TBL1 (TBL1X) (NM_005647) Human Tagged ORF Clone

CAT#: RC213516

TBL1X (Myc-DDK-tagged)-Human transducin (beta)-like 1X-linked (TBL1X), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427964) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226915 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TBL1X protein (Cat# TP313516). The protein was produced from HEK293T cells transfected with TBL1X cDNA clone (Cat# RC213516) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBL1X
• Synonyms: EBI; SMAP55; TBL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213516 representing NM_005647
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGAGCTCGCTGGCGCCTCTTCATCGTGCTGCCACCGCCCTGCAGGAAGAGGGGCCATGCAGTCAG
  TCTTGCACCACTTTCAACGTTTGCGAGGGAGAGAGGGTGGTTCCCACTTCATCAACACCTCATCGCCGCG
  AGGTGAGGCTAAGATGAGCATAACCAGTGACGAGGTGAACTTTCTGGTGTATCGGTATCTCCAGGAGTCA
  GGTTTTTCCCACTCGGCTTTCACGTTTGGGATTGAGAGCCACATCAGCCAGTCCAACATCAATGGGACGC
  TAGTGCCACCGGCCGCCCTCATCTCCATTCTCCAGAAGGGCCTGCAGTATGTAGAGGCCGAGATCAGTAT
  CAACGAGGATGGCACAGTGTTCGACGGCCGCCCCATAGAGTCCCTGTCACTGATAGACGCCGTGATGCCC
  GACGTGGTGCAGACGCGGCAGCAGGCATTCCGAGAGAAGCTCGCTCAGCAGCAAGCCAGTGCGGCGGCGG
  CGGCGGCTGCGGCCACGGCAGCAGCGACAGCAGCCACCACGACCTCAGCCGGCGTTTCCCACCAAAATCC
  ATCGAAGAACAGAGAGGCCACGGTGAATGGGGAAGAGAACAGAGCACATTCAGTCAATAATCACGCGAAG
  CCAATGGAAATAGATGGAGAGGTTGAGATTCCATCCAGCAAAGCCACAGTCCTTCGGGGCCATGAGTCTG
  AGGTGTTCATTTGTGCCTGGAATCCTGTCAGTGATTTGCTAGCCTCCGGATCTGGAGACTCAACTGCAAG
  GATATGGAACCTGAATGAGAATAGCAACGGGGGCTCCACCCAGCTCGTGTTGAGGCACTGTATACGAGAG
  GGGGGCCATGACGTCCCGAGTAACAAAGACGTCACCTCACTGGACTGGAATACCAATGGAACACTCTTGG
  CTACGGGTTCATATGACGGTTTTGCAAGAATATGGACGGAAGATGGTAACCTGGCCAGCACCTTAGGCCA
  ACATAAAGGCCCCATCTTTGCCTTGAAATGGAACCGAAAGGGGAATTACATTTTGAGTGCTGGTGTAGAC
  AAAACAACAATAATTTGGGATGCCCACACAGGAGAAGCCAAACAGCAGTTTCCTTTTCATTCAGCCCCTG
  CCCTTGATGTGGACTGGCAGAACAACACGACCTTTGCCTCCTGTAGCACAGACATGTGTATCCATGTGTG
  CAGGCTCGGCTGTGACCGCCCAGTCAAAACCTTCCAGGGACACACAAACGAGGTCAACGCCATCAAATGG
  GATCCGTCTGGAATGTTGCTGGCATCCTGCTCGGATGACATGACATTGAAGATCTGGAGCATGAAACAGG
  AGGTGTGCATCCATGATCTTCAGGCTCACAATAAAGAGATCTACACCATCAAGTGGAGCCCCACTGGGCC
  CGCCACCAGCAACCCAAACTCCAACATCATGTTGGCAAGTGCTTCGTTTGATTCTACGGTGCGACTGTGG
  GACATAGAACGAGGCGTCTGCACCCACACGCTCACGAAGCATCAGGAGCCTGTCTATAGCGTAGCTTTCA
  GCCCTGATGGGAAGTACTTGGCCAGTGGATCCTTCGACAAGTGCGTCCATATCTGGAATACTCAGAGTGG
  AAATCTTGTCCACAGCTACCGAGGCACTGGCGGCATCTTCGAGGTGTGCTGGAACGCCCGAGGAGACAAA
  GTGGGTGCCAGCGCGTCCGACGGCTCTGTGTGTGTTTTGGATCTGCGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213516 representing NM_005647
  Red=Cloning site Green=Tags(s)
  
  MTELAGASSSCCHRPAGRGAMQSVLHHFQRLRGREGGSHFINTSSPRGEAKMSITSDEVNFLVYRYLQES
  GFSHSAFTFGIESHISQSNINGTLVPPAALISILQKGLQYVEAEISINEDGTVFDGRPIESLSLIDAVMP
  DVVQTRQQAFREKLAQQQASAAAAAAAATAAATAATTTSAGVSHQNPSKNREATVNGEENRAHSVNNHAK
  PMEIDGEVEIPSSKATVLRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNENSNGGSTQLVLRHCIRE
  GGHDVPSNKDVTSLDWNTNGTLLATGSYDGFARIWTEDGNLASTLGQHKGPIFALKWNRKGNYILSAGVD
  KTTIIWDAHTGEAKQQFPFHSAPALDVDWQNNTTFASCSTDMCIHVCRLGCDRPVKTFQGHTNEVNAIKW
  DPSGMLLASCSDDMTLKIWSMKQEVCIHDLQAHNKEIYTIKWSPTGPATSNPNSNIMLASASFDSTVRLW
  DIERGVCTHTLTKHQEPVYSVAFSPDGKYLASGSFDKCVHIWNTQSGNLVHSYRGTGGIFEVCWNARGDK
  VGASASDGSVCVLDLRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005647
• ORF Size: 1731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005647.1, NM_005647.2, NM_005647.3, NP_005638.1
• RefSeq Size: 5886 bp
• RefSeq ORF: 1734 bp
• Locus ID: 6907
• Cytogenetics: Xp22.31-p22.2
• Domains: WD40, LisH
• Protein Families: Transcription Factors
• Protein Pathways: Wnt signaling pathway
• MW: 62.3 kDa
• Gene Summary: 'The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation. This encoded protein is found as a subunit in corepressor SMRT (silencing mediator for retinoid and thyroid receptors) complex along with histone deacetylase 3 protein. This gene is located adjacent to the ocular albinism gene and it is thought to be involved in the pathogenesis of the ocular albinism with late-onset sensorineural deafness phenotype. Four transcript variants encoding two different isoforms have been found for this gene. This gene is highly similar to the Y chromosome TBL1Y gene. [provided by RefSeq, Nov 2008]'