CD109 (NM_133493) Human Tagged ORF Clone

CAT#: RC213359

CD109 (Myc-DDK-tagged)-Human CD109 molecule (CD109), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CD109
• Synonyms: CPAMD7; p180; r150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213359 representing NM_133493
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGCCCACCGCTCCTGACCGCCGCCCACCTCCTCTGCGTGTGCACCGCCGCGCTGGCCGTGGCTC
  CCGGGCCTCGGTTTCTGGTGACAGCCCCAGGGATCATCAGGCCCGGAGGAAATGTGACTATTGGGGTGGA
  GCTTCTGGAACACTGCCCTTCACAGGTGACTGTGAAGGCGGAGCTGCTCAAGACAGCATCAAACCTCACT
  GTCTCTGTCCTGGAAGCAGAAGGAGTCTTTGAAAAAGGCTCTTTTAAGACACTTACTCTTCCATCACTAC
  CTCTGAACAGTGCAGATGAGATTTATGAGCTACGTGTAACCGGACGTACCCAGGATGAGATTTTATTCTC
  TAATAGTACCCGCTTATCATTTGAGACCAAGAGAATATCTGTCTTCATTCAAACAGACAAGGCCTTATAC
  AAGCCAAAGCAAGAAGTGAAGTTTCGCATTGTTACACTCTTCTCAGATTTTAAGCCTTACAAAACCTCTT
  TAAACATTCTCATTAAGGACCCCAAATCAAATTTGATCCAACAGTGGTTGTCACAACAAAGTGATCTTGG
  AGTCATTTCCAAAACTTTTCAGCTATCTTCCCATCCAATACTTGGTGACTGGTCTATTCAAGTTCAAGTG
  AATGACCAGACATATTATCAATCATTTCAGGTTTCAGAATATGTATTACCAAAATTTGAAGTGACTTTGC
  AGACACCATTATATTGTTCTATGAATTCTAAGCATTTAAATGGTACCATCACGGCAAAGTATACATATGG
  GAAGCCAGTGAAAGGAGACGTAACGCTTACATTTTTACCTTTATCCTTTTGGGGAAAGAAGAAAAATATT
  ACAAAAACATTTAAGATAAATGGATCTGCAAACTTCTCTTTTAATGATGAAGAGATGAAAAATGTAATGG
  ATTCTTCAAATGGACTTTCTGAATACCTGGATCTATCTTCCCCTGGACCAGTAGAAATTTTAACCACAGT
  GACAGAATCAGTTACAGGTATTTCAAGAAATGTAAGCACTAATGTGTTCTTCAAGCAACATGATTACATC
  ATTGAGTTTTTTGATTATACTACTGTCTTGAAGCCATCTCTCAACTTCACAGCCACTGTGAAGGTAACTC
  GTGCTGATGGCAACCAACTGACTCTTGAAGAAAGAAGAAATAATGTAGTCATAACAGTGACACAGAGAAA
  CTATACTGAGTACTGGAGCGGATCTAACAGTGGAAATCAGAAAATGGAAGCTGTTCAGAAAATAAATTAT
  ACTGTCCCCCAAAGTGGAACTTTTAAGATTGAATTCCCAATCCTGGAGGATTCCAGTGAGCTACAGTTGA
  AGGCCTATTTCCTTGGTAGTAAAAGTAGCATGGCAGTTCATAGTCTGTTTAAGTCTCCTAGTAAGACATA
  CATCCAACTAAAAACAAGAGATGAAAATATAAAGGTGGGATCGCCTTTTGAGTTGGTGGTTAGTGGCAAC
  AAACGATTGAAGGAGTTAAGCTATATGGTAGTATCCAGGGGACAGTTGGTGGCTGTAGGAAAACAAAATT
  CAACAATGTTCTCTTTAACACCAGAAAATTCTTGGACTCCAAAAGCCTGTGTAATTGTGTATTATATTGA
  AGATGATGGGGAAATTATAAGTGATGTTCTAAAAATTCCTGTTCAGCTTGTTTTTAAAAATAAGATAAAG
  CTATATTGGAGTAAAGTGAAAGCTGAACCATCTGAGAAAGTCTCTCTTAGGATCTCTGTGACACAGCCTG
  ACTCCATAGTTGGGATTGTAGCTGTTGACAAAAGTGTGAATCTGATGAATGCCTCTAATGATATTACAAT
  GGAAAATGTGGTCCATGAGTTGGAACTTTATAACACAGGATATTATTTAGGCATGTTCATGAATTCTTTT
  GCAGTCTTTCAGGAATGTGGACTCTGGGTATTGACAGATGCAAACCTCACGAAGGATTATATTGATGGTG
  TTTATGACAATGCAGAATATGCTGAGAGGTTTATGGAGGAAAATGAAGGACATATTGTAGATATTCATGA
  CTTTTCTTTGGGTAGCAGTCCACATGTCCGAAAGCATTTTCCAGAGACTTGGATTTGGCTAGACACCAAC
  ATGGGTTACAGGATTTACCAAGAATTTGAAGTAACTGTACCTGATTCTATCACTTCTTGGGTGGCTACTG
  GTTTTGTGATCTCTGAGGACCTGGGTCTTGGACTAACAACTACTCCAGTGGAGCTCCAAGCCTTCCAACC
  ATTTTTCATTTTTTTGAATCTTCCCTACTCTGTTATCAGAGGTGAAGAATTTGCTTTGGAAATAACTATA
  TTCAATTATTTGAAAGATGCCACTGAGGTTAAGGTAATCATTGAGAAAAGTGACAAATTTGATATTCTAA
  TGACTTCAAATGAAATAAATGCCACAGGCCACCAGCAGACCCTTCTGGTTCCCAGTGAGGATGGGGCAAC
  TGTTCTTTTTCCCATCAGGCCAACACATCTGGGAGAAATTCCTATCACAGTCACAGCTCTTTCACCCACT
  GCTTCTGATGCTGTCACCCAGATGATTTTAGTAAAGGCTGAAGGAATAGAAAAATCATATTCACAATCCA
  TCTTATTAGACTTGACTGACAATAGGCTACAGAGTACCCTGAAAACTTTGAGTTTCTCATTTCCTCCTAA
  TACAGTGACTGGCAGTGAAAGAGTTCAGATCACTGCAATTGGAGATGTTCTTGGTCCTTCCATCAATGGC
  TTAGCCTCATTGATTCGGATGCCTTATGGCTGTGGTGAACAGAACATGATAAATTTTGCTCCAAATATTT
  ACATTTTGGATTATCTGACTAAAAAGAAACAACTGACAGATAATTTGAAAGAAAAAGCTCTTTCATTTAT
  GAGGCAAGGTTACCAGAGAGAACTTCTCTATCAGAGGGAAGATGGCTCTTTCAGTGCTTTTGGGAATTAT
  GACCCTTCTGGGAGCACTTGGTTGTCAGCTTTTGTTTTAAGATGTTTCCTTGAAGCCGATCCTTACATAG
  ATATTGATCAGAATGTGTTACACAGAACATACACTTGGCTTAAAGGACATCAGAAATCCAACGGTGAATT
  TTGGGATCCAGGAAGAGTGATTCATAGTGAGCTTCAAGGTGGCAATAAAAGTCCAGTAACACTTACAGCC
  TATATTGTAACTTCTCTCCTGGGATATAGAAAGTATCAGCCTAACATTGATGTGCAAGAGTCTATCCATT
  TTTTGGAGTCTGAATTCAGTAGAGGAATTTCAGACAATTATACTCTAGCCCTTATAACTTATGCATTGTC
  ATCAGTGGGGAGTCCTAAAGCGAAGGAAGCTTTGAATATGCTGACTTGGAGAGCAGAACAAGAAGGTGGC
  ATGCAATTCTGGGTGTCATCAGAGTCCAAACTTTCTGACTCCTGGCAGCCACGCTCCCTGGATATTGAAG
  TTGCAGCCTATGCACTGCTCTCACACTTCTTACAATTTCAGACTTCTGAGGGAATCCCAATTATGAGGTG
  GCTAAGCAGGCAAAGAAATAGCTTGGGTGGTTTTGCATCTACTCAGGATACCACTGTGGCTTTAAAGGCT
  CTGTCTGAATTTGCAGCCCTAATGAATACAGAAAGGACAAATATCCAAGTGACCGTGACGGGGCCTAGCT
  CACCAAGTCCTGTAAAGTTTCTGATTGACACACACAACCGCTTACTCCTTCAGACAGCAGAGCTTGCTGT
  GGTACAGCCAATGGCAGTTAATATTTCCGCAAATGGTTTTGGATTTGCTATTTGTCAGCTCAATGTTGTA
  TATAATGTGAAGGCTTCTGGGTCTTCTAGAAGACGAAGATCTATCCAAAATCAAGAAGCCTTTGATTTAG
  ATGTTGCTGTAAAAGAAAATAAAGATGATCTCAATCATGTGGATTTGAATGTGTGTACAAGCTTTTCGGG
  CCCGGGTAGGAGTGGCATGGCTCTTATGGAAGTTAACCTATTAAGTGGCTTTATGGTGCCTTCAGAAGCA
  ATTTCTCTGAGCGAGACAGTGAAGAAAGTGGAATATGATCATGGAAAACTCAACCTCTATTTAGATTCTG
  TAAATGAAACCCAGTTTTGTGTTAATATTCCTGCTGTGAGAAACTTTAAAGTTTCAAATACCCAAGATGC
  TTCAGTGTCCATAGTGGATTACTATGAGCCAAGGAGACAGGCGGTGAGAAGTTACAACTCTGAAGTGAAG
  CTGTCCTCCTGTGACCTTTGCAGTGATGTCCAGGGCTGCCGTCCTTGTGAGGATGGAGCTTCAGGCTCCC
  ATCATCACTCTTCAGTCATTTTTATTTTCTGTTTCAAGCTTCTGTACTTTATGGAACTTTGGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213359 representing NM_133493
  Red=Cloning site Green=Tags(s)
  
  MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLT
  VSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALY
  KPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQV
  NDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNI
  TKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYI
  IEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINY
  TVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGN
  KRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIK
  LYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSF
  AVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTN
  MGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITI
  FNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPT
  ASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSING
  LASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNY
  DPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTA
  YIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGG
  MQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKA
  LSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPMAVNISANGFGFAICQLNVV
  YNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEA
  ISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVK
  LSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133493
• ORF Size: 4335 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133493.1, NM_133493.2, NM_133493.3, NM_133493.4, NP_598000.1
• RefSeq Size: 5883 bp
• RefSeq ORF: 4338 bp
• Locus ID: 135228
• Domains: A2M, A2M_N
• MW: 159.6 kDa
• Gene Summary: This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]