THRAP3 (NM_005119) Human Tagged ORF Clone

CAT#: RC213342

THRAP3 (Myc-DDK-tagged)-Human thyroid hormone receptor associated protein 3 (THRAP3)

Product Images

Western blot validation of overexpression lysate (Cat# LY417509) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213342 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: THRAP3
• Synonyms: BCLAF2; TRAP150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213342 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAAAACAAACAAATCCAAGTCTGGATCTCGCTCTTCTCGCTCAAGATCTGCATCAAGATCTCGTT
  CTCGTTCATTTTCGAAGTCTCGGTCCCGAAGCCGATCTCTCTCTCGTTCAAGGAAGCGCAGGCTGAGTTC
  TAGGTCTCGTTCCAGATCATATTCTCCAGCTCATAACAGAGAAAGAAACCACCCAAGAGTATATCAGAAT
  CGGGATTTCCGAGGTCACAACAGAGGCTATAGAAGGCCCTATTATTTCCGTGGGCGTAACAGAGGCTTTT
  ATCCATGGGGCCAATATAACCGAGGAGGCTATGGAAACTACCGCTCAAATTGGCAGAATTACCGGCAAGC
  ATACAGTCCTCGTCGAGGCCGTTCAAGATCCCGGTCCCCAAAGAGAAGGTCCCCTTCACCAAGGTCCAGG
  AGCCATTCTAGAAACTCTGATAAGTCGTCTTCTGACCGGTCAAGGCGCTCCTCATCCTCCCGTTCTTCCT
  CCAACCATAGCCGAGTTGAATCTTCTAAGCGCAAGTCTGCAAAGGAGAAAAAGTCCTCTTCTAAGGATAG
  CCGGCCATCTCAGGCTGCCGGGGATAACCAGGGAGATGAGGCCAAGGAGCAGACATTCTCTGGAGGCACC
  TCTCAAGATACAAAAGCATCTGAGAGCTCGAAGCCATGGCCAGATGCCACCTACGGCACTGGTTCTGCAT
  CACGGGCCTCAGCAGTTTCTGAGCTGAGTCCTCGGGAGCGAAGCCCAGCTCTCAAAAGCCCCCTCCAGTC
  TGTGGTGGTGAGGCGGCGGTCACCCCGTCCTAGCCCCGTGCCAAAACCTAGTCCTCCACTTTCCAGCACA
  TCCCAGATGGGCTCAACTCTGCCGAGTGGTGCCGGGTATCAGTCTGGGACACACCAAGGTCAGTTCGACC
  ATGGTTCTGGGTCCCTGAGTCCATCCAAAAAGAGCCCTGTGGGTAAGAGTCCACCATCCACTGGCTCCAC
  ATATGGCTCATCTCAGAAGGAGGAGAGTGCTGCTTCAGGAGGAGCAGCCTATACAAAGAGGTATCTAGAA
  GAGCAGAAGACAGAGAATGGAAAAGATAAGGAACAGAAACAAACAAATACCGATAAAGAAAAAATAAAAG
  AGAAAGGGAGCTTCTCTGACACAGGCTTGGGTGATGGAAAAATGAAATCTGATTCTTTTGCTCCCAAAAC
  TGATTCTGAGAAGCCTTTTCGGGGCAGTCAGTCTCCCAAAAGGTATAAGCTCCGAGATGACTTTGAGAAG
  AAGATGGCTGACTTCCACAAGGAGGAGATGGATGATCAAGATAAGGACAAAGCTAAGGGAAGAAAGGAAT
  CTGAGTTTGATGATGAACCCAAATTTATGTCTAAAGTCATAGGTGCAAACAAAAACCAGGAGGAGGAGAA
  GTCAGGCAAATGGGAGGGCCTGGTATATGCACCTCCAGGGAAGGAAAAGCAGAGAAAAACAGAGGAGCTG
  GAGGAGGAGTCTTTCCCAGAGAGATCCAAAAAGGAAGATCGGGGCAAGAGAAGCGAAGGTGGGCACAGGG
  GCTTTGTGCCTGAGAAGAATTTCCGAGTGACTGCTTATAAAGCAGTCCAGGAGAAAAGCTCATCACCTCC
  CCCAAGAAAGACCTCTGAGAGCCGAGACAAGCTGGGAGCGAAAGGAGATTTTCCCACAGGAAAGTCTTCC
  TTTTCCATTACTCGAGAGGCACAGGTCAATGTCCGGATGGACTCTTTTGATGAGGACCTCGCACGACCCA
  GTGGCTTATTGGCTCAGGAACGCAAGCTTTGCCGAGATCTAGTCCATAGCAACAAAAAGGAACAGGAGTT
  TCGTTCCATTTTCCAGCACATACAATCAGCTCAGTCTCAGCGTAGCCCCTCAGAACTGTTTGCCCAACAT
  ATAGTGACCATTGTTCACCATGTTAAAGAGCATCACTTTGGGTCCTCAGGAATGACATTACATGAACGCT
  TTACTAAATACCTAAAGAGAGGAACTGAGCAGGAGGCAGCCAAAAACAAGAAAAGCCCAGAGATACACAG
  GAGAATAGACATTTCCCCCAGTACATTCAGAAAACATGGTTTGGCTCATGATGAAATGAAAAGTCCCCGG
  GAACCTGGCTACAAGGCTGAGGGAAAATACAAAGATGATCCTGTTGATCTCCGCCTTGATATTGAACGTC
  GTAAAAAACATAAGGAGAGAGATCTTAAACGAGGTAAATCGAGAGAATCAGTGGATTCCCGAGACTCCAG
  TCACTCAAGGGAAAGGTCAGCTGAAAAAACAGAGAAAACTCATAAAGGATCAAAGAAACAGAAGAAGCAT
  CGGAGAGCAAGAGACAGGTCCAGATCCTCCTCCTCTTCCTCCCAGTCATCTCACTCCTACAAAGCAGAAG
  AGTACACTGAAGAGACAGAGGAAAGAGAGGAGAGCACCACGGGCTTTGACAAATCAAGACTGGGGACCAA
  AGACTTTGTGGGTCCAAGTGAAAGAGGAGGTGGCAGAGCTCGAGGAACCTTTCAGTTTCGAGCCAGAGGA
  AGAGGCTGGGGCAGAGGCAACTACTCTGGGAACAATAACAACAACAGCAACAACGATTTTCAAAAAAGAA
  ACCGGGAAGAGGAGTGGGACCCAGAGTACACACCCAAAAGCAAGAAGTATTACTTGCATGATGACCGTGA
  AGGCGAAGGCAGTGACAAGTGGGTGAGCCGGGGCCGGGGCCGAGGAGCCTTTCCTCGGGGTCGGGGCCGG
  TTCATGTTCCGGAAATCAAGTACCAGCCCCAAGTGGGCCCATGACAAGTTCAGTGGGGAGGAAGGGGAGA
  TTGAAGACGACGAGAGTGGGACAGAGAACCGAGAAGAGAAGGACAATATACAGCCCACAACCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213342 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSKTNKSKSGSRSSRSRSASRSRSRSFSKSRSRSRSLSRSRKRRLSSRSRSRSYSPAHNRERNHPRVYQN
  RDFRGHNRGYRRPYYFRGRNRGFYPWGQYNRGGYGNYRSNWQNYRQAYSPRRGRSRSRSPKRRSPSPRSR
  SHSRNSDKSSSDRSRRSSSSRSSSNHSRVESSKRKSAKEKKSSSKDSRPSQAAGDNQGDEAKEQTFSGGT
  SQDTKASESSKPWPDATYGTGSASRASAVSELSPRERSPALKSPLQSVVVRRRSPRPSPVPKPSPPLSST
  SQMGSTLPSGAGYQSGTHQGQFDHGSGSLSPSKKSPVGKSPPSTGSTYGSSQKEESAASGGAAYTKRYLE
  EQKTENGKDKEQKQTNTDKEKIKEKGSFSDTGLGDGKMKSDSFAPKTDSEKPFRGSQSPKRYKLRDDFEK
  KMADFHKEEMDDQDKDKAKGRKESEFDDEPKFMSKVIGANKNQEEEKSGKWEGLVYAPPGKEKQRKTEEL
  EEESFPERSKKEDRGKRSEGGHRGFVPEKNFRVTAYKAVQEKSSSPPPRKTSESRDKLGAKGDFPTGKSS
  FSITREAQVNVRMDSFDEDLARPSGLLAQERKLCRDLVHSNKKEQEFRSIFQHIQSAQSQRSPSELFAQH
  IVTIVHHVKEHHFGSSGMTLHERFTKYLKRGTEQEAAKNKKSPEIHRRIDISPSTFRKHGLAHDEMKSPR
  EPGYKAEGKYKDDPVDLRLDIERRKKHKERDLKRGKSRESVDSRDSSHSRERSAEKTEKTHKGSKKQKKH
  RRARDRSRSSSSSSQSSHSYKAEEYTEETEEREESTTGFDKSRLGTKDFVGPSERGGGRARGTFQFRARG
  RGWGRGNYSGNNNNNSNNDFQKRNREEEWDPEYTPKSKKYYLHDDREGEGSDKWVSRGRGRGAFPRGRGR
  FMFRKSSTSPKWAHDKFSGEEGEIEDDESGTENREEKDNIQPTTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005119
• ORF Size: 2865 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005119.1, NM_005119.2, NM_005119.3, NP_005110.1, NP_005110.2
• RefSeq Size: 4447 bp
• RefSeq ORF: 2868 bp
• Locus ID: 9967
• Protein Families: Druggable Genome, Transcription Factors
• MW: 108.7 kDa