WFIKKN2 (NM_175575) Human Tagged ORF Clone

CAT#: RC213140

WFIKKN2 (Myc-DDK-tagged)-Human WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 (WFIKKN2)

Product Images

Western blot validation of overexpression lysate (Cat# LY406280) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213140 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified WFIKKN2 protein (Cat# TP313140). The protein was produced from HEK293T cells transfected with WFIKKN2 cDNA clone (Cat# RC213140) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WFIKKN2
• Synonyms: GASP-1; hGASP-1; WFDC20B; WFIKKNRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213140 representing NM_175575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGCCCCAAGGTGTCGCCGGTTCTGGTCTCGCTGGGAGCAGGTGGCAGCGCTGCTGCTGCTGCTGC
  TACTGCTCGGGGTGCCCCCGCGAAGCCTGGCGCTGCCGCCCATCCGCTATTCCCACGCCGGCATCTGCCC
  CAACGACATGAATCCCAACCTCTGGGTGGACGCACAGAGCACCTGCAGGCGGGAGTGTGAGACGGACCAG
  GAGTGTGAGACCTATGAGAAGTGCTGCCCCAACGTATGTGGGACCAAGAGCTGCGTGGCGGCCCGCTACA
  TGGACGTGAAAGGGAAGAAGGGCCCAGTGGGCATGCCCAAGGAGGCCACATGTGACCACTTCATGTGTCT
  GCAGCAGGGCTCTGAGTGTGACATCTGGGATGGCCAGCCCGTGTGTAAGTGCAAAGACCGCTGTGAGAAG
  GAGCCCAGCTTTACCTGCGCCTCGGACGGCCTCACCTACTATAACCGCTGCTACATGGATGCCGAGGCCT
  GCTCCAAAGGCATCACACTGGCCGTTGTAACCTGCCGCTATCACTTCACCTGGCCCAACACCAGCCCCCC
  ACCACCTGAGACCACCATGCACCCCACCACAGCCTCCCCAGAGACCCCTGAGCTGGACATGGCGGCCCCT
  GCGCTGCTCAACAACCCTGTGCACCAGTCGGTCACCATGGGTGAGACAGTGAGCTTCCTCTGTGATGTGG
  TGGGCCGGCCCCGGCCTGAGATCACCTGGGAGAAGCAGTTGGAGGATCGGGAGAATGTGGTCATGCGGCC
  CAACCATGTGCGTGGCAACGTGGTGGTCACCAACATTGCCCAGCTGGTCATCTATAACGCCCAGCTGCAG
  GATGCTGGGATCTACACCTGCACGGCCCGGAACGTGGCTGGGGTCCTGAGGGCTGATTTCCCGCTGTCGG
  TGGTCAGGGGTCATCAGGCTGCAGCCACCTCAGAGAGCAGCCCCAATGGCACGGCTTTCCCGGCGGCCGA
  GTGCCTGAAGCCCCCAGACAGTGAGGACTGTGGCGAAGAGCAGACCCGCTGGCACTTCGATGCCCAGGCC
  AACAACTGCCTGACCTTCACCTTCGGCCACTGCCACCGTAACCTCAACCACTTTGAGACCTATGAGGCCT
  GCATGCTGGCCTGCATGAGCGGGCCGCTGGCCGCGTGCAGCCTGCCCGCCCTGCAGGGGCCCTGCAAAGC
  CTACGCGCCTCGCTGGGCTTACAACAGCCAGACGGGCCAGTGCCAGTCCTTTGTCTATGGTGGCTGCGAG
  GGCAATGGCAACAACTTTGAGAGCCGTGAGGCCTGTGAGGAGTCGTGCCCCTTCCCCAGGGGGAACCAGC
  GCTGTCGGGCCTGCAAGCCTCGGCAGAAGCTCGTTACCAGCTTCTGTCGCAGCGACTTTGTCATCCTGGG
  CCGAGTCTCTGAGCTGACCGAGGAGCCTGACTCGGGCCGCGCCCTGGTGACTGTGGATGAGGTCCTAAAG
  GATGAGAAAATGGGCCTCAAGTTCCTGGGCCAGGAGCCATTGGAGGTCACTCTGCTTCACGTGGACTGGG
  CATGCCCCTGCCCCAACGTGACCGTGAGCGAGATGCCGCTCATCATCATGGGGGAGGTGGACGGCGGCAT
  GGCCATGCTGCGCCCCGATAGCTTTGTGGGCGCATCGAGTGCCCGCCGGGTCAGGAAGCTTCGTGAGGTC
  ATGCACAAGAAGACCTGTGACGTCCTCAAGGAGTTTCTTGGCTTGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213140 representing NM_175575
  Red=Cloning site Green=Tags(s)
  
  MWAPRCRRFWSRWEQVAALLLLLLLLGVPPRSLALPPIRYSHAGICPNDMNPNLWVDAQSTCRRECETDQ
  ECETYEKCCPNVCGTKSCVAARYMDVKGKKGPVGMPKEATCDHFMCLQQGSECDIWDGQPVCKCKDRCEK
  EPSFTCASDGLTYYNRCYMDAEACSKGITLAVVTCRYHFTWPNTSPPPPETTMHPTTASPETPELDMAAP
  ALLNNPVHQSVTMGETVSFLCDVVGRPRPEITWEKQLEDRENVVMRPNHVRGNVVVTNIAQLVIYNAQLQ
  DAGIYTCTARNVAGVLRADFPLSVVRGHQAAATSESSPNGTAFPAAECLKPPDSEDCGEEQTRWHFDAQA
  NNCLTFTFGHCHRNLNHFETYEACMLACMSGPLAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCE
  GNGNNFESREACEESCPFPRGNQRCRACKPRQKLVTSFCRSDFVILGRVSELTEEPDSGRALVTVDEVLK
  DEKMGLKFLGQEPLEVTLLHVDWACPCPNVTVSEMPLIIMGEVDGGMAMLRPDSFVGASSARRVRKLREV
  MHKKTCDVLKEFLGLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175575
• ORF Size: 1728 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175575.1, NM_175575.2, NM_175575.3, NM_175575.4, NM_175575.5, NP_783165.1
• RefSeq Size: 3534
• RefSeq ORF: 1731
• Locus ID: 124857
• Protein Families: Transmembrane
• MW: 63.8 kDa
• Gene Summary: The WFIKKN1 protein contains a WAP domain, follistatin domain, immunoglobulin domain, two tandem Kunitz domains, and an NTR domain. This gene encodes a WFIKKN1-related protein which has the same domain organization as the WFIKKN1 protein. The WAP-type, follistatin type, Kunitz-type, and NTR-type protease inhibitory domains may control the action of multiple types of proteases. [provided by RefSeq, Jul 2008]