NHLRC1 (NM_198586) Human Tagged ORF Clone

CAT#: RC213526

NHLRC1 (Myc-DDK-tagged)-Human NHL repeat containing 1 (NHLRC1)

Product Images

Western blot validation of overexpression lysate (Cat# LY404837) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213526 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NHLRC1
• Synonyms: bA204B7.2; EPM2A; EPM2B; MALIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213526 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCGGCCGAAGCCTCGGAGAGCGGGCCAGCGCTGCATGAGCTCATGCGCGAGGCGGAGATCAGCCTGC
  TCGAGTGCAAGGTGTGCTTTGAGAAGTTTGGCCACCGGCAGCAGCGGCGCCCGCGCAACCTGTCCTGCGG
  CCACGTGGTCTGCCTGGCCTGCGTGGCCGCCCTGGCGCACCCGCGCACTCTGGCCCTCGAGTGCCCATTC
  TGCAGGCGAGCTTGCCGGGGCTGCGACACCAGCGACTGCCTGCCGGTGCTGCACCTCATAGAGCTCCTGG
  GCTCAGCGCTTCGCCAGTCCCCGGCCGCCCATCGCGCCGCCCCCAGCGCCCCCGGAGCCCTCACCTGCCA
  CCACACCTTCGGCGGCTGGGGGACCCTGGTCAACCCCACCGGACTGGCGCTTTGTCCCAAGACGGGGCGT
  GTCGTGGTGGTGCACGACGGCAGGAGGCGTGTCAAGATTTTTGACTCAGGGGGAGGATGCGCGCATCAGT
  TTGGAGAGAAGGGGGACGCTGCCCAAGACATTAGGTACCCTGTGGATGTCACCATCACCAACGACTGCCA
  TGTGGTTGTCACTGACGCCGGCGATCGCTCCATCAAAGTGTTTGATTTTTTTGGCCAGATCAAGCTTGTC
  ATTGGAGGCCAATTCTCCTTACCTTGGGGTGTGGAGACCACCCCTCAGAATGGGATTGTGGTAACTGATG
  CGGAGGCAGGGTCCCTGCACCTCCTGGACGTCGACTTCGCGGAAGGGGTCCTTCGGAGAACTGAAAGGTT
  GCAAGCTCATCTGTGCAATCCCCGAGGGGTGGCAGTGTCTTGGCTCACCGGGGCCATTGCGGTCCTGGAG
  CACCCCCTGGCCCTGGGGACTGGGGTTTGCAGCACCAGGGTGAAAGTGTTTAGCTCAAGTATGCAGCTTG
  TCGGCCAAGTGGATACCTTTGGGCTGAGCCTCTACTTTCCCTCCAAAATAACTGCCTCCGCTGTGACCTT
  TGATCACCAGGGAAATGTGATTGTTGCAGATACATCTGGTCCAGCTATCCTTTGCTTAGGAAAACCTGAG
  GAGTTTCCAGTACCGAAGCCCATGGTCACTCATGGTCTTTCGCATCCTGTGGCTCTTACCTTCACCAAGG
  AGAATTCTCTTCTTGTGCTGGACACAGCATCTCATTCTATAAAAGTCTATAAAGTTGACTGGGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213526 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAEASESGPALHELMREAEISLLECKVCFEKFGHRQQRRPRNLSCGHVVCLACVAALAHPRTLALECPF
  CRRACRGCDTSDCLPVLHLIELLGSALRQSPAAHRAAPSAPGALTCHHTFGGWGTLVNPTGLALCPKTGR
  VVVVHDGRRRVKIFDSGGGCAHQFGEKGDAAQDIRYPVDVTITNDCHVVVTDAGDRSIKVFDFFGQIKLV
  IGGQFSLPWGVETTPQNGIVVTDAEAGSLHLLDVDFAEGVLRRTERLQAHLCNPRGVAVSWLTGAIAVLE
  HPLALGTGVCSTRVKVFSSSMQLVGQVDTFGLSLYFPSKITASAVTFDHQGNVIVADTSGPAILCLGKPE
  EFPVPKPMVTHGLSHPVALTFTKENSLLVLDTASHSIKVYKVDWG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198586
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_198586.1, NM_198586.2, NP_940988.2
• RefSeq Size: 2134
• RefSeq ORF: 1188
• Locus ID: 378884
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 42.3 kDa
• Gene Summary: The protein encoded by this gene is a single subunit E3 ubiquitin ligase. Laforin is polyubiquitinated by the encoded protein. Defects in this intronless gene lead to an accumulation of laforin and onset of Lafora disease, also known as progressive myoclonic epilepsy type 2 (EPM2). [provided by RefSeq, Mar 2010]