PSMD13 (NM_175932) Human Tagged ORF Clone
CAT#: RC213020
PSMD13 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (PSMD13), transcript variant 2
"NM_175932" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PSMD13 |
Synonyms | HSPC027; p40.5; Rpn9; S11 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213020 representing NM_175932
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGACGTACCGGGCTTCCTACAGCAGAGCCAGAACTCCGGGCCCGGGCAGCCCGCTGTGTGGCACC GTCTGGAGGAGCTCTACACGAAGAACTTTATGAAAACTTTATCAGTGAATTTGAACACAGGTAAAAGCCT CTCATCCGTTTTTCACTTTGAAAATGAGTGCATTGATGCTCGGCGGTGCTCAAAGGCTGGTGGCTTTTAT TTCAGGGTGAACCCTTTGTCCCTCGTGGAAATCATTCTTCATGTAGTTAGACAGATGACTGATCCTAATG TGGCTCTTACTTTTCTGGAAAAGACTCGTGAGAAGGTGAAAAGTAGTGATGAGGCAGTGATCCTGTGTAA AACAGCAATTGGAGCTCTAAAATTAAACATCGGGGACCTACAGGTTACAAAGGAAACAATTGAAGATGTT GAAGAAATGCTCAACAACCTTCCTGGTGTGACATCGGTTCACAGTCGTTTCTATGATCTCTCCAGTAAAT ACTATCAAACAATCGGAAACCACGCGTCCTACTACAAAGATGCTCTGCGGTTTTTGGGCTGTGTTGACAT CAAGGATCTACCAGTGTCTGAGCAGCAGGAGAGAGCCTTCACGCTGGGGCTAGCAGGACTTCTCGGCGAG GGAGTTTTTAACTTTGGAGAACTCCTCATGCACCCTGTGCTGGAGTCCCTGAGGAATACTGACCGGCAGT GGCTGATTGACACCCTCTATGCCTTCAACAGTGGCAACGTAGAGCGGTTCCAGACTCTGAAGACTGCCTG GGGCCAGCAGCCTGATTTAGCAGCTAATGAAGCCCAGCTTCTGAGGAAAATTCAGTTGTTGTGCCTCATG GAGATGACTTTCACACGACCTGCCAATCACAGACAACTCACTTTTGAAGAAATTGCCAAAAGTGCTAAAA TCACAGTGAATGAGGTGGAGCTTCTGGTGATGAAGGCCCTTTCGGTGGGGCTGGTGAAAGGCAGTATAGA CGAGGTGGACAAACGAGTCCACATGACCTGGGTGCAGCCCCGAGTGTTGGATTTGCAACAGATCAAGGGA ATGAAGGACCGCCTGGAGTTCTGGTGCACGGATGTGAAGAGCATGGAGATGCTGGTGGAGCACCAGGCCC ATGACATCCTCACC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC213020 representing NM_175932
Red=Cloning site Green=Tags(s) MKDVPGFLQQSQNSGPGQPAVWHRLEELYTKNFMKTLSVNLNTGKSLSSVFHFENECIDARRCSKAGGFY FRVNPLSLVEIILHVVRQMTDPNVALTFLEKTREKVKSSDEAVILCKTAIGALKLNIGDLQVTKETIEDV EEMLNNLPGVTSVHSRFYDLSSKYYQTIGNHASYYKDALRFLGCVDIKDLPVSEQQERAFTLGLAGLLGE GVFNFGELLMHPVLESLRNTDRQWLIDTLYAFNSGNVERFQTLKTAWGQQPDLAANEAQLLRKIQLLCLM EMTFTRPANHRQLTFEEIAKSAKITVNEVELLVMKALSVGLVKGSIDEVDKRVHMTWVQPRVLDLQQIKG MKDRLEFWCTDVKSMEMLVEHQAHDILT SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_175932 |
ORF Size | 1134 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_175932.2, NP_787128.2 |
RefSeq Size | 1763 bp |
RefSeq ORF | 1137 bp |
Locus ID | 5719 |
Cytogenetics | 11p15.5 |
Protein Pathways | Proteasome |
MW | 42.8 kDa |
Gene Summary | 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC317607 | PSMD13 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (PSMD13), transcript variant 2 |
USD 650.00 |
|
RG213020 | PSMD13 (GFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (PSMD13), transcript variant 2 |
USD 460.00 |
|
RC213020L3 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (PSMD13), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC213020L4 | Lenti ORF clone of Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 (PSMD13), transcript variant 2, mGFP tagged |
USD 620.00 |
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