Ribonuclease Inhibitor (RNH1) (NM_203387) Human Tagged ORF Clone

CAT#: RC213642

RNH1 (Myc-DDK-tagged)-Human ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 6

Product Images

Western blot validation of overexpression lysate (Cat# LY404327) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218365 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RNH1 protein (Cat# TP313642). The protein was produced from HEK293T cells transfected with RNH1 cDNA clone (Cat# RC213642) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNH1
• Synonyms: RAI; RNH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213642 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTGGACATCCAGAGCCTGGACATCCAGTGTGAGGAGCTGAGCGACGCTAGATGGGCCGAGCTCC
  TCCCTCTGCTCCAGCAGTGCCAAGTGGTCAGGCTGGACGACTGTGGCCTCACGGAAGCACGGTGCAAGGA
  CATCAGCTCTGCACTTCGAGTCAACCCTGCACTGGCAGAGCTCAACCTGCGCAGCAACGAGCTGGGCGAT
  GTCGGCGTGCATTGCGTGCTCCAGGGCCTGCAGACCCCCTCCTGCAAGATCCAGAAGCTGAGCCTCCAGA
  ACTGCTGCCTGACGGGGGCCGGCTGCGGGGTCCTGTCCAGCACACTACGCACCCTGCCCACCCTGCAGGA
  GCTGCACCTCAGCGACAACCTCTTGGGGGATGCGGGCCTGCAGCTGCTCTGCGAAGGACTCCTGGACCCC
  CAGTGCCGCCTGGAAAAGCTGCAGCTGGAGTATTGCAGCCTCTCGGCTGCCAGCTGCGAGCCCCTGGCCT
  CCGTGCTCAGGGCCAAGCCGGACTTCAAGGAGCTCACGGTTAGCAACAACGACATCAATGAGGCTGGCGT
  CCGTGTGCTGTGCCAGGGCCTGAAGGACTCCCCCTGCCAGCTGGAGGCGCTCAAGCTGGAGAGCTGCGGT
  GTGACATCAGACAACTGCCGGGACCTGTGCGGCATTGTGGCCTCCAAGGCCTCGCTGCGGGAGCTGGCCC
  TGGGCAGCAACAAGCTGGGTGATGTGGGCATGGCGGAGCTGTGCCCAGGGCTGCTCCACCCCAGCTCCAG
  GCTCAGGACCCTGTGGATCTGGGAGTGTGGCATCACTGCCAAGGGCTGCGGGGATCTGTGCCGTGTCCTC
  AGGGCCAAGGAGAGCCTGAAGGAGCTCAGCCTGGCCGGCAACGAGCTGGGGGATGAGGGTGCCCGACTGC
  TGTGTGAGACCCTGCTGGAACCTGGCTGCCAGCTGGAGTCGCTGTGGGTGAAGTCCTGCAGCTTCACAGC
  CGCCTGCTGCTCCCACTTCAGCTCAGTGCTGGCCCAGAACAGGTTTCTCCTGGAGCTACAGATAAGCAAC
  AACAGGCTGGAGGATGCGGGCGTGCGGGAGCTGTGCCAGGGCCTGGGCCAGCCTGGCTCTGTGCTGCGGG
  TGCTCTGGTTGGCCGACTGCGATGTGAGTGACAGCAGCTGCAGCAGCCTCGCCGCAACCCTGTTGGCCAA
  CCACAGCCTGCGTGAGCTGGACCTCAGCAACAACTGCCTGGGGGACGCCGGCATCCTGCAGCTGGTGGAG
  AGCGTCCGGCAGCCGGGCTGCCTCCTGGAGCAGCTGGTCCTGTACGACATTTACTGGTCTGAGGAGATGG
  AGGACCGGCTGCAGGCCCTGGAGAAGGACAAGCCATCCCTGAGGGTCATCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213642 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDDCGLTEARCKDISSALRVNPALAELNLRSNELGD
  VGVHCVLQGLQTPSCKIQKLSLQNCCLTGAGCGVLSSTLRTLPTLQELHLSDNLLGDAGLQLLCEGLLDP
  QCRLEKLQLEYCSLSAASCEPLASVLRAKPDFKELTVSNNDINEAGVRVLCQGLKDSPCQLEALKLESCG
  VTSDNCRDLCGIVASKASLRELALGSNKLGDVGMAELCPGLLHPSSRLRTLWIWECGITAKGCGDLCRVL
  RAKESLKELSLAGNELGDEGARLLCETLLEPGCQLESLWVKSCSFTAACCSHFSSVLAQNRFLLELQISN
  NRLEDAGVRELCQGLGQPGSVLRVLWLADCDVSDSSCSSLAATLLANHSLRELDLSNNCLGDAGILQLVE
  SVRQPGCLLEQLVLYDIYWSEEMEDRLQALEKDKPSLRVIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203387
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203387.1, NM_203387.2, NP_976321.1
• RefSeq Size: 2015 bp
• RefSeq ORF: 1386 bp
• Locus ID: 6050
• Cytogenetics: 11p15.5
• MW: 50 kDa
• Gene Summary: 'Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM, Jul 2010]'