MOGT1 (MOGAT1) (NM_058165) Human Tagged ORF Clone

CAT#: RC213249

MOGAT1 (Myc-DDK-tagged)-Human monoacylglycerol O-acyltransferase 1 (MOGAT1)

Product Images

Western blot validation of overexpression lysate (Cat# LY408961) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213249 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MOGAT1
• Synonyms: DGAT2L; DGAT2L1; MGAT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213249 representing NM_058165
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTAGAGTTTGCACCGCTCAACATCCAGCTGGCGCGGCGGCTGCAGACGGTGGCCGTGCTGCAGT
  GGGTCCTGAAATACCTGCTGCTCGGGCCGATGTCCATTGGAATCACTGTGATGCTGATCATACACAACTA
  TTTGTTCCTTTACATCCCTTATTTGATGTGGCTTTACTTTGACTGGCATACCCCAGAGCGAGGAGGCAGG
  AGATCCAGCTGGATCAAAAATTGGACTCTTTGGAAACACTTTAAGGACTATTTTCCAATTCATCTTATCA
  AAACTCAAGATTTGGATCCAAGTCACAACTATATATTTGGGTTTCACCCCCATGGAATAATGGCAGTTGG
  AGCCTTTGGGAATTTTTCTGTAAATTATTCTGACTTCAAGGACCTGTTTCCTGGCTTTACTTCATATCTT
  CACGTGCTGCCACTTTGGTTCTGGTGTCCTGTCTTTCGAGAATATGTGATGAGTGTTGGGCTGGTTTCAG
  TTTCCAAGAAAAGTGTGTCCTACATGGTAAGCAAGGAGGGAGGTGGAAACATCTCTGTCATTGTCCTTGG
  GGGTGCAAAAGAATCACTGGATGCTCATCCTGGAAAGTTCACTCTGTTCATCCGCCAGCGGAAAGGATTT
  GTTAAAATTGCTTTGACCCATGGCGCCTCTCTGGTCCCAGTGGTTTCTTTTGGTGAAAATGAACTGTTTA
  AACAAACTGACAACCCTGAAGGATCATGGATTAGAACTGTTCAGAATAAACTGCAGAAGATCATGGGGTT
  TGCTTTGCCCCTGTTTCATGCCAGGGGAGTTTTTCAGTACAATTTTGGCCTAATGACCTATAGGAAAGCC
  ATCCACACTGTTGTTGGCCGCCCGATCCCTGTTCGTCAGACTCTGAACCCGACCCAGGAGCAGATTGAGG
  AGTTACATCAGACCTATATGGAGGAACTTAGGAAATTGTTTGAGGAACACAAAGGAAAGTATGGCATTCC
  AGAGCACGAGACTCTTGTTTTAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213249 representing NM_058165
  Red=Cloning site Green=Tags(s)
  
  MKVEFAPLNIQLARRLQTVAVLQWVLKYLLLGPMSIGITVMLIIHNYLFLYIPYLMWLYFDWHTPERGGR
  RSSWIKNWTLWKHFKDYFPIHLIKTQDLDPSHNYIFGFHPHGIMAVGAFGNFSVNYSDFKDLFPGFTSYL
  HVLPLWFWCPVFREYVMSVGLVSVSKKSVSYMVSKEGGGNISVIVLGGAKESLDAHPGKFTLFIRQRKGF
  VKIALTHGASLVPVVSFGENELFKQTDNPEGSWIRTVQNKLQKIMGFALPLFHARGVFQYNFGLMTYRKA
  IHTVVGRPIPVRQTLNPTQEQIEELHQTYMEELRKLFEEHKGKYGIPEHETLVLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_058165
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_058165.2, NP_477513.2
• RefSeq Size: 1096
• RefSeq ORF: 1008
• Locus ID: 116255
• Protein Families: Transmembrane
• MW: 38.6 kDa
• Gene Summary: Acyl-CoA:monoacylglycerol acyltransferase (MOGAT; EC 2.3.1.22) catalyzes the synthesis of diacylglycerols, the precursor of physiologically important lipids such as triacylglycerol and phospholipids (Yen et al., 2002 [PubMed 12077311]). [supplied by OMIM, Mar 2008]