LLGL2 (NM_001015002) Human Tagged ORF Clone

CAT#: RC212661

LLGL2 (Myc-DDK-tagged)-Human lethal giant larvae homolog 2 (Drosophila) (LLGL2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY423111) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212661 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LLGL2
• Synonyms: HGL; Hugl-2; LGL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212661 representing NM_001015002
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGGTTCCTGAGGCCAGGGCATGACCCTGTGCGGGAGAGGCTCAAGCGGGACCTGTTCCAGTTTA
  ACAAGACGGTGGAGCATGGCTTCCCGCACCAGCCCAGCGCCCTCGGCTACAGCCCGTCCCTGCGCATCCT
  GGCCATCGGCACCCGTTCTGGAGCCATCAAGCTCTACGGAGCCCCAGGCGTGGAGTTCATGGGGCTGCAC
  CAGGAGAACAACGCTGTGACGCAGATCCACCTCCTGCCCGGCCAGTGCCAGCTGGTCACCCTGCTGGATG
  ACAACAGCCTGCACCTTTGGAGCCTGAAGGTCAAGGGCGGGGCATCGGAGCTGCAGGAGGATGAGAGCTT
  CACACTGCGTGGACCCCCAGGGGCTGCCCCCAGTGCCACACAGATCACCGTGGTCCTGCCACATTCCTCC
  TGCGAGCTGCTCTACCTGGGCACCGAGAGTGGCAACGTGTTTGTGGTGCAGCTGCCAGCTTTTCGTGCGC
  TGGAGGACCGGACCATCAGCTCGGACGCGGTGCTGCAGCGGTTGCCAGAGGAGGCCCGCCACCGGCGTGT
  GTTCGAGATGGTGGAGGCACTGCAGGAGCACCCTCGAGACCCCAACCAGATCCTGATCGGCTACAGCCGA
  GGCCTCGTTGTCATCTGGGACCTACAGGGCAGCCGCGTGCTCTACCACTTCCTCAGCAGCCAGCAACTGG
  AGAACATCTGGTGGCAGCGGGACGGCCGCCTGCTCGTCAGCTGTCACTCTGACGGCAGCTACTGCCAGTG
  GCCCGTGTCCAGCGAAGCCCAGCAACCAGAGCCCCTCCGCAGCCTCGTGCCTTACGGTCCCTTTCCTTGC
  AAAGCGATTACCAGAATCCTCTGGCTGACCACTAGGCAGGGGTTGCCCTTCACCATCTTCCAGGGTGGCA
  TGCCACGGGCCAGCTACGGGGACCGCCACTGCATCTCAGTGATCCACGATGGCCAGCAGACGGCCTTCGA
  CTTCACCTCCCGTGTCATCGGCTTCACTGTCCTCACAGAGGCAGACCCTGCAGCCAGTAGGAGAGCTTCG
  GGAGTGGGTGCCCAGGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212661 representing NM_001015002
  Red=Cloning site Green=Tags(s)
  
  MRRFLRPGHDPVRERLKRDLFQFNKTVEHGFPHQPSALGYSPSLRILAIGTRSGAIKLYGAPGVEFMGLH
  QENNAVTQIHLLPGQCQLVTLLDDNSLHLWSLKVKGGASELQEDESFTLRGPPGAAPSATQITVVLPHSS
  CELLYLGTESGNVFVVQLPAFRALEDRTISSDAVLQRLPEEARHRRVFEMVEALQEHPRDPNQILIGYSR
  GLVVIWDLQGSRVLYHFLSSQQLENIWWQRDGRLLVSCHSDGSYCQWPVSSEAQQPEPLRSLVPYGPFPC
  KAITRILWLTTRQGLPFTIFQGGMPRASYGDRHCISVIHDGQQTAFDFTSRVIGFTVLTEADPAASRRAS
  GVGAQG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001015002
• ORF Size: 1068 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001015002.1, NP_001015002.1
• RefSeq Size: 1440 bp
• RefSeq ORF: 1071 bp
• Locus ID: 3993
• Cytogenetics: 17q25.1
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• MW: 39.5 kDa
• Gene Summary: 'The lethal (2) giant larvae protein of Drosophila plays a role in asymmetric cell division, epithelial cell polarity, and cell migration. This human gene encodes a protein similar to lethal (2) giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'