TPCN1 (NM_017901) Human Tagged ORF Clone

CAT#: RC213190

TPCN1 (Myc-DDK-tagged)-Human two pore segment channel 1 (TPCN1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TPCN1
• Synonyms: TPC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213190 representing NM_017901
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGAGTTTGGATGACGACGTGCCGCTCATCCTGACCTTGGATGAGGGTGGCAGTGCCCCACTGG
  CTCCCTCCAACGGCCTGGGCCAAGAAGAGCTACCTAGCAAAAATGGCGGCAGCTATGCCATCCACGACTC
  CCAGGCCCCCAGTCTCAGCTCTGGGGGTGAGAGTTCCCCCTCCAGCCCCGCACACAACTGGGAGATGAAT
  TACCAAGAGGCAGCAATCTACCTCCAGGAAGGCGAGAACAACGACAAGTTCTTCACCCACCCCAAGGATG
  CCAAGGCGCTGGCGGCCTACCTCTTTGCACACAATCACCTCTTCTACCTGATGGAGCTGGCCACGGCCCT
  GCTGCTGCTGCTGCTCTCCCTGTGCGAGGCCCCCGCCGTCCCCGCACTCCGGCTTGGCATCTATGTCCAC
  GCCACCCTGGAGCTGTTTGCCCTGATGGTGGTAGTGTTTGAACTCTGCATGAAGTTACGCTGGCTGGGCC
  TCCACACCTTCATCCGGCACAAGCGGACCATGGTCAAGACCTCGGTGCTGGTGGTGCAGTTTGTCGAGGC
  CATCGTGGTGTTGGTACGGCAGATGTCCCATGTGCGGGTGACCCGAGCACTGCGCTGCATTTTCCTGGTG
  GACTGTCGGTATTGCGGTGGCGTCCGGCGCAACCTGCGGCAGATCTTCCAGTCCCTGCCGCCCTTCATGG
  ACATCCTCCTGCTGCTGCTGTTCTTCATGATCATCTTTGCCATCCTCGGTTTCTACTTGTTCTCCCCTAA
  CCCTTCAGACCCCTACTTCAGCACCCTGGAGAACAGCATCGTCAGTCTGTTTGTCCTTCTGACCACAGCC
  AATTTCCCAGATGTGATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGCGTCTTCTTCATCGTGTACC
  TCTCCATCGAGCTGTATTTCATCATGAACCTGCTTCTGGCTGTGGTGTTCGACACCTTCAATGACATTGA
  GAAACGCAAGTTCAAGTCTTTGCTACTGCACAAGCGAACCGCTATCCAGCATGCCTACCGCCTGCTCATC
  AGCCAGAGGAGGCCTGCCGGCATCTCCTACAGGCAGTTTGAAGGCCTCATGCGCTTCTACAAGCCCCGGA
  TGAGTGCCAGGGAGCGCTATCTTACCTTCAAGGCCCTGAATCAGAACAACACACCCCTGCTCAGCCTAAA
  GGACTTTTACGATATCTACGAAGTTGCTGCTTTGAAGTGGAAGGCCAAGAAAAACAGAGAGCACTGGTTT
  GATGAGCTTCCCAGGACGGCGCTCCTCATCTTCAAAGGTATTAATATCCTTGTGAAGTCCAAGGCCTTCC
  AGTATTTCATGTACTTGGTGGTGGCAGTCAACGGGGTCTGGATCCTCGTGGAGACATTTATGCTGAAAGG
  TGGGAACTTCTTCTCCAAGCACGTGCCCTGGAGTTACCTCGTCTTTCTAACTATCTATGGGGTGGAGCTG
  TTCCTGAAGGTTGCCGGCCTGGGCCCTGTGGAGTACTTGTCTTCCGGATGGAACTTGTTTGACTTCTCCG
  TGACAGTGTTCGCCTTCCTGGGACTGCTGGCGCTGGCCCTCAACATGGAGCCCTTCTATTTCATCGTGGT
  CCTGCGCCCCCTCCAGCTGCTGAGGTTGTTTAAGTTGAAGGAGCGCTACCGCAACGTGCTGGACACCATG
  TTCGAGCTGCTGCCCCGGATGGCCAGCCTGGGCCTCACCCTGCTCATCTTTTACTACTCCTTCGCCATCG
  TGGGCATGGAGTTCTTCTGCGGGATCGTCTTCCCCAACTGCTGCAACACGAGTACAGTGGCAGATGCCTA
  CCGCTGGCGCAACCACACCGTGGGCAACAGGACCGTGGTGGAGGAAGGCTACTATTATCTCAATAATTTT
  GACAACATCCTCAACAGCTTTGTGACCCTGTTTGAGCTCACAGTTGTCAACAACTGGTACATCATCATGG
  AAGGCGTCACCTCTCAGACCTCCCACTGGAGCCGCCTCTACTTCATGACCTTTTACATTGTGACCATGGT
  GGTGATGACGATCATTGTCGCCTTTATCCTCGAGGCCTTCGTCTTCCGAATGAACTACAGCCGCAAGAAC
  CAGGACTCGGAAGTTGATGGTGGCATCACCCTTGAGAAGGAAATCTCCAAAGAAGAGCTGGTTGCCGTCC
  TGGAGCTCTACCGGGAGGCACGGGGGGCCTCCTCGGATGTCACCAGGCTGCTGGAGACCCTCTCCCAGAT
  GGAGAGATACCAGCAACATTCCATGGTGTTTCTGGGACGGCGATCAAGGACCAAGAGCGACCTGAGCCTG
  AAGATGTACCAGGAGGAGATCCAGGAGTGGTATGAGGAGCATGCCAGGGAGCAAGAGCAGCAGCGACAAC
  TCAGCAGCAGTGCAGCCCCCGCCGCCCAGCAGCCCCCAGGCAGCCGCCAGCGCTCCCAGACCGTTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213190 representing NM_017901
  Red=Cloning site Green=Tags(s)
  
  MAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMN
  YQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVH
  ATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLV
  DCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTA
  NFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLI
  SQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWF
  DELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVEL
  FLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTM
  FELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNF
  DNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKN
  QDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSL
  KMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_017901
• ORF Size: 2448 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017901.3, NM_017901.4, NM_017901.5, NP_060371.2
• RefSeq Size: 5396
• RefSeq ORF: 2451
• Locus ID: 53373
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 94.1 kDa
• Gene Summary: Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]). [supplied by OMIM, Mar 2008]