CLNS1A (NM_001293) Human Tagged ORF Clone

CAT#: RC213169

CLNS1A (Myc-DDK-tagged)-Human chloride channel, nucleotide-sensitive, 1A (CLNS1A)

Product Images

Western blot validation of overexpression lysate (Cat# LY420002) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213169 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLNS1A
• Synonyms: CLCI; CLNS1B; ICln
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213169 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCCTCAAAAGTTTCCCGCCGCCTGGGCCAGCGGAGGGGCTCCTGCGGCAGCAGCCAGACACTG
  AGGCTGTGCTGAACGGGAAGGGCCTCGGCACTGGTACCCTTTACATCGCTGAGAGCCGCCTGTCTTGGTT
  AGATGGCTCTGGATTAGGATTCTCACTGGAATACCCCACCATTAGTTTACATGCATTATCCAGGGACCGA
  AGTGACTGTCTAGGAGAGCATTTGTATGTTATGGTGAATGCCAAATTTGAAGAAGAATCAAAAGAACCTG
  TTGCTGATGAAGAAGAGGAAGACAGTGATGATGATGTTGAACCTATTACTGAATTTAGATTTGTGCCTAG
  TGATAAATCAGCGTTGGAGGCAATGTTCACTGCAATGTGCGAATGCCAGGCCTTGCATCCAGATCCTGAG
  GATGAGGATTCAGATGACTACGATGGAGAAGAATATGATGTGGAAGCACATGAACAAGGACAGGGGGACA
  TCCCTACATTTTACACCTATGAAGAAGGATTATCCCATCTAACAGCAGAAGGCCAAGCCACACTGGAGAG
  ATTAGAAGGAATGCTTTCTCAGTCTGTGAGCAGCCAGTATAATATGGCTGGGGTCAGGACAGAAGATTCA
  ATAAGAGATTATGAAGATGGGATGGAGGTGGATACCACACCAACAGTTGCTGGACAGTTTGAGGATGCAG
  ATGTTGATCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213169 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFLKSFPPPGPAEGLLRQQPDTEAVLNGKGLGTGTLYIAESRLSWLDGSGLGFSLEYPTISLHALSRDR
  SDCLGEHLYVMVNAKFEEESKEPVADEEEEDSDDDVEPITEFRFVPSDKSALEAMFTAMCECQALHPDPE
  DEDSDDYDGEEYDVEAHEQGQGDIPTFYTYEEGLSHLTAEGQATLERLEGMLSQSVSSQYNMAGVRTEDS
  IRDYEDGMEVDTTPTVAGQFEDADVDH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293
• ORF Size: 711 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293.1, NM_001293.2, NP_001284.1
• RefSeq Size: 1378 bp
• RefSeq ORF: 714 bp
• Locus ID: 1207
• Cytogenetics: 11q14.1
• Domains: ICln_channel
• Protein Families: Ion Channels: Other
• MW: 26.2 kDa
• Gene Summary: 'This gene encodes a protein that functions in multiple regulatory pathways. The encoded protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator. Pseudogenes of this gene are found on chromosomes 1, 4 and 6. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]'