Collagen IV (COL4A1) (NM_001845) Human Tagged ORF Clone

CAT#: RG213796

COL4A1 (GFP-tagged) - Human collagen, type IV, alpha 1 (COL4A1). Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: COL4A1
• Synonyms: BSVD; BSVD1; RATOR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213796 ORF sequence, codon optimized.
  Due to the complexity of NM_001845, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCGAGACTGTCTGTATGGTTGCTCCTGCTGCCCGCTGCCCTGCTGCTTCACGAGGAGCATTCCC
  GGGCTGCCGCGAAAGGGGGGTGTGCAGGCAGCGGCTGTGGTAAATGTGATTGTCACGGCGTCAAGGGCCA
  GAAGGGAGAGCGGGGTCTGCCGGGTCTGCAAGGTGTGATCGGATTTCCCGGAATGCAGGGTCCAGAGGGC
  CCACAGGGCCCACCCGGCCAGAAAGGCGACACCGGAGAGCCAGGCCTGCCTGGCACCAAAGGAACACGGG
  GACCACCTGGGGCTAGCGGATACCCTGGAAACCCCGGGCTGCCGGGGATTCCCGGCCAGGACGGGCCCCC
  CGGTCCACCCGGAATTCCAGGGTGTAACGGTACCAAGGGAGAACGCGGCCCACTGGGTCCCCCGGGCTTG
  CCTGGGTTCGCAGGCAACCCGGGACCCCCTGGGCTGCCGGGGATGAAGGGAGATCCCGGCGAAATCCTTG
  GTCATGTTCCCGGGATGCTGCTCAAGGGCGAAAGGGGATTCCCAGGCATCCCGGGGACGCCCGGTCCGCC
  TGGACTGCCAGGTCTGCAGGGACCTGTGGGCCCACCCGGGTTTACTGGGCCCCCAGGGCCACCTGGACCA
  CCCGGACCCCCAGGGGAGAAGGGACAGATGGGGCTGTCTTTTCAGGGTCCGAAAGGGGACAAAGGGGACC
  AAGGCGTGAGTGGCCCTCCTGGAGTGCCCGGCCAGGCCCAGGTACAGGAGAAGGGCGATTTTGCTACTAA
  AGGCGAAAAGGGACAAAAGGGTGAACCTGGCTTTCAAGGGATGCCGGGGGTTGGAGAGAAGGGGGAGCCA
  GGAAAACCAGGCCCGAGAGGCAAGCCTGGGAAGGATGGAGACAAAGGAGAGAAAGGAAGCCCCGGCTTTC
  CCGGTGAACCAGGGTACCCAGGACTGATTGGGCGCCAGGGACCACAGGGTGAGAAAGGAGAAGCAGGCCC
  TCCTGGACCTCCAGGAATCGTCATTGGAACAGGACCATTGGGGGAGAAGGGCGAGAGAGGATACCCCGGC
  ACCCCAGGGCCGCGGGGCGAACCAGGGCCTAAGGGGTTTCCAGGGCTGCCAGGTCAGCCAGGTCCTCCAG
  GTCTGCCGGTGCCAGGCCAGGCCGGAGCTCCTGGCTTCCCTGGTGAGAGAGGCGAAAAAGGCGACAGAGG
  TTTTCCAGGCACTTCTTTGCCTGGCCCGTCCGGCAGGGATGGCCTGCCGGGACCACCGGGCAGTCCCGGG
  CCCCCCGGTCAGCCTGGATATACAAATGGCATCGTGGAGTGTCAACCAGGACCCCCCGGAGATCAAGGCC
  CACCCGGAATCCCAGGGCAGCCTGGATTCATTGGCGAGATTGGAGAGAAGGGGCAGAAAGGGGAATCATG
  CCTGATATGTGATATTGATGGTTACAGAGGGCCACCCGGGCCCCAGGGCCCCCCCGGGGAAATCGGCTTT
  CCAGGTCAGCCAGGTGCAAAGGGGGACAGGGGACTGCCAGGAAGGGACGGGGTAGCCGGCGTGCCAGGCC
  CCCAGGGCACCCCTGGACTCATTGGTCAGCCCGGTGCCAAGGGTGAACCTGGGGAATTCTATTTTGATCT
  GCGCCTCAAAGGCGACAAAGGGGACCCGGGCTTCCCAGGCCAGCCCGGAATGCCTGGCAGAGCCGGGAGC
  CCAGGGCGAGATGGACACCCAGGTCTGCCAGGACCCAAGGGCAGCCCAGGAAGCGTTGGCCTGAAAGGGG
  AACGCGGTCCTCCCGGGGGCGTGGGATTCCCGGGAAGCCGGGGTGATACAGGACCTCCTGGCCCTCCTGG
  ATATGGTCCAGCTGGCCCTATCGGGGATAAGGGGCAGGCAGGGTTCCCTGGAGGGCCTGGGAGCCCCGGG
  CTTCCTGGACCAAAAGGCGAGCCTGGAAAAATTGTCCCTCTTCCCGGGCCACCTGGAGCGGAGGGTCTGC
  CAGGCTCACCCGGCTTTCCGGGACCCCAGGGGGACCGGGGATTCCCTGGTACACCCGGCCGCCCCGGCCT
  GCCCGGAGAAAAAGGGGCAGTAGGACAGCCTGGGATCGGATTCCCGGGACCGCCTGGACCCAAGGGAGTC
  GATGGGCTGCCGGGCGATATGGGGCCACCAGGCACCCCCGGACGGCCCGGATTTAACGGCCTGCCCGGAA
  ACCCCGGTGTACAAGGTCAGAAAGGCGAGCCAGGCGTGGGACTGCCTGGACTGAAAGGGCTGCCCGGCCT
  GCCTGGAATTCCCGGTACCCCCGGCGAGAAGGGAAGTATCGGTGTGCCCGGGGTGCCAGGAGAACATGGC
  GCGATTGGGCCCCCGGGACTGCAGGGGATTCGCGGCGAGCCCGGCCCCCCAGGGCTGCCCGGGAGCGTCG
  GCAGTCCTGGGGTGCCCGGCATCGGGCCACCCGGGGCCAGAGGACCGCCTGGGGGACAGGGCCCCCCGGG
  CCTTAGCGGCCCCCCAGGAATCAAAGGCGAAAAGGGATTCCCCGGCTTTCCAGGACTTGATATGCCTGGG
  CCTAAAGGGGACAAAGGGGCACAGGGGCTTCCAGGAATCACTGGGCAATCTGGACTCCCCGGGCTCCCCG
  GTCAGCAGGGCGCACCCGGCATTCCGGGATTCCCTGGCAGCAAGGGAGAAATGGGCGTTATGGGGACCCC
  CGGTCAGCCAGGCTCACCCGGACCCGTGGGTGCACCCGGACTTCCAGGAGAAAAGGGAGATCATGGCTTT
  CCAGGCTCCAGCGGCCCACGGGGCGACCCAGGACTGAAAGGAGATAAGGGAGATGTGGGACTGCCTGGCA
  AGCCTGGCTCCATGGACAAGGTAGACATGGGGAGCATGAAGGGCCAGAAGGGCGATCAGGGAGAGAAGGG
  ACAAATTGGTCCTATCGGTGAAAAGGGCAGTAGGGGGGATCCTGGTACACCCGGAGTGCCAGGAAAAGAT
  GGGCAGGCAGGACAGCCTGGACAGCCCGGGCCCAAGGGAGATCCCGGAATCTCTGGGACCCCTGGAGCTC
  CCGGACTCCCCGGACCCAAGGGATCAGTAGGGGGCATGGGCTTGCCTGGAACCCCCGGCGAGAAAGGGGT
  GCCGGGGATCCCGGGCCCTCAAGGATCTCCAGGGCTTCCTGGCGATAAAGGGGCAAAAGGGGAAAAAGGC
  CAAGCCGGCCCACCTGGAATTGGAATCCCTGGCCTTCGAGGAGAGAAGGGCGACCAAGGAATAGCAGGCT
  TTCCCGGGTCCCCGGGCGAAAAAGGGGAGAAAGGAAGTATCGGAATTCCAGGCATGCCTGGTTCTCCAGG
  TCTGAAGGGATCACCCGGCTCAGTCGGTTATCCTGGCAGTCCCGGGTTGCCAGGCGAGAAGGGCGATAAA
  GGACTTCCAGGCCTGGATGGAATACCCGGTGTCAAGGGAGAGGCAGGCCTCCCTGGTACTCCCGGGCCCA
  CCGGCCCCGCCGGACAAAAAGGCGAACCCGGTTCCGATGGAATTCCAGGGTCTGCGGGGGAGAAGGGGGA
  ACCCGGGCTCCCCGGGCGCGGATTCCCGGGGTTTCCCGGGGCCAAAGGAGACAAGGGATCCAAAGGGGAG
  GTAGGGTTCCCTGGTCTGGCCGGCTCCCCGGGCATTCCAGGAAGTAAAGGGGAGCAAGGTTTTATGGGGC
  CCCCCGGGCCTCAGGGTCAACCAGGGCTGCCTGGTAGTCCCGGCCACGCCACCGAGGGCCCCAAGGGCGA
  CAGGGGGCCGCAGGGTCAGCCAGGTCTGCCAGGACTGCCAGGCCCAATGGGCCCGCCTGGCCTCCCTGGC
  ATAGATGGGGTAAAAGGGGACAAAGGCAATCCCGGCTGGCCCGGCGCCCCTGGCGTGCCTGGACCGAAGG
  GAGATCCTGGTTTTCAAGGCATGCCCGGAATAGGGGGAAGCCCTGGCATCACCGGCAGCAAGGGAGACAT
  GGGACCGCCGGGCGTACCCGGCTTTCAGGGGCCCAAGGGCCTGCCCGGACTTCAGGGAATTAAGGGCGAT
  CAGGGCGACCAGGGAGTACCCGGCGCTAAGGGTCTTCCTGGGCCACCTGGTCCTCCAGGTCCCTATGATA
  TTATCAAAGGGGAACCAGGGTTGCCTGGGCCCGAGGGGCCTCCAGGCTTGAAAGGCCTCCAGGGACTGCC
  AGGACCCAAGGGACAACAGGGTGTGACCGGCCTCGTCGGCATCCCAGGACCCCCCGGCATCCCTGGATTC
  GACGGCGCTCCCGGCCAGAAGGGAGAAATGGGTCCTGCTGGACCTACAGGACCGCGCGGCTTCCCAGGAC
  CTCCGGGGCCAGATGGGTTGCCTGGTTCTATGGGGCCCCCTGGCACTCCTTCAGTTGACCACGGGTTCTT
  GGTGACTAGGCATAGCCAGACCATTGATGACCCACAGTGCCCCTCCGGCACCAAGATTTTGTACCACGGC
  TATAGCCTCCTCTATGTGCAAGGAAACGAGCGCGCGCATGGACAGGACCTGGGAACAGCCGGAAGTTGCC
  TGCGCAAGTTCAGCACCATGCCTTTCCTTTTTTGTAATATTAACAACGTATGCAATTTTGCCTCCCGGAA
  CGACTATTCCTACTGGTTGAGTACTCCGGAACCTATGCCCATGTCAATGGCCCCCATTACAGGCGAAAAC
  ATTCGACCCTTTATTTCAAGGTGTGCTGTCTGCGAAGCTCCAGCTATGGTGATGGCGGTGCACAGCCAAA
  CCATCCAAATCCCGCCCTGTCCATCAGGGTGGTCCAGCCTGTGGATTGGATATAGCTTTGTGATGCACAC
  GTCCGCAGGAGCTGAGGGCTCTGGCCAGGCCCTGGCAAGTCCAGGTAGCTGCTTGGAAGAGTTTCGATCA
  GCGCCTTTCATAGAATGTCATGGGCGGGGAACTTGCAACTACTACGCGAACGCTTATAGCTTTTGGTTGG
  CTACCATCGAGCGCAGCGAAATGTTTAAGAAGCCTACCCCTAGCACCCTCAAGGCTGGCGAGCTTCGGAC
  CCACGTGTCCCGATGCCAGGTCTGTATGCGCCGGACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213796 representing NM_001845
  Red=Cloning site Green=Tags(s)
  
  MGPRLSVWLLLLPAALLLHEEHSRAAAKGGCAGSGCGKCDCHGVKGQKGERGLPGLQGVIGFPGMQGPEG
  PQGPPGQKGDTGEPGLPGTKGTRGPPGASGYPGNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGL
  PGFAGNPGPPGLPGMKGDPGEILGHVPGMLLKGERGFPGIPGTPGPPGLPGLQGPVGPPGFTGPPGPPGP
  PGPPGEKGQMGLSFQGPKGDKGDQGVSGPPGVPGQAQVQEKGDFATKGEKGQKGEPGFQGMPGVGEKGEP
  GKPGPRGKPGKDGDKGEKGSPGFPGEPGYPGLIGRQGPQGEKGEAGPPGPPGIVIGTGPLGEKGERGYPG
  TPGPRGEPGPKGFPGLPGQPGPPGLPVPGQAGAPGFPGERGEKGDRGFPGTSLPGPSGRDGLPGPPGSPG
  PPGQPGYTNGIVECQPGPPGDQGPPGIPGQPGFIGEIGEKGQKGESCLICDIDGYRGPPGPQGPPGEIGF
  PGQPGAKGDRGLPGRDGVAGVPGPQGTPGLIGQPGAKGEPGEFYFDLRLKGDKGDPGFPGQPGMPGRAGS
  PGRDGHPGLPGPKGSPGSVGLKGERGPPGGVGFPGSRGDTGPPGPPGYGPAGPIGDKGQAGFPGGPGSPG
  LPGPKGEPGKIVPLPGPPGAEGLPGSPGFPGPQGDRGFPGTPGRPGLPGEKGAVGQPGIGFPGPPGPKGV
  DGLPGDMGPPGTPGRPGFNGLPGNPGVQGQKGEPGVGLPGLKGLPGLPGIPGTPGEKGSIGVPGVPGEHG
  AIGPPGLQGIRGEPGPPGLPGSVGSPGVPGIGPPGARGPPGGQGPPGLSGPPGIKGEKGFPGFPGLDMPG
  PKGDKGAQGLPGITGQSGLPGLPGQQGAPGIPGFPGSKGEMGVMGTPGQPGSPGPVGAPGLPGEKGDHGF
  PGSSGPRGDPGLKGDKGDVGLPGKPGSMDKVDMGSMKGQKGDQGEKGQIGPIGEKGSRGDPGTPGVPGKD
  GQAGQPGQPGPKGDPGISGTPGAPGLPGPKGSVGGMGLPGTPGEKGVPGIPGPQGSPGLPGDKGAKGEKG
  QAGPPGIGIPGLRGEKGDQGIAGFPGSPGEKGEKGSIGIPGMPGSPGLKGSPGSVGYPGSPGLPGEKGDK
  GLPGLDGIPGVKGEAGLPGTPGPTGPAGQKGEPGSDGIPGSAGEKGEPGLPGRGFPGFPGAKGDKGSKGE
  VGFPGLAGSPGIPGSKGEQGFMGPPGPQGQPGLPGSPGHATEGPKGDRGPQGQPGLPGLPGPMGPPGLPG
  IDGVKGDKGNPGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGPPGVPGFQGPKGLPGLQGIKGD
  QGDQGVPGAKGLPGPPGPPGPYDIIKGEPGLPGPEGPPGLKGLQGLPGPKGQQGVTGLVGIPGPPGIPGF
  DGAPGQKGEMGPAGPTGPRGFPGPPGPDGLPGSMGPPGTPSVDHGFLVTRHSQTIDDPQCPSGTKILYHG
  YSLLYVQGNERAHGQDLGTAGSCLRKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPITGEN
  IRPFISRCAVCEAPAMVMAVHSQTIQIPPCPSGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRS
  APFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRRT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001845
• ORF Size: 5007 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001845.3, NP_001836.1
• RefSeq Size: 6540 bp
• RefSeq ORF: 5010 bp
• Locus ID: 1282
• Cytogenetics: 13q34
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• Gene Summary: 'This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]'