PIM3 (NM_001001852) Human Tagged ORF Clone
CAT#: RC212690
PIM3 (Myc-DDK-tagged)-Human pim-3 oncogene (PIM3)
"NM_001001852" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PIM3 |
Synonyms | pim-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC212690 representing NM_001001852
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTCTCCAAGTTCGGCTCCCTGGCGCACCTCTGCGGGCCCGGCGGCGTGGACCACCTCCCGGTGA AGATCCTGCAGCCAGCCAAGGCGGACAAGGAGAGCTTCGAGAAGGCGTACCAGGTGGGCGCCGTGCTGGG TAGCGGCGGCTTCGGCACGGTCTACGCGGGTAGCCGCATCGCCGACGGGCTCCCGGTGGCTGTGAAGCAC GTGGTGAAGGAGCGGGTGACCGAGTGGGGCAGCCTGGGCGGCGCGACCGTGCCCCTGGAGGTGGTGCTGC TGCGCAAGGTGGGCGCGGCGGGCGGCGCGCGCGGCGTCATCCGCCTGCTGGACTGGTTCGAGCGGCCCGA CGGCTTCCTGCTGGTGCTGGAGCGGCCCGAGCCGGCGCAGGACCTCTTCGACTTTATCACGGAGCGCGGC GCCCTGGACGAGCCGCTGGCGCGCCGCTTCTTCGCGCAGGTGCTGGCCGCCGTGCGCCACTGCCACAGCT GCGGGGTCGTGCACCGCGACATTAAGGACGAAAATCTGCTTGTGGACCTGCGCTCCGGAGAGCTCAAGCT CATCGACTTCGGTTCGGGTGCGCTGCTCAAGGACACGGTCTACACCGACTTCGACGGCACCCGAGTGTAC AGCCCCCCGGAGTGGATCCGCTACCACCGCTACCACGGGCGCTCGGCCACCGTGTGGTCGCTGGGCGTGC TTCTCTACGATATGGTGTGTGGGGACATCCCCTTCGAGCAGGACGAGGAGATCCTCCGAGGCCGCCTGCT CTTCCGGAGGAGGGTCTCTCCAGAGTGCCAGCAGCTGATCCGGTGGTGCCTGTCCCTGCGGCCCTCAGAG CGGCCGTCGCTGGATCAGATTGCGGCCCATCCCTGGATGCTGGGGGCTGACGGGGGCGCCCCGGAGAGCT GTGACCTGCGGCTGTGCACCCTCGACCCTGATGACGTGGCCAGCACCACGTCCAGCAGCGAGAGCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212690 representing NM_001001852
Red=Cloning site Green=Tags(s) MLLSKFGSLAHLCGPGGVDHLPVKILQPAKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVKH VVKERVTEWGSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERG ALDEPLARRFFAQVLAAVRHCHSCGVVHRDIKDENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVY SPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSE RPSLDQIAAHPWMLGADGGAPESCDLRLCTLDPDDVASTTSSSESL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001001852 |
ORF Size | 978 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001001852.1, NM_001001852.2, NM_001001852.3, NP_001001852.1 |
RefSeq Size | 2392 bp |
RefSeq ORF | 981 bp |
Locus ID | 415116 |
Protein Families | Druggable Genome, Protein Kinase |
MW | 35.7 kDa |
Gene Summary | The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. [provided by RefSeq, Jun 2012] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC300319 | PIM3 (untagged)-Human pim-3 oncogene (PIM3) |
USD 660.00 |
|
RG212690 | PIM3 (GFP-tagged) - Human pim-3 oncogene (PIM3) |
USD 460.00 |
|
RC212690L1 | Lenti ORF clone of Human pim-3 oncogene (PIM3), Myc-DDK-tagged |
USD 768.00 |
|
RC212690L2 | Lenti ORF clone of Human pim-3 oncogene (PIM3), mGFP tagged |
USD 620.00 |
|
RC212690L3 | Lenti ORF clone of Human pim-3 oncogene (PIM3), Myc-DDK-tagged |
USD 620.00 |
|
RC212690L4 | Lenti ORF clone of Human pim-3 oncogene (PIM3), mGFP tagged |
USD 620.00 |
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