCCACTGGCTTGCCTAAGAA
  AAATTTCACAGACATGTTGAGGAATTCCTTTCTGAAAGCCCCTCAATTTTCACATTTTCAAGAGGACATC
  ATCTTCTCTAAAAAATACAATGATGAGGTCGATGTAGTGGCCTCCAGAATGTTTTTGGTGGCCAAGACCA
  TGGAAACAAACAGAGAAGAACTCTATGATCTCTTGGAAACCCTGAGGAGACTTTCTGTCACCTCCAAGGT
  GAAGTTCATCGTCTTCAATCCGTCCTTTGTATACATGGATCGATATGCCTCCTCTCTGGGAGCCCCCCTG
  CACAACTCCTGCATCAGTGCTTTGTTCCTGCTCTTCTTCTCGGCATTCCTGGTGGCAGATTCACTGATTA
  ACGTCTGGATCACTCTCACAGTTGTGTCCGTGGAGTTTGGAGTGATAGGTTTCATGACATTATGGAAAGT
  AGAACTGGACTGCATTTCTGTGCTATGCTTAATTTATGGAATTAATTACACAATTGACAATTGTGCTCCA
  ATGTTATCCACATTTGTTCTGGGCAAGGATTTCACAAGAACTAAATGGGTAAAAAATGCCCTGGAAGTGC
  ATGGGGTAGCTATTTTACAGAGTTACCTCTGCTATATTGTTGGTCTGATTCCTCTTGCAGCTGTGCCTTC
  AAATCTGACCTGTACACTGTTCAGGTGCTTGTTTTTAATAGCATTTGTCACCTTCTTTCACTGCTTTGCC
  ATTTTACCTGTGATACTGACTTTCCTGCCACCCTCTAAGAAAAAAAGGAAAGAGAAGAAAAATCCTGAGA
  ACCGGGAGGAAATTGAGTGTGTAGAAATGGTAGATATCGATAGTACCCGTGTGGTTGACCAAATTACAAC
  AGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212638 representing NM_173495
  Red=Cloning site Green=Tags(s)
  
  MLRQVLHRGLRTCFSRLGHFIASHPVFFASAPVLISILLGASFSRYQVEESVEHLLAPQHSLAKIERNLV
  NSLFPVNRSKHRLYSDLQTPGRYGRVIVTSFQKANMLDQHHTDLILKLHAAVTKIQVPRPGFNYTFAHIC
  ILNNDKTCIVDDIVHVLEELKNARATNRTNFAITYPITHLKDGRAVYNGHQLGGVTVHSKDRVKSAEAIQ
  LTYYLQSINSLNDMVAERWESSFCDTVRLFQKSNSKVKMYPYTSSSLREDFQKTSRVSERYLVTSLILVV
  TMAILCCSMQDCVRSKPWLGLLGLVTISLATLTAAGIINLTGGKYNSTFLGVPFVMLGHGLYGTFEMLSS
  WRKTREDQHVKERTAAVYADSMLSFSLTTAMYLVTFGIGASPFTNIEAARIFCCNSCIAIFFNYLYVLSF
  YGSSLVFTGYIENNYQHSIFCRKVPKPEALQEKPAWYRFLLTARFSEDTAEGEEANTYESHLLVCFLKRY
  YCDWITNTYVKPFVVLFYLIYISFALMGYLQVSEGSDLSNIVATATQTIEYTTAQQKYFSNYSPVIGFYI
  YESIEYWNTSVQEDVLEYTKGFVRISWFESYLNYLRKLNVSTGLPKKNFTDMLRNSFLKAPQFSHFQEDI
  IFSKKYNDEVDVVASRMFLVAKTMETNREELYDLLETLRRLSVTSKVKFIVFNPSFVYMDRYASSLGAPL
  HNSCISALFLLFFSAFLVADSLINVWITLTVVSVEFGVIGFMTLWKVELDCISVLCLIYGINYTIDNCAP
  MLSTFVLGKDFTRTKWVKNALEVHGVAILQSYLCYIVGLIPLAAVPSNLTCTLFRCLFLIAFVTFFHCFA
  ILPVILTFLPPSKKKRKEKKNPENREEIECVEMVDIDSTRVVDQITTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173495
• ORF Size: 2664 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_173495.2, NP_775766.2
• RefSeq Size: 5305
• RefSeq ORF: 2667
• Locus ID: 139411
• Protein Families: Druggable Genome, Transmembrane
• MW: 101.2 kDa
• Gene Summary: This gene encodes a membrane protein with a patched domain. The encoded protein is similar to Drosophila proteins which act as receptors for the morphogen sonic hedgehog. Deletions in this gene, which is located on the X chromosome, are associated with intellectual disability and autism (PMID: 21091464, PMID: 20844286). [provided by RefSeq, Aug 2011]