PIM3 (NM_001001852) Human Tagged ORF Clone

CAT#: RG212690

PIM3 (GFP-tagged) - Human pim-3 oncogene (PIM3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIM3
• Synonyms: pim-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212690 representing NM_001001852
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCTCCAAGTTCGGCTCCCTGGCGCACCTCTGCGGGCCCGGCGGCGTGGACCACCTCCCGGTGA
  AGATCCTGCAGCCAGCCAAGGCGGACAAGGAGAGCTTCGAGAAGGCGTACCAGGTGGGCGCCGTGCTGGG
  TAGCGGCGGCTTCGGCACGGTCTACGCGGGTAGCCGCATCGCCGACGGGCTCCCGGTGGCTGTGAAGCAC
  GTGGTGAAGGAGCGGGTGACCGAGTGGGGCAGCCTGGGCGGCGCGACCGTGCCCCTGGAGGTGGTGCTGC
  TGCGCAAGGTGGGCGCGGCGGGCGGCGCGCGCGGCGTCATCCGCCTGCTGGACTGGTTCGAGCGGCCCGA
  CGGCTTCCTGCTGGTGCTGGAGCGGCCCGAGCCGGCGCAGGACCTCTTCGACTTTATCACGGAGCGCGGC
  GCCCTGGACGAGCCGCTGGCGCGCCGCTTCTTCGCGCAGGTGCTGGCCGCCGTGCGCCACTGCCACAGCT
  GCGGGGTCGTGCACCGCGACATTAAGGACGAAAATCTGCTTGTGGACCTGCGCTCCGGAGAGCTCAAGCT
  CATCGACTTCGGTTCGGGTGCGCTGCTCAAGGACACGGTCTACACCGACTTCGACGGCACCCGAGTGTAC
  AGCCCCCCGGAGTGGATCCGCTACCACCGCTACCACGGGCGCTCGGCCACCGTGTGGTCGCTGGGCGTGC
  TTCTCTACGATATGGTGTGTGGGGACATCCCCTTCGAGCAGGACGAGGAGATCCTCCGAGGCCGCCTGCT
  CTTCCGGAGGAGGGTCTCTCCAGAGTGCCAGCAGCTGATCCGGTGGTGCCTGTCCCTGCGGCCCTCAGAG
  CGGCCGTCGCTGGATCAGATTGCGGCCCATCCCTGGATGCTGGGGGCTGACGGGGGCGCCCCGGAGAGCT
  GTGACCTGCGGCTGTGCACCCTCGACCCTGATGACGTGGCCAGCACCACGTCCAGCAGCGAGAGCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212690 representing NM_001001852
  Red=Cloning site Green=Tags(s)
  
  MLLSKFGSLAHLCGPGGVDHLPVKILQPAKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVKH
  VVKERVTEWGSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERG
  ALDEPLARRFFAQVLAAVRHCHSCGVVHRDIKDENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVY
  SPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSE
  RPSLDQIAAHPWMLGADGGAPESCDLRLCTLDPDDVASTTSSSESL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001852
• ORF Size: 978 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001001852.2, NP_001001852.1
• RefSeq Size: 2392
• RefSeq ORF: 981
• Locus ID: 415116
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. [provided by RefSeq, Jun 2012]