Collagen IV (COL4A1) (NM_001845) Human Tagged ORF Clone
CAT#: RC213796
- TrueORF®
COL4A1 (Myc-DDK-tagged)-Human collagen, type IV, alpha 1 (COL4A1). Note: ORF is codon optimized
"NM_001845" in other vectors (2)
Product Images
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Other products for "COL4A1"
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | COL4A1 |
Synonyms | BSVD; BSVD1; RATOR |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213796 ORF sequence, codon optimized.
Due to the complexity of NM_001845, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACCGAGACTGTCTGTATGGTTGCTCCTGCTGCCCGCTGCCCTGCTGCTTCACGAGGAGCATTCCC GGGCTGCCGCGAAAGGGGGGTGTGCAGGCAGCGGCTGTGGTAAATGTGATTGTCACGGCGTCAAGGGCCA GAAGGGAGAGCGGGGTCTGCCGGGTCTGCAAGGTGTGATCGGATTTCCCGGAATGCAGGGTCCAGAGGGC CCACAGGGCCCACCCGGCCAGAAAGGCGACACCGGAGAGCCAGGCCTGCCTGGCACCAAAGGAACACGGG GACCACCTGGGGCTAGCGGATACCCTGGAAACCCCGGGCTGCCGGGGATTCCCGGCCAGGACGGGCCCCC CGGTCCACCCGGAATTCCAGGGTGTAACGGTACCAAGGGAGAACGCGGCCCACTGGGTCCCCCGGGCTTG CCTGGGTTCGCAGGCAACCCGGGACCCCCTGGGCTGCCGGGGATGAAGGGAGATCCCGGCGAAATCCTTG GTCATGTTCCCGGGATGCTGCTCAAGGGCGAAAGGGGATTCCCAGGCATCCCGGGGACGCCCGGTCCGCC TGGACTGCCAGGTCTGCAGGGACCTGTGGGCCCACCCGGGTTTACTGGGCCCCCAGGGCCACCTGGACCA CCCGGACCCCCAGGGGAGAAGGGACAGATGGGGCTGTCTTTTCAGGGTCCGAAAGGGGACAAAGGGGACC AAGGCGTGAGTGGCCCTCCTGGAGTGCCCGGCCAGGCCCAGGTACAGGAGAAGGGCGATTTTGCTACTAA AGGCGAAAAGGGACAAAAGGGTGAACCTGGCTTTCAAGGGATGCCGGGGGTTGGAGAGAAGGGGGAGCCA GGAAAACCAGGCCCGAGAGGCAAGCCTGGGAAGGATGGAGACAAAGGAGAGAAAGGAAGCCCCGGCTTTC CCGGTGAACCAGGGTACCCAGGACTGATTGGGCGCCAGGGACCACAGGGTGAGAAAGGAGAAGCAGGCCC TCCTGGACCTCCAGGAATCGTCATTGGAACAGGACCATTGGGGGAGAAGGGCGAGAGAGGATACCCCGGC ACCCCAGGGCCGCGGGGCGAACCAGGGCCTAAGGGGTTTCCAGGGCTGCCAGGTCAGCCAGGTCCTCCAG GTCTGCCGGTGCCAGGCCAGGCCGGAGCTCCTGGCTTCCCTGGTGAGAGAGGCGAAAAAGGCGACAGAGG TTTTCCAGGCACTTCTTTGCCTGGCCCGTCCGGCAGGGATGGCCTGCCGGGACCACCGGGCAGTCCCGGG CCCCCCGGTCAGCCTGGATATACAAATGGCATCGTGGAGTGTCAACCAGGACCCCCCGGAGATCAAGGCC CACCCGGAATCCCAGGGCAGCCTGGATTCATTGGCGAGATTGGAGAGAAGGGGCAGAAAGGGGAATCATG CCTGATATGTGATATTGATGGTTACAGAGGGCCACCCGGGCCCCAGGGCCCCCCCGGGGAAATCGGCTTT CCAGGTCAGCCAGGTGCAAAGGGGGACAGGGGACTGCCAGGAAGGGACGGGGTAGCCGGCGTGCCAGGCC CCCAGGGCACCCCTGGACTCATTGGTCAGCCCGGTGCCAAGGGTGAACCTGGGGAATTCTATTTTGATCT GCGCCTCAAAGGCGACAAAGGGGACCCGGGCTTCCCAGGCCAGCCCGGAATGCCTGGCAGAGCCGGGAGC CCAGGGCGAGATGGACACCCAGGTCTGCCAGGACCCAAGGGCAGCCCAGGAAGCGTTGGCCTGAAAGGGG AACGCGGTCCTCCCGGGGGCGTGGGATTCCCGGGAAGCCGGGGTGATACAGGACCTCCTGGCCCTCCTGG ATATGGTCCAGCTGGCCCTATCGGGGATAAGGGGCAGGCAGGGTTCCCTGGAGGGCCTGGGAGCCCCGGG CTTCCTGGACCAAAAGGCGAGCCTGGAAAAATTGTCCCTCTTCCCGGGCCACCTGGAGCGGAGGGTCTGC CAGGCTCACCCGGCTTTCCGGGACCCCAGGGGGACCGGGGATTCCCTGGTACACCCGGCCGCCCCGGCCT GCCCGGAGAAAAAGGGGCAGTAGGACAGCCTGGGATCGGATTCCCGGGACCGCCTGGACCCAAGGGAGTC GATGGGCTGCCGGGCGATATGGGGCCACCAGGCACCCCCGGACGGCCCGGATTTAACGGCCTGCCCGGAA ACCCCGGTGTACAAGGTCAGAAAGGCGAGCCAGGCGTGGGACTGCCTGGACTGAAAGGGCTGCCCGGCCT GCCTGGAATTCCCGGTACCCCCGGCGAGAAGGGAAGTATCGGTGTGCCCGGGGTGCCAGGAGAACATGGC GCGATTGGGCCCCCGGGACTGCAGGGGATTCGCGGCGAGCCCGGCCCCCCAGGGCTGCCCGGGAGCGTCG GCAGTCCTGGGGTGCCCGGCATCGGGCCACCCGGGGCCAGAGGACCGCCTGGGGGACAGGGCCCCCCGGG CCTTAGCGGCCCCCCAGGAATCAAAGGCGAAAAGGGATTCCCCGGCTTTCCAGGACTTGATATGCCTGGG CCTAAAGGGGACAAAGGGGCACAGGGGCTTCCAGGAATCACTGGGCAATCTGGACTCCCCGGGCTCCCCG GTCAGCAGGGCGCACCCGGCATTCCGGGATTCCCTGGCAGCAAGGGAGAAATGGGCGTTATGGGGACCCC CGGTCAGCCAGGCTCACCCGGACCCGTGGGTGCACCCGGACTTCCAGGAGAAAAGGGAGATCATGGCTTT CCAGGCTCCAGCGGCCCACGGGGCGACCCAGGACTGAAAGGAGATAAGGGAGATGTGGGACTGCCTGGCA AGCCTGGCTCCATGGACAAGGTAGACATGGGGAGCATGAAGGGCCAGAAGGGCGATCAGGGAGAGAAGGG ACAAATTGGTCCTATCGGTGAAAAGGGCAGTAGGGGGGATCCTGGTACACCCGGAGTGCCAGGAAAAGAT GGGCAGGCAGGACAGCCTGGACAGCCCGGGCCCAAGGGAGATCCCGGAATCTCTGGGACCCCTGGAGCTC CCGGACTCCCCGGACCCAAGGGATCAGTAGGGGGCATGGGCTTGCCTGGAACCCCCGGCGAGAAAGGGGT GCCGGGGATCCCGGGCCCTCAAGGATCTCCAGGGCTTCCTGGCGATAAAGGGGCAAAAGGGGAAAAAGGC CAAGCCGGCCCACCTGGAATTGGAATCCCTGGCCTTCGAGGAGAGAAGGGCGACCAAGGAATAGCAGGCT TTCCCGGGTCCCCGGGCGAAAAAGGGGAGAAAGGAAGTATCGGAATTCCAGGCATGCCTGGTTCTCCAGG TCTGAAGGGATCACCCGGCTCAGTCGGTTATCCTGGCAGTCCCGGGTTGCCAGGCGAGAAGGGCGATAAA GGACTTCCAGGCCTGGATGGAATACCCGGTGTCAAGGGAGAGGCAGGCCTCCCTGGTACTCCCGGGCCCA CCGGCCCCGCCGGACAAAAAGGCGAACCCGGTTCCGATGGAATTCCAGGGTCTGCGGGGGAGAAGGGGGA ACCCGGGCTCCCCGGGCGCGGATTCCCGGGGTTTCCCGGGGCCAAAGGAGACAAGGGATCCAAAGGGGAG GTAGGGTTCCCTGGTCTGGCCGGCTCCCCGGGCATTCCAGGAAGTAAAGGGGAGCAAGGTTTTATGGGGC CCCCCGGGCCTCAGGGTCAACCAGGGCTGCCTGGTAGTCCCGGCCACGCCACCGAGGGCCCCAAGGGCGA CAGGGGGCCGCAGGGTCAGCCAGGTCTGCCAGGACTGCCAGGCCCAATGGGCCCGCCTGGCCTCCCTGGC ATAGATGGGGTAAAAGGGGACAAAGGCAATCCCGGCTGGCCCGGCGCCCCTGGCGTGCCTGGACCGAAGG GAGATCCTGGTTTTCAAGGCATGCCCGGAATAGGGGGAAGCCCTGGCATCACCGGCAGCAAGGGAGACAT GGGACCGCCGGGCGTACCCGGCTTTCAGGGGCCCAAGGGCCTGCCCGGACTTCAGGGAATTAAGGGCGAT CAGGGCGACCAGGGAGTACCCGGCGCTAAGGGTCTTCCTGGGCCACCTGGTCCTCCAGGTCCCTATGATA TTATCAAAGGGGAACCAGGGTTGCCTGGGCCCGAGGGGCCTCCAGGCTTGAAAGGCCTCCAGGGACTGCC AGGACCCAAGGGACAACAGGGTGTGACCGGCCTCGTCGGCATCCCAGGACCCCCCGGCATCCCTGGATTC GACGGCGCTCCCGGCCAGAAGGGAGAAATGGGTCCTGCTGGACCTACAGGACCGCGCGGCTTCCCAGGAC CTCCGGGGCCAGATGGGTTGCCTGGTTCTATGGGGCCCCCTGGCACTCCTTCAGTTGACCACGGGTTCTT GGTGACTAGGCATAGCCAGACCATTGATGACCCACAGTGCCCCTCCGGCACCAAGATTTTGTACCACGGC TATAGCCTCCTCTATGTGCAAGGAAACGAGCGCGCGCATGGACAGGACCTGGGAACAGCCGGAAGTTGCC TGCGCAAGTTCAGCACCATGCCTTTCCTTTTTTGTAATATTAACAACGTATGCAATTTTGCCTCCCGGAA CGACTATTCCTACTGGTTGAGTACTCCGGAACCTATGCCCATGTCAATGGCCCCCATTACAGGCGAAAAC ATTCGACCCTTTATTTCAAGGTGTGCTGTCTGCGAAGCTCCAGCTATGGTGATGGCGGTGCACAGCCAAA CCATCCAAATCCCGCCCTGTCCATCAGGGTGGTCCAGCCTGTGGATTGGATATAGCTTTGTGATGCACAC GTCCGCAGGAGCTGAGGGCTCTGGCCAGGCCCTGGCAAGTCCAGGTAGCTGCTTGGAAGAGTTTCGATCA GCGCCTTTCATAGAATGTCATGGGCGGGGAACTTGCAACTACTACGCGAACGCTTATAGCTTTTGGTTGG CTACCATCGAGCGCAGCGAAATGTTTAAGAAGCCTACCCCTAGCACCCTCAAGGCTGGCGAGCTTCGGAC CCACGTGTCCCGATGCCAGGTCTGTATGCGCCGGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213796 representing NM_001845
Red=Cloning site Green=Tags(s) MGPRLSVWLLLLPAALLLHEEHSRAAAKGGCAGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEG PQGPPGQKGDTGEPGLPGTKGTRGPPGASGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGL PGFAGNPGPPGLPGMKGDPGEILGHVPGMLLKGERGFPGIPGTPGPPGLPGLQGPVGPPGFTGPPGPPGP PGPPGEKGQMGLSFQGPKGDKGDQGVSGPPGVPGQAQVQEKGDFATKGEKGQKGEPGFQGMPGVGEKGEP GKPGPRGKPGKDGDKGEKGSPGFPGEPGYPGLIGRQGPQGEKGEAGPPGPPGIVIGTGPLGEKGERGYPG TPGPRGEPGPKGFPGLPGQPGPPGLPVPGQAGAPGFPGERGEKGDRGFPGTSLPGPSGRDGLPGPPGSPG PPGQPGYTNGIVECQPGPPGDQGPPGIPGQPGFIGEIGEKGQKGESCLICDIDGYRGPPGPQGPPGEIGF PGQPGAKGDRGLPGRDGVAGVPGPQGTPGLIGQPGAKGEPGEFYFDLRLKGDKGDPGFPGQPGMPGRAGS PGRDGHPGLPGPKGSPGSVGLKGERGPPGGVGFPGSRGDTGPPGPPGYGPAGPIGDKGQAGFPGGPGSPG LPGPKGEPGKIVPLPGPPGAEGLPGSPGFPGPQGDRGFPGTPGRPGLPGEKGAVGQPGIGFPGPPGPKGV DGLPGDMGPPGTPGRPGFNGLPGNPGVQGQKGEPGVGLPGLKGLPGLPGIPGTPGEKGSIGVPGVPGEHG AIGPPGLQGIRGEPGPPGLPGSVGSPGVPGIGPPGARGPPGGQGPPGLSGPPGIKGEKGFPGFPGLDMPG PKGDKGAQGLPGITGQSGLPGLPGQQGAPGIPGFPGSKGEMGVMGTPGQPGSPGPVGAPGLPGEKGDHGF PGSSGPRGDPGLKGDKGDVGLPGKPGSMDKVDMGSMKGQKGDQGEKGQIGPIGEKGSRGDPGTPGVPGKD GQAGQPGQPGPKGDPGISGTPGAPGLPGPKGSVGGMGLPGTPGEKGVPGIPGPQGSPGLPGDKGAKGEKG QAGPPGIGIPGLRGEKGDQGIAGFPGSPGEKGEKGSIGIPGMPGSPGLKGSPGSVGYPGSPGLPGEKGDK GLPGLDGIPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEPGLPGRGFPGFPGAKGDKGSKGE VGFPGLAGSPGIPGSKGEQGFMGPPGPQGQPGLPGSPGHATEGPKGDRGPQGQPGLPGLPGPMGPPGLPG IDGVKGDKGNPGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGPPGVPGFQGPKGLPGLQGIKGD QGDQGVPGAKGLPGPPGPPGPYDIIKGEPGLPGPEGPPGLKGLQGLPGPKGQQGVTGLVGIPGPPGIPGF DGAPGQKGEMGPAGPTGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTIDDPQCPSGTKILYHG YSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPITGEN IRPFISRCAVCEAPAMVMAVHSQTIQIPPCPSGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRS APFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001845 |
ORF Size | 5007 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001845.1, NM_001845.2, NM_001845.3, NM_001845.4, NM_001845.5, NP_001836.1 |
RefSeq Size | 6540 bp |
RefSeq ORF | 5010 bp |
Locus ID | 1282 |
Cytogenetics | 13q34 |
Protein Pathways | ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer |
MW | 160.6 kDa |
Gene Summary | 'This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]' |
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