Eph receptor A1 (EPHA1) (NM_005232) Human Tagged ORF Clone

CAT#: RC213689

EPHA1 (Myc-DDK-tagged)-Human EPH receptor A1 (EPHA1)

Product Images

Western blot validation of overexpression lysate (Cat# LY401608) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213689 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EPHA1 protein (Cat# TP313689). The protein was produced from HEK293T cells transfected with EPHA1 cDNA clone (Cat# RC213689) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EPHA1
• Synonyms: EPH; EPHT; EPHT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213689 representing NM_005232
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCGCTGGCCCCTGGGGCTAGGGCTGGTGCTGCTGCTCTGCGCCCCGCTGCCCCCGGGGGCTC
  ACGCCAAGGAAGTTACTCTGATGGACACAAGCAAGGCACAGGGAGAGCTGGGCTGGCTGCTGGATCCCCC
  AAAAGATGGGTGGAGTGAACAGCAACAGATACTGAATGGGACACCCCTGTACATGTACCAGGACTGCCCA
  ATGCAAGGACGCAGAGACACTGACCACTGGCTTCGCTCCAATTGGATCTACCGCGGGGAGGAGGCTTCCC
  GCGTCCACGTGGAGCTGCAGTTCACCGTGCGGGACTGCAAGAGTTTCCCTGGGGGAGCCGGGCCTCTGGG
  CTGCAAGGAGACCTTCAACCTTCTGTACATGGAGAGTGACCAGGATGTGGGCATTCAGCTCCGACGGCCC
  TTGTTCCAGAAGGTAACCACGGTGGCTGCAGACCAGAGCTTCACCATTCGAGACCTTGCGTCTGGCTCCG
  TGAAGCTGAATGTGGAGCGCTGCTCTCTGGGCCGCCTGACCCGCCGTGGCCTCTACCTCGCTTTCCACAA
  CCCGGGTGCCTGTGTGGCCCTGGTGTCTGTCCGGGTCTTCTACCAGCGCTGTCCTGAGACCCTGAATGGC
  TTGGCCCAATTCCCAGACACTCTGCCTGGCCCCGCTGGGTTGGTGGAAGTGGCGGGGACCTGCTTGCCCC
  ACGCGCGGGCCAGCCCCAGGCCCTCAGGTGCACCCCGCATGCACTGCAGCCCTGATGGCGAGTGGCTGGT
  GCCTGTAGGACGGTGCCACTGTGAGCCTGGCTATGAGGAAGGTGGCAGTGGCGAAGCATGTGTTGCCTGC
  CCTAGCGGCTCCTACCGGATGGACATGGACACACCCCATTGTCTCACGTGCCCCCAGCAGAGCACTGCTG
  AGTCTGAGGGGGCCACCATCTGTACCTGTGAGAGCGGCCATTACAGAGCTCCCGGGGAGGGCCCCCAGGT
  GGCATGCACAGGTCCCCCCTCGGCCCCCCGAAACCTGAGCTTCTCTGCCTCAGGGACTCAGCTCTCCCTG
  CGTTGGGAACCCCCAGCAGATACGGGGGGACGCCAGGATGTCAGATACAGTGTGAGGTGTTCCCAGTGTC
  AGGGCACAGCACAGGACGGGGGGCCCTGCCAGCCCTGTGGGGTGGGCGTGCACTTCTCGCCGGGGGCCCG
  GGGGCTCACCACACCTGCAGTGCATGTCAATGGCCTTGAACCTTATGCCAACTACACCTTTAATGTGGAA
  GCCCAAAATGGAGTGTCAGGGCTGGGCAGCTCTGGCCATGCCAGCACCTCAGTCAGCATCAGCATGGGGC
  ATGCAGAGTCACTGTCAGGCCTGTCTCTGAGACTGGTGAAGAAAGAACCGAGGCAACTAGAGCTGACCTG
  GGCGGGGTCCCGGCCCCGAAGCCCTGGGGCGAACCTGACCTATGAGCTGCACGTGCTGAACCAGGATGAA
  GAACGGTACCAGATGGTTCTAGAACCCAGGGTCTTGCTGACAGAGCTGCAGCCTGACACCACATACATCG
  TCAGAGTCCGAATGCTGACCCCACTGGGTCCTGGCCCTTTCTCCCCTGATCATGAGTTTCGGACCAGCCC
  ACCAGTGTCCAGGGGCCTGACTGGAGGAGAGATTGTAGCCGTCATCTTTGGGCTGCTGCTTGGTGCAGCC
  TTGCTGCTTGGGATTCTCGTTTTCCGGTCCAGGAGAGCCCAGCGGCAGAGGCAGCAGAGGCAGCGTGACC
  GCGCCACCGATGTGGATCGAGAGGACAAGCTGTGGCTGAAGCCTTATGTGGACCTCCAGGCATACGAGGA
  CCCTGCACAGGGAGCCTTGGACTTTACCCGGGAGCTTGATCCAGCGTGGCTGATGGTGGACACTGTCATA
  GGAGAAGGAGAGTTTGGGGAAGTGTATCGAGGGACCCTGAGGCTCCCCAGCCAGGACTGCAAGACTGTGG
  CCATTAAGACCTTAAAAGACACATCCCCAGGTGGCCAGTGGTGGAACTTCCTTCGAGAGGCAACTATCAT
  GGGCCAGTTTAGCCACCCGCATATTCTGCATCTGGAAGGCGTCGTCACAAAGCGAAAGCCGATCATGATC
  ATCACAGAATTTATGGAGAATGGAGCCCTGGATGCCTTCCTGAGGGAGCGGGAGGACCAGCTGGTCCCTG
  GGCAGCTAGTGGCCATGCTGCAGGGCATAGCATCTGGCATGAACTACCTCAGTAATCACAATTATGTCCA
  CCGGGACCTGGCTGCCAGAAACATCTTGGTGAATCAAAACCTGTGCTGCAAGGTGTCTGACTTTGGCCTG
  ACTCGCCTCCTGGATGACTTTGATGGCACATACGAAACCCAGGGAGGAAAGATCCCTATCCGTTGGACAG
  CCCCTGAAGCCATTGCCCATCGGATCTTCACCACAGCCAGCGATGTGTGGAGCTTTGGGATTGTGATGTG
  GGAGGTGCTGAGCTTTGGGGACAAGCCTTATGGGGAGATGAGCAATCAGGAGGTTATGAAGAGCATTGAG
  GATGGGTACCGGTTGCCCCCTCCTGTGGACTGCCCTGCCCCTCTGTATGAGCTCATGAAGAACTGCTGGG
  CATATGACCGTGCCCGCCGGCCACACTTCCAGAAGCTTCAGGCACATCTGGAGCAACTGCTTGCCAACCC
  CCACTCCCTGCGGACCATTGCCAACTTTGACCCCAGGGTGACTCTTCGCCTGCCCAGCCTGAGTGGCTCA
  GATGGGATCCCGTATCGAACCGTCTCTGAGTGGCTCGAGTCCATACGCATGAAACGCTACATCCTGCACT
  TCCACTCGGCTGGGCTGGACACCATGGAGTGTGTGCTGGAGCTGACCGCTGAGGACCTGACGCAGATGGG
  AATCACACTGCCCGGGCACCAGAAGCGCATTCTTTGCAGTATTCAGGGATTCAAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213689 representing NM_005232
  Red=Cloning site Green=Tags(s)
  
  MERRWPLGLGLVLLLCAPLPPGAHAKEVTLMDTSKAQGELGWLLDPPKDGWSEQQQILNGTPLYMYQDCP
  MQGRRDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRP
  LFQKVTTVAADQSFTIRDLASGSVKLNVERCSLGRLTRRGLYLAFHNPGACVALVSVRVFYQRCPETLNG
  LAQFPDTLPGPAGLVEVAGTCLPHARASPRPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEACVAC
  PSGSYRMDMDTPHCLTCPQQSTAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASGTQLSL
  RWEPPADTGGRQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARGLTTPAVHVNGLEPYANYTFNVE
  AQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLTYELHVLNQDE
  ERYQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGEIVAVIFGLLLGAA
  LLLGILVFRSRRAQRQRQQRQRDRATDVDREDKLWLKPYVDLQAYEDPAQGALDFTRELDPAWLMVDTVI
  GEGEFGEVYRGTLRLPSQDCKTVAIKTLKDTSPGGQWWNFLREATIMGQFSHPHILHLEGVVTKRKPIMI
  ITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGL
  TRLLDDFDGTYETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIE
  DGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFQKLQAHLEQLLANPHSLRTIANFDPRVTLRLPSLSGS
  DGIPYRTVSEWLESIRMKRYILHFHSAGLDTMECVLELTAEDLTQMGITLPGHQKRILCSIQGFKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005232
• ORF Size: 2928 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005232.2, NM_005232.3, NM_005232.4, NP_005223.3, NP_005223.4
• RefSeq Size: 3367 bp
• RefSeq ORF: 2931 bp
• Locus ID: 2041
• Cytogenetics: 7q34-q35
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• MW: 107.9 kDa
• Gene Summary: 'This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]'