CCDC90B (NM_021825) Human Tagged ORF Clone

CAT#: RC213680

CCDC90B (Myc-DDK-tagged)-Human coiled-coil domain containing 90B (CCDC90B)

Product Images

Western blot validation of overexpression lysate (Cat# LY411907) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213680 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CCDC90B protein (Cat# TP313680). The protein was produced from HEK293T cells transfected with CCDC90B cDNA clone (Cat# RC213680) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC90B
• Synonyms: MDS011; MDS025
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213680 representing NM_021825
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAGTCGCCAGGCTTGGCGGCTCTTTCTCTCCCAAGGCAGAGGAGATCGTTGGGTTTCAAGGCCCC
  GCGGGCATTTCTCGCCGGCCCTGCGGAGAGAGTTCTTCACTACCACAACCAAGGAGGGATATGATAGGCG
  GCCAGTGGATATAACTCCTTTAGAACAAAGGAAATTAACTTTTGATACCCATGCATTGGTTCAGGACTTG
  GAAACTCATGGATTTGACAAAACACAAGCAGAAACAATTGTATCAGCGTTAACTGCTTTATCAAATGTCA
  GCCTGGATACTATCTATAAAGAGATGGTCACTCAAGCTCAACAGGAAATAACAGTACAACAGCTAATGGC
  TCATTTGGATGCTATCAGGAAAGACATGGTCATCCTAGAGAAAAGTGAATTTGCAAATCTGAGAGCAGAG
  AATGAGAAAATGAAAATTGAATTAGACCAAGTTAAGCAACAACTAATGCATGAAACCAGTCGAATCAGAG
  CAGATAATAAACTGGATATCAACTTAGAAAGGAGCAGAGTAACAGATATGTTTACAGATCAAGAAAAGCA
  ACTTATGGAAACAACTACAGAATTTACAAAAAAGGATACTCAAACCAAAAGTATTATTTCAGAGACCAGT
  AATAAAATTGACGCTGAAATTGCTTCCTTAAAAACACTGATGGAATCTAACAAACTTGAGACAATTCGTT
  ATCTTGCAGCTTCAGTGTTTACTTGCCTGGCAATAGCATTGGGATTTTATAGATTCTGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213680 representing NM_021825
  Red=Cloning site Green=Tags(s)
  
  MNSRQAWRLFLSQGRGDRWVSRPRGHFSPALRREFFTTTTKEGYDRRPVDITPLEQRKLTFDTHALVQDL
  ETHGFDKTQAETIVSALTALSNVSLDTIYKEMVTQAQQEITVQQLMAHLDAIRKDMVILEKSEFANLRAE
  NEKMKIELDQVKQQLMHETSRIRADNKLDINLERSRVTDMFTDQEKQLMETTTEFTKKDTQTKSIISETS
  NKIDAEIASLKTLMESNKLETIRYLAASVFTCLAIALGFYRFWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021825
• ORF Size: 762 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021825.1, NM_021825.2, NM_021825.3, NM_021825.4, NP_068597.2
• RefSeq Size: 1585
• RefSeq ORF: 765
• Locus ID: 60492
• Protein Families: Transmembrane
• MW: 29.3 kDa