CLCC1 (NM_001048210) Human Tagged ORF Clone

CAT#: RC213260

CLCC1 (Myc-DDK-tagged)-Human chloride channel CLIC-like 1 (CLCC1), transcript variant 1

Product Images

Coomassie blue staining of purified CLCC1 protein (Cat# TP313260). The protein was produced from HEK293T cells transfected with CLCC1 cDNA clone (Cat# RC213260) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLCC1
• Synonyms: MCLC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213260 representing NM_001048210
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGTTCTTTGCTCCTTTGTGAATGTCTGTTGCTGGTAGCTGGTTATGCTCATGATGATGACTGGA
  TTGACCCCACAGACATGCTTAACTATGATGCTGCTTCAGGAACAATGAGAAAATCTCAGGCAAAATATGG
  TATTTCAGGGGAAAAGGATGTCAGTCCTGACTTGTCATGTGCTGATGAAATATCAGAATGTTATCACAAA
  CTTGATTCTTTAACTTATAAGATTGATGAGTGTGAAAAGAAAAAGAGGGAAGACTATGAAAGTCAAAGCA
  ATCCTGTTTTTAGGAGATACTTAAATAAGATTTTAATTGAAGCTGGAAAGCTTGGACTTCCTGATGAAAA
  CAAAGGCGATATGCATTATGATGCTGAGATTATCCTTAAAAGAGAAACTTTGTTAGAAATACAGAAGTTT
  CTCAATGGAGAAGACTGGAAACCAGGTGCCTTGGATGATGCACTAAGTGATATTTTAATTAATTTTAAGT
  TTCATGATTTTGAAACATGGAAGTGGCGATTCGAAGATTCCTTTGGAGTGGATCCATATAATGTGTTAAT
  GGTACTTCTTTGTCTGCTCTGCATCGTGGTTTTAGTGGCTACCGAGCTGTGGACATATGTACGTTGGTAC
  ACTCAGTTGAGACGTGTTTTAATCATCAGCTTTCTGTTCAGTTTGGGATGGAATTGGATGTATTTATATA
  AGCTAGCTTTTGCACAGCATCAGGCTGAAGTCGCCAAGATGGAGCCATTAAACAATGTGTGTGCCAAAAA
  GATGGACTGGACTGGAAGTATCTGGGAATGGTTTAGAAGTTCATGGACCTATAAGGATGACCCATGCCAA
  AAATACTATGAGCTCTTACTAGTCAACCCTATTTGGTTGGTCCCACCAACAAAGGCACTTGCAGTTACAT
  TCACCACATTTGTAACGGAGCCATTGAAGCATATTGGAAAAGGAACTGGGGAATTTATTAAAGCACTCAT
  GAAGGAAATTCCAGCGCTGCTTCATCTTCCAGTGCTGATAATTATGGCATTAGCCATCCTGAGTTTCTGC
  TATGGTGCTGGAAAATCAGTTCATGTGCTGAGACATATAGGCGGTCCTGAGAGAGAACCTCCCCAGGCAC
  TTCGGCCACGGGATAGAAGACGGCAGGAGGAAATTGATTATAGACCTGATGGTGGAGCAGGTGATGCCGA
  TTTCCATTATAGGGGCCAAATGGGCCCCACTGAGCAAGGCCCTTATGCCAAAACGTATGAGGGTAGAAGA
  GAGATTTTGAGAGAGAGAGATGTTGACTTGAGATTTCAGACTGGCAACAAGAGCCCTGAAGTGCTCCGGG
  CATTTGATGTACCAGACGCAGAGGCACGAGAGCATCCCACGGTGGTACCCAGTCATAAATCACCTGTTTT
  GGATACAAAGCCCAAGGAGACAGGTGGAATCCTGGGGGAAGGCACACCGAAAGAAAGCAGTACTGAAAGC
  AGCCAGTCGGCCAAGCCTGTCTCTGGCCAAGACACATCAGGGAATACAGAAGGTTCACCCGCAGCGGAAA
  AGGCCCAGCTCAAGTCTGAAGCCGCAGGCAGCCCAGACCAAGGCAGCACATACAGCCCCGCAAGAGGTGT
  GGCTGGACCACGTGGACAGGATCCGGTCAGCAGCCCCTGTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213260 representing NM_001048210
  Red=Cloning site Green=Tags(s)
  
  MLCSLLLCECLLLVAGYAHDDDWIDPTDMLNYDAASGTMRKSQAKYGISGEKDVSPDLSCADEISECYHK
  LDSLTYKIDECEKKKREDYESQSNPVFRRYLNKILIEAGKLGLPDENKGDMHYDAEIILKRETLLEIQKF
  LNGEDWKPGALDDALSDILINFKFHDFETWKWRFEDSFGVDPYNVLMVLLCLLCIVVLVATELWTYVRWY
  TQLRRVLIISFLFSLGWNWMYLYKLAFAQHQAEVAKMEPLNNVCAKKMDWTGSIWEWFRSSWTYKDDPCQ
  KYYELLLVNPIWLVPPTKALAVTFTTFVTEPLKHIGKGTGEFIKALMKEIPALLHLPVLIIMALAILSFC
  YGAGKSVHVLRHIGGPEREPPQALRPRDRRRQEEIDYRPDGGAGDADFHYRGQMGPTEQGPYAKTYEGRR
  EILRERDVDLRFQTGNKSPEVLRAFDVPDAEAREHPTVVPSHKSPVLDTKPKETGGILGEGTPKESSTES
  SQSAKPVSGQDTSGNTEGSPAAEKAQLKSEAAGSPDQGSTYSPARGVAGPRGQDPVSSPCG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001048210
• ORF Size: 1653 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001048210.1, NM_001048210.2, NP_001041675.1
• RefSeq Size: 4825
• RefSeq ORF: 1656
• Locus ID: 23155
• Protein Families: Ion Channels: Other, Transmembrane
• MW: 61.8 kDa