PICALM (NM_007166) Human Tagged ORF Clone
CAT#: RC213791L1
- LentiORF®
Lenti ORF clone of Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1, Myc-DDK-tagged
"NM_007166" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PICALM |
Synonyms | CALM; CLTH; LAP |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC213791).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_007166 |
ORF Size | 1956 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007166.2 |
RefSeq Size | 3860 bp |
RefSeq ORF | 1959 bp |
Locus ID | 8301 |
UniProt ID | Q13492 |
Cytogenetics | 11q14.2 |
Domains | ENTH |
Protein Families | Druggable Genome |
MW | 70.6 kDa |
Gene Summary | This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC127391 | PICALM (untagged)-Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1 |
USD 310.00 |
|
RC213791 | PICALM (Myc-DDK-tagged)-Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1 |
USD 420.00 |
|
RG213791 | PICALM (GFP-tagged) - Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1 |
USD 460.00 |
|
RC213791L2 | Lenti ORF clone of Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1, mGFP tagged |
USD 768.00 |
|
RC213791L3 | Lenti ORF clone of Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC213791L4 | Lenti ORF clone of Human phosphatidylinositol binding clathrin assembly protein (PICALM), transcript variant 1, mGFP tagged |
USD 620.00 |
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