Nardilysin (NRDC) (NM_002525) Human Tagged ORF Clone

CAT#: RC213239

NRD1 (Myc-DDK-tagged)-Human nardilysin (N-arginine dibasic convertase) (NRD1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY419269) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213239 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NRDC protein (Cat# TP313239). The protein was produced from HEK293T cells transfected with NRDC cDNA clone (Cat# RC213239) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NRDC
• Synonyms: hNRD1; hNRD2; NRD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213239 representing NM_002525
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGAGAGTCACTGTTGCTGCAGTCTGTGCCACCCGGAGGAAGTTGTGTGAGGCCGGGCGGGACG
  TCGCGGCGCTCTGGGGAATCGAAACGCGGGGTCGGTGCGAAGACTCTGCTGCTGCCAGACCCTTTCCTAT
  TCTGGCCATGCCTGGAAGGAACAAGGCGAAGTCTACCTGCAGCTGCCCTGACCTGCAGCCCAATGGACAG
  GATCTGGGCGAGAACAGCCGGGTTGCCCGTCTAGGAGCGGATGAATCTGAGGAAGAGGGACGGAGGGGGT
  CTCTCAGTAATGCTGGGGACCCTGAGATCGTCAAGTCTCCCAGCGACCCCAAGCAATACCGATACATCAA
  ATTACAGAATGGCCTACAGGCACTTCTGATTTCAGACCTAAGTAATATGGAAGGTAAAACAGGAAATACA
  ACAGATGATGAAGAAGAAGAGGAGGTGGAGGAAGAAGAAGAAGATGATGATGAAGATTCTGGAGCTGAAA
  TAGAAGATGACGATGAAGAGGGTTTTGATGATGAAGATGAGTTTGATGATGAACATGATGATGATCTTGA
  TACTGAGGATAATGAATTGGAAGAATTAGAAGAGAGAGCAGAAGCTAGAAAAAAAACTACTGAAAAACAG
  CAATTGCAGAGCCTGTTTTTGCTGTGGTCAAAGCTGACTGATAGACTGTGGTTTAAGTCAACTTATTCAA
  AAATGTCTTCAACCCTGCTGGTCGAGACAAGAAATCTTTATGGGGTAGTTGGAGCTGAAAGCAGGTCTGC
  ACCTGTTCAGCATTTGGCAGGATGGCAAGCGGAGGAGCAGCAGGGTGAAACTGACACAGTTCTGTCTGCA
  GCGGCTCTTTGTGTTGGAGTTGGGAGTTTCGCTGATCCAGATGACCTGCCGGGGCTGGCACACTTTTTGG
  AGCACATGGTATTCATGGGTAGTTTGAAATATCCAGATGAGAATGGATTTGATGCCTTCCTGAAGAAGCA
  TGGGGGTAGTGATAATGCCTCAACTGATTGTGAACGCACTGTCTTTCAGTTTGATGTCCAGAGGAAGTAC
  TTCAAGGAAGCTCTTGATAGATGGGCGCAGTTCTTCATCCACCCACTAATGATCAGAGATGCAATTGACC
  GTGAAGTTGAAGCTGTTGATAGTGAATATCAACTTGCAAGGCCTTCTGATGCAAACAGAAAGGAAATGTT
  GTTTGGAAGCCTTGCTAGACCTGGCCATCCTATGGGAAAATTTTTTTGGGGAAATGCTGAGACGCTCAAG
  CATGAGCCAAGAAAGAATAATATTGATACACATGCTAGATTGAGAGAATTCTGGATGCGTTACTACTCTT
  CTCATTACATGACTTTAGTGGTTCAATCCAAAGAAACACTGGATACTTTGGAAAAGTGGGTGACTGAAAT
  CTTCTCTCAGATACCAAACAATGGGTTACCCAGACCAAACTTTGGCCATTTAACGGATCCATTTGACACA
  CCAGCATTTAACAAACTTTATAGAGTTGTTCCAATCAGAAAAATTCATGCTCTGACCATCACATGGGCAC
  TTCCTCCTCAACAGCAACATTACAGGGTGAAGCCACTTCATTATATATCCTGGCTGGTTGGACATGAAGG
  CAAAGGCAGCATTCTTTCTTTCCTTAGGAAAAAATGCTGGGCTCTTGCACTGTTTGGTGGAAATGGTGAG
  ACAGGATTTGAGCAAAATTCTACTTATTCAGTGTTCAGCATTTCTATTACATTGACTGATGAGGGTTATG
  AACATTTTTATGAGGTTGCTTACACTGTCTTTCTGTATTTAAAAATGCTGCAGAAGCTAGGCCCAGAAAA
  AAGAATTTTTGAAGAGATTCGGAAAATTGAGGATAATGAATTTCATTACCAAGAACAGACAGATCCAGTT
  GAGTATGTGGAAAACATGTGTGAGAACATGCAGCTGTACCCATTGCAGGACATTCTCACTGGAGATCAGC
  TTCTTTTTGAATACAAGCCAGAAGTCATTGGTGAAGCCTTGAATCAGCTAGTTCCTCAAAAAGCAAATCT
  TGTTTTACTGTCTGGTGCTAATGAGGGAAAATGTGACCTCAAGGAGAAATGGTTTGGAACTCAATATAGT
  ATAGAAGATATTGAAAACTCTTGGGCTGAACTGTGGAATAGTAATTTCGAATTAAATCCAGATCTTCATC
  TTCCAGCTGAAAACAAGTACATAGCCACGGACTTTACGTTGAAGGCTTTCGATTGCCCGGAAACAGAATA
  CCCAGTTAAAATTGTGAATACTCCACAAGGTTGCCTGTGGTATAAGAAAGACAACAAATTCAAAATCCCC
  AAAGCATATATACGTTTCCATCTAATTTCACCGTTGATACAGAAATCTGCAGCAAATGTGGTCCTCTTTG
  ATATCTTTGTCAATATCCTTACGCATAACCTTGCGGAACCAGCTTATGAAGCAGATGTGGCACAGCTGGA
  GTATAAACTGGCAGCTGGAGAACATGGTTTAATTATTCGAGTGAAAGGATTTAACCACAAACTACCTCTA
  CTGTTTCAGCTCATTATTGACTACTTAGCTGAGTTCAATTCCACACCAGCTGTCTTTACAATGATAACTG
  AGCAGTTGAAGAAGACCTACTTTAACATCCTCATCAAGCCTGAGACTTTGGCCAAAGATGTACGGCTTTT
  AATCTTGGAATATGCCCGTTGGTCTATGATTGACAAGTACCAGGCTTTGATGGACGGCCTTTCCCTTGAG
  TCTCTGCTGAGCTTCGTCAAAGAATTCAAATCCCAGCTCTTTGTGGAGGGCCTGGTACAAGGGAATGTCA
  CAAGCACAGAATCTATGGATTTCCTGAAATATGTTGTTGACAAACTAAACTTCAAGCCTCTGGAGCAGGA
  GATGCCTGTGCAGTTCCAGGTGGTAGAGCTGCCCAGTGGCCACCATCTATGCAAAGTGAAAGCTCTGAAC
  AAGGGTGATGCCAACTCTGAAGTCACTGTGTACTACCAGTCAGGTACCAGGAGTCTAAGAGAATATACGC
  TTATGGAGCTGCTTGTGATGCACATGGAAGAACCTTGTTTTGACTTCCTTCGAACCAAGCAGACCCTTGG
  GTACCATGTCTACCCTACCTGTAGGAACACATCCGGGATTCTAGGATTTTCTGTCACTGTGGGGACTCAG
  GCAACCAAATACAATTCTGAAGTTGTTGATAAGAAGATAGAAGAGTTTCTTTCTAGCTTTGAGGAGAAGA
  TTGAGAACCTCACTGAAGAGGCATTCAACACCCAGGTCACAGCTCTCATCAAGCTGAAGGAGTGTGAGGA
  TACCCACCTTGGGGAGGAGGTGGATAGGAACTGGAATGAAGTGGTTACACAGCAGTACCTCTTTGACCGC
  CTTGCCCACGAGATTGAAGCACTGAAGTCATTCTCAAAATCAGACCTGGTCAACTGGTTCAAGGCTCATA
  GAGGGCCAGGAAGTAAAATGCTCAGCGTTCATGTTGTTGGGTATGGGAAGTATGAACTGGAAGAGGATGG
  ATCCCCTTCTAGTGAGGATTCAAATTCTTCTTGTGAAGTGATGCAGCTGACCTACCTGCCAACCTCTCCT
  CTGCTGGCAGATTGTATCATCCCCATTACTGATATCAGGGCTTTCACAACAACACTCAACCTTCTCCCCT
  ACCATAAAATAGTCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213239 representing NM_002525
  Red=Cloning site Green=Tags(s)
  
  MLRRVTVAAVCATRRKLCEAGRDVAALWGIETRGRCEDSAAARPFPILAMPGRNKAKSTCSCPDLQPNGQ
  DLGENSRVARLGADESEEEGRRGSLSNAGDPEIVKSPSDPKQYRYIKLQNGLQALLISDLSNMEGKTGNT
  TDDEEEEEVEEEEEDDDEDSGAEIEDDDEEGFDDEDEFDDEHDDDLDTEDNELEELEERAEARKKTTEKQ
  QLQSLFLLWSKLTDRLWFKSTYSKMSSTLLVETRNLYGVVGAESRSAPVQHLAGWQAEEQQGETDTVLSA
  AALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKY
  FKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLK
  HEPRKNNIDTHARLREFWMRYYSSHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPRPNFGHLTDPFDT
  PAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSFLRKKCWALALFGGNGE
  TGFEQNSTYSVFSISITLTDEGYEHFYEVAYTVFLYLKMLQKLGPEKRIFEEIRKIEDNEFHYQEQTDPV
  EYVENMCENMQLYPLQDILTGDQLLFEYKPEVIGEALNQLVPQKANLVLLSGANEGKCDLKEKWFGTQYS
  IEDIENSWAELWNSNFELNPDLHLPAENKYIATDFTLKAFDCPETEYPVKIVNTPQGCLWYKKDNKFKIP
  KAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLAAGEHGLIIRVKGFNHKLPL
  LFQLIIDYLAEFNSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYARWSMIDKYQALMDGLSLE
  SLLSFVKEFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFKPLEQEMPVQFQVVELPSGHHLCKVKALN
  KGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQ
  ATKYNSEVVDKKIEEFLSSFEEKIENLTEEAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDR
  LAHEIEALKSFSKSDLVNWFKAHRGPGSKMLSVHVVGYGKYELEEDGSPSSEDSNSSCEVMQLTYLPTSP
  LLADCIIPITDIRAFTTTLNLLPYHKIVK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002525
• ORF Size: 3657 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002525.1, NP_002516.1
• RefSeq Size: 3851 bp
• RefSeq ORF: 3660 bp
• Locus ID: 4898
• Cytogenetics: 1p32.3
• Domains: Peptidase_M16, Peptidase_M16_C
• Protein Families: Druggable Genome, Protease
• MW: 139.2 kDa
• Gene Summary: 'This gene encodes a zinc-dependent endopeptidase that cleaves peptide substrates at the N-terminus of arginine residues in dibasic moieties and is a member of the peptidase M16 family. This protein interacts with heparin-binding EGF-like growth factor and plays a role in cell migration and proliferation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]'