ACSM4 (NM_001080454) Human Tagged ORF Clone

CAT#: RC213310

ACSM4 (Myc-DDK-tagged)-Human acyl-CoA synthetase medium-chain family member 4 (ACSM4)

Product Images

Western blot validation of overexpression lysate (Cat# LY420712) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213310 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ACSM4 protein (Cat# TP313310). The protein was produced from HEK293T cells transfected with ACSM4 cDNA clone (Cat# RC213310) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACSM4
• Synonyms: acyl-CoA synthetase medium-chain family member 4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213310 representing NM_001080454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTTTTTTCCGCTACCAGACATTTAGATTCATCTGGCTCACCAAGCCACCTGGCCGGCGCTTAC
  ACAAAGATCACCAGCTTTGGACGCCTCTGACTCTTGCTGACTTTGAAGCCATAAATCGCTGTAACAGGCC
  ATTGCCTAAAAACTTTAACTTTGCTGCAGATGTGCTGGACCAGTGGTCCCAAAAGGAGAAGACAGGGGAG
  AGACCAGCTAACCCAGCCCTGTGGTGGGTGAATGGCAAAGGGGATGAGGTAAAATGGAGCTTCAGAGAAC
  TGGGCTCCTTGTCCCGAAAAGCTGCCAACGTGCTCACCAAGCCCTGTGGCCTGCAGAGAGGAGACCGTTT
  GGCCGTGATTCTGCCCAGAATCCCTGAGTGGTGGCTGGTCAATGTAGCTTGCATACGAACAGGGATCATC
  TTCATGCCGGGAACAATCCAGCTGACAGCAAAAGACATCCTCTACCGGCTGCGAGCATCCAAGGCCAAGT
  GCATTGTGGCCAGTGAGGAGGTGGCCCCAGCGGTGGAGTCCATTGTATTGGAGTGTCCTGACCTTAAGAC
  AAAACTCCTGGTGTCTCCACAAAGCTGGAATGGGTGGCTCAGCTTCCAGGAGTTATTTCAATTCGCCTCT
  GAAGAGCACAGCTGTGTGGAAACAGGAAGTCAAGAACCAATGACCATTTATTTCACCAGTGGGACCACAG
  GCTTTCCTAAAATGGCCCAGCACTCTCAGAGCAGCCTCGGCATTGGGTTCACCCTCTGCGGAAGGTATTG
  GCTGGACTTGAAGTCCTCAGATATCATATGGAATATGTCTGACACGGGCTGGGTCAAGGCCGCCATTGGC
  AGTGTGTTTTCTTCCTGGCTGTGTGGAGCCTGTGTTTTTGTGCATCGAATGGCACAGTTTGACACTGACA
  CCTTCCTAGACACACTTACTACTTATCCCATCACGACCCTGTGCAGTCCTCCCACTGTGTACCGGATGCT
  CGTGCAAAAAGACCTTAAGAGATATAAATTCAAGAGTCTGCGGCACTGCTTGACCGGAGGGGAGCCACTC
  AACCCAGAAGTGCTGGAGCAGTGGAGGGTGCAAACTGGGCTGGAGCTATATGAGGGCTATGGACAGACGG
  AAGTGGGAATGATTTGTGCCAATCAGAAAGGCCAAGAAATTAAACCAGGTTCAATGGGGAAAGGAATGCT
  GCCCTATGATGTCCAGATTATAGATGAAAATGGCAATGTTCTACCACCTGGCAAAGAAGGGGAAATTGCC
  CTCAGACTCAAACCTACACGGCCCTTCTGTTTCTTCTCTAAATATGTGGACAATCCACAGAAAACTGCTG
  CCACGATAAGAGGAGATTTTTATGTCACTGGAGACAGAGGAGTGATGGACAGTGATGGGTATTTCTGGTT
  TGTCGGCAGAGCTGATGATGTCATTATATCCTCTGGGTACCGTATTGGGCCATTTGAAGTGGAGAGTGCA
  CTCATTGAGCATCCAGCAGTTGTTGAATCGGCTGTTGTCAGTAGTCCAGATCAAATCCGCGGAGAGGTGG
  TGAAAGCTTTTGTTGTCTTAGCTGCACCCTTTAAGTCCTACAACCCAGAGAAATTAACTCTTGAACTTCA
  GGATCATGTGAAAAAATCAACTGCACCTTACAAATATCCAAGAAAGGTGGAATTTGTTCAAGAACTCCCA
  AAGACAATCACTGGGAAAATCAAACGCAACGTTTTAAGAGACCAAGAATGGAGAGGAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213310 representing NM_001080454
  Red=Cloning site Green=Tags(s)
  
  MKIFFRYQTFRFIWLTKPPGRRLHKDHQLWTPLTLADFEAINRCNRPLPKNFNFAADVLDQWSQKEKTGE
  RPANPALWWVNGKGDEVKWSFRELGSLSRKAANVLTKPCGLQRGDRLAVILPRIPEWWLVNVACIRTGII
  FMPGTIQLTAKDILYRLRASKAKCIVASEEVAPAVESIVLECPDLKTKLLVSPQSWNGWLSFQELFQFAS
  EEHSCVETGSQEPMTIYFTSGTTGFPKMAQHSQSSLGIGFTLCGRYWLDLKSSDIIWNMSDTGWVKAAIG
  SVFSSWLCGACVFVHRMAQFDTDTFLDTLTTYPITTLCSPPTVYRMLVQKDLKRYKFKSLRHCLTGGEPL
  NPEVLEQWRVQTGLELYEGYGQTEVGMICANQKGQEIKPGSMGKGMLPYDVQIIDENGNVLPPGKEGEIA
  LRLKPTRPFCFFSKYVDNPQKTAATIRGDFYVTGDRGVMDSDGYFWFVGRADDVIISSGYRIGPFEVESA
  LIEHPAVVESAVVSSPDQIRGEVVKAFVVLAAPFKSYNPEKLTLELQDHVKKSTAPYKYPRKVEFVQELP
  KTITGKIKRNVLRDQEWRGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080454
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080454.1, NP_001073923.1
• RefSeq Size: 1743
• RefSeq ORF: 1743
• Locus ID: 341392
• Protein Pathways: Butanoate metabolism, Metabolic pathways
• MW: 65.5 kDa