p114RhoGEF (ARHGEF18) (NM_015318) Human Tagged ORF Clone

CAT#: RC213956

ARHGEF18 (Myc-DDK-tagged)-Human Rho/Rac guanine nucleotide exchange factor (GEF) 18 (ARHGEF18), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGEF18
• Synonyms: P114-RhoGEF; p114RhoGEF; RP78; SA-RhoGEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213956 representing NM_015318
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTCTCTCAGAAAGGGGGTCCCCAGCCAACACCGAGCCCGGCTGGCCCTGGGACGCAACTCGGAC
  CAATCACAGGAGAGATGGATGAAGCCGATTCTGCGTTTTTAAAATTTAAGCAGACAGCTGATGACTCTCT
  GTCCCTTACATCTCCAAACACCGAGTCCATTTTTGTAGAAGATCCCTACACCGCCTCGCTGAGGAGTGAG
  ATTGAGTCAGACGGCCACGAGTTTGAAGCTGAGTCCTGGAGCCTCGCCGTGGATGCAGCCTACGCCAAGA
  AGCAAAAGAGGGAGGTGGTGAAAAGACAAGATGTCCTTTATGAGCTGATGCAGACAGAGGTGCACCACGT
  GCGGACGCTCAAGATCATGCTGAAGGTGTACTCCAGGGCCCTGCAGGAGGAGCTGCAGTTCAGCAGCAAG
  GCCATTGGCCGCCTCTTCCCATGCGCTGACGACCTGCTGGAGACGCACAGCCACTTCCTCGCTCGGCTCA
  AGGAGCGCCGCCAGGAGTCCCTGGAGGAGGGCAGTGACCGGAATTATGTCATCCAGAAAATCGGCGACCT
  CCTGGTTCAGCAGTTTTCAGGTGAAAATGGGGAGAGAATGAAAGAAAAGTACGGTGTGTTTTGTAGTGGC
  CACAATGAAGCTGTTAGTCATTACAAGTTGCTGCTTCAGCAAAACAAGAAATTTCAAAACTTGATCAAGA
  AAATTGGCAACTTCTCCATCGTGCGGCGGCTTGGCGTGCAGGAGTGCATTCTCCTGGTTACACAACGCAT
  AACCAAATACCCAGTGCTGGTGGAGCGCATCATCCAGAACACGGAAGCTGGCACTGAGGACTATGAAGAC
  CTGACCCAGGCCTTGAACCTCATCAAAGATATCATCTCACAAGTGGACGCCAAGGTCAGTGAGTGTGAGA
  AGGGCCAGCGCCTCAGGGAGATCGCAGGGAAGATGGACCTGAAGTCTTCCAGCAAACTCAAGAACGGGCT
  CACCTTCCGCAAGGAAGACATGCTTCAGCGGCAGCTCCACCTGGAGGGCATGCTATGCTGGAAGACCACA
  TCAGGGCGCTTGAAAGATATCCTGGCTATCCTGCTGACCGACGTACTTTTGCTGCTACAAGAAAAAGATC
  AGAAATACGTCTTTGCTTCTGTGGACTCAAAGCCACCCGTCATCTCGTTACAAAAGCTCATCGTGAGGGA
  AGTGGCCAACGAGGAGAAAGCGATGTTTCTGATCAGCGCCTCCTTGCAAGGGCCGGAGATGTATGAAATC
  TACACGAGCTCCAAAGAGGACAGGAACGCCTGGATGGCCCACATCCAAAGGGCTGTGGAGAGCTGCCCTG
  ACGAGGAGGAGGGGCCCTTCAGCCTGCCCGAAGAGGAAAGGAAGGTGGTCGAGGCCCGCGCCACGAGACT
  CCGGGACTTTCAAGAGCGGTTGAGCATGAAAGACCAGCTGATCGCACAGAGCCTCCTAGAGAAACAGCAG
  ATCTACCTGGAGATGGCCGAGATGGGCGGCCTCGAAGACCTGCCCCAGCCCCGAGGCCTATTCCGTGGAG
  GGGACCCATCCGAGACCCTGCAGGGGGAGCTAATTCTCAAGTCGGCCATGAGCGAGATCGAGGGCATCCA
  GAGCCTGATCTGCAGGCAGCTGGGCAGCGCCAACGGCCAGGCGGAAGACGGAGGCAGCTCCACAGGCCCG
  CCCAGGAGGGCTGAGACCTTCGCGGGCTACGACTGCACAAACAGCCCCACCAAGAATGGCAGTTTCAAGA
  AGAAAGTCAGCAGCACTGACCCCAGGCCCCGAGACTGGCGAGGCCCCCCAAACAGCCCGGACTTGAAGCT
  CAGTGACAGTGACATTCCTGGGAGCTCTGAGGAATCGCCGCAGGTGGTGGAGGCGCCAGGCACGGAATCC
  GATCCCCGTCTGCCCACCGTCCTGGAGTCGGAGCTTGTCCAGCGGATCCAGACACTGTCCCAGCTGCTCC
  TGAACCTTCAGGCGGTAATCGCCCACCAGGACAGCTATGTGGAGACGCAGCGGGCTGCCATCCAGGAGCG
  GGAGAAGCAGTTCCGGCTGCAGTCGACGCGTGGGAACCTGCTGCTGGAGCAGGAGCGGCAACGCAACTTC
  GAGAAGCAGCGGGAGGAGCGCGCGGCCCTGGAGAAGCTGCAGAGCCAGCTGCGGCACGAGCAGCAGCGCT
  GGGAGCGCGAGCGCCAGTGGCAGCACCAGGAGCTGGAGCGTGCGGGCGCGCGGCTGCAGGAGCGCGAGGG
  CGAGGCGCGGCAGCTACGCGAGCGGCTGGAGCAGGAGCGGGCCGAGCTGGAGCGCCAGCGCCAGGCCTAC
  CAGCACGACCTGGAGCGGCTGCGCGAGGCCCAGCGTGCCGTGGAGCGCGAGCGGGAGCGCCTGGAGCTGC
  TGCGCCGCCTCAAGAAGCAGAACACCGCGCCAGGCGCGCTGCCGCCCGACACACTGGCCGAGGCCCAGCC
  CCCAAGCCACCCTCCCAGCTTCAACGGGGAAGGGCTGGAGGGCCCTCGTGTGAGCATGCTGCCATCCGGC
  GTGGGGCCAGAGTACGCAGAGCGCCCCGAGGTGGCTCGCCGGGACAGCGCCCCCACCGAGAACCGGCTGG
  CCAAGAGCGATGTGCCCATCCAGCTGCTCAGCGCCACCAACCAGTTCCAGAGGCAGGCGGCCGTGCAGCA
  GCAGATCCCCACCAAGCTGGCGGCCTCCACCAAGGGTGGCAAGGACAAGGGCGGCAAGAGCAGGGGCTCT
  CAGCGCTGGGAGAGCTCAGCGTCCTTCGACCTGAAGCAGCAGCTGCTGCTCAACAAGCTCATGGGGAAAG
  ATGAGAGCACCTCACGGAACCGCCGCTCGCTGAGCCCTATCCTGCCCGGCAGACACAGTCCTGCGCCCCC
  ACCAGACCCTGGCTTCCCCGCCCCGAGCCCACCGCCAGCTGACAGCCCCTCCGAGGGCTTCTCTCTCAAG
  GCCGGGGGCACAGCCCTCCTGCCCGGGCCCCCAGCTCCCTCGCCACTGCCGGCCACACCACTCAGCGCCA
  AGGAGGACGCCAGCAAAGAAGACGTCATCTTCTTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213956 representing NM_015318
  Red=Cloning site Green=Tags(s)
  
  MTVSQKGGPQPTPSPAGPGTQLGPITGEMDEADSAFLKFKQTADDSLSLTSPNTESIFVEDPYTASLRSE
  IESDGHEFEAESWSLAVDAAYAKKQKREVVKRQDVLYELMQTEVHHVRTLKIMLKVYSRALQEELQFSSK
  AIGRLFPCADDLLETHSHFLARLKERRQESLEEGSDRNYVIQKIGDLLVQQFSGENGERMKEKYGVFCSG
  HNEAVSHYKLLLQQNKKFQNLIKKIGNFSIVRRLGVQECILLVTQRITKYPVLVERIIQNTEAGTEDYED
  LTQALNLIKDIISQVDAKVSECEKGQRLREIAGKMDLKSSSKLKNGLTFRKEDMLQRQLHLEGMLCWKTT
  SGRLKDILAILLTDVLLLLQEKDQKYVFASVDSKPPVISLQKLIVREVANEEKAMFLISASLQGPEMYEI
  YTSSKEDRNAWMAHIQRAVESCPDEEEGPFSLPEEERKVVEARATRLRDFQERLSMKDQLIAQSLLEKQQ
  IYLEMAEMGGLEDLPQPRGLFRGGDPSETLQGELILKSAMSEIEGIQSLICRQLGSANGQAEDGGSSTGP
  PRRAETFAGYDCTNSPTKNGSFKKKVSSTDPRPRDWRGPPNSPDLKLSDSDIPGSSEESPQVVEAPGTES
  DPRLPTVLESELVQRIQTLSQLLLNLQAVIAHQDSYVETQRAAIQEREKQFRLQSTRGNLLLEQERQRNF
  EKQREERAALEKLQSQLRHEQQRWERERQWQHQELERAGARLQEREGEARQLRERLEQERAELERQRQAY
  QHDLERLREAQRAVERERERLELLRRLKKQNTAPGALPPDTLAEAQPPSHPPSFNGEGLEGPRVSMLPSG
  VGPEYAERPEVARRDSAPTENRLAKSDVPIQLLSATNQFQRQAAVQQQIPTKLAASTKGGKDKGGKSRGS
  QRWESSASFDLKQQLLLNKLMGKDESTSRNRRSLSPILPGRHSPAPPPDPGFPAPSPPPADSPSEGFSLK
  AGGTALLPGPPAPSPLPATPLSAKEDASKEDVIFF
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015318
• ORF Size: 3045 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015318.1, NM_015318.2, NM_015318.3, NP_056133.2
• RefSeq Size: 5252
• RefSeq ORF: 3048
• Locus ID: 23370
• Domains: RhoGEF, PH
• MW: 114.1 kDa
• Gene Summary: Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GEF family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Nov 2018]