HERC3 (NM_014606) Human Tagged ORF Clone

CAT#: RC214034

HERC3 (Myc-DDK-tagged)-Human hect domain and RLD 3 (HERC3)

Product Images

Western blot validation of overexpression lysate (Cat# LY415160) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214034 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HERC3
• Synonyms: KIAA0032
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214034 representing NM_014606
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTATGTTGGGGATATTGGTCTCTGGGCCAACCTGGTATCAGCACCAACCTGCAGGGAATTGTGGCTG
  AGCCCCAGGTGTGTGGGTTCATATCTGACAGAAGTGTCAAGGAAGTGGCCTGTGGGGGAAACCACTCTGT
  GTTCCTGCTGGAAGATGGGGAAGTTTACACATGTGGTTTGAACACCAAGGGGCAACTGGGCCATGAGAGG
  GAAGGAAACAAGCCAGAACAAATTGGAGCTCTGGCAGATCAGCATATCATTCATGTGGCATGTGGCGAGT
  CCCACAGTCTGGCCCTCAGTGACCGAGGCCAGCTGTTTTCTTGGGGTGCAGGGAGTGATGGTCAGCTAGG
  ACTCATGACTACTGAGGATTCTGTGGCAGTGCCCAGGTTAATACAAAAGCTGAACCAGCAAACAATATTA
  CAAGTTTCCTGTGGCAACTGGCATTGCTTGGCTCTTGCGGCTGATGGCCAGTTCTTCACCTGGGGAAAGA
  ACAGCCATGGGCAGCTTGGCTTAGGGAAGGAGTTCCCCTCCCAAGCCAGCCCACAGAGGGTGAGGTCCCT
  GGAGGGGATCCCACTGGCTCAGGTGGCTGCCGGAGGGGCTCACAGCTTTGCCCTGTCTCTCTCAGGAGCT
  GTTTTTGGCTGGGGGATGAATAATGCCGGGCAGCTAGGGCTCAGTGATGAAAAAGATCGAGAATCTCCAT
  GCCATGTAAAACTCTTACGCACGCAAAAAGTTGTCTATATTAGTTGTGGAGAAGAACACACAGCAGTTCT
  CACAAAGAGTGGAGGTGTGTTTACCTTTGGCGCTGGTTCCTGTGGGCAACTTGGACACGACTCCATGAAT
  GATGAGGTTAACCCTAGAAGAGTTCTAGAGCTGATGGGTAGTGAAGTAACTCAAATTGCTTGTGGCAGAC
  AACATACCCTAGCCTTCGTGCCTTCTTCTGGACTCATCTATGCATTTGGTTGTGGAGCAAGAGGTCAATT
  AGGAACTGGGCACACTTGTAATGTTAAGTGCCCATCTCCTGTCAAGGGTTACTGGGCTGCCCACAGTGGC
  CAGCTTTCAGCCCGAGCTGATCGCTTTAAATATCATATCGTTAAGCAGATCTTCTCTGGAGGAGACCAGA
  CTTTTGTACTTTGCTCCAAATACGAGAATTATTCTCCTGCTGTTGACTTCAGGACTATGAACCAAGCACA
  TTATACCAGTTTAATAAATGATGAAACCATAGCAGTTTGGAGACAAAAACTCTCAGAACACAACAATGCA
  AATACAATCAATGGTGTTGTTCAGATATTATCTTCTGCAGCCTGTTGGAATGGAAGTTTTCTTGAAAAAA
  AAATTGATGAACATTTTAAAACGAGTCCCAAAATCCCTGGGATTGACCTGAACTCAACTAGGGTGTTATT
  TGAGAAGTTAATGAACTCTCAGCACTCCATGATTCTAGAACAGATTTTGAACAGTTTTGAAAGTTGTCTG
  ATTCCCCAGTTGTCAAGCTCACCACCAGATGTTGAAGCCATGAGAATCTATTTAATACTACCTGAGTTTC
  CCCTACTCCAGGATTCCAAGTATTATATAACATTGACTATTCCCTTGGCTATGGCCATTCTTCGGCTGGA
  TACAAACCCCAGCAAAGTACTAGATAACTGGTGGTCTCAGGTATGCCCGAAATATTTCATGAAGCTGGTA
  AACCTCTATAAAGGTGCAGTCCTTTATCTACTGAGGGGAAGAAAGACATTCTTAATTCCCGTACTGTTTA
  ACAATTATATCACAGCAGCTCTCAAACTCTTGGAGAAGTTATATAAGGTAAATCTTAAAGTGAAGCATGT
  GGAATATGATACATTTTACATTCCTGAGATTTCCAATCTCGTGGACATTCAGGAAGACTACCTCATGTGG
  TTCTTGCATCAAGCAGGGATGAAGGCTAGACCATCAATAATACAGGATACTGTAACACTTTGTTCCTACC
  CTTTCATCTTTGATGCCCAAGCCAAGACCAAAATGTTACAGACAGATGCTGAACTACAGATGCAGGTGGC
  AGTCAATGGAGCCAACCTGCAGAATGTCTTCATGCTTCTCACCCTGGAGCCTCTGCTGGCCAGAAGCCCC
  TTCCTGGTCCTTCACGTTCGCAGGAACAACCTTGTTGGAGATGCCCTAAGAGAGCTGAGCATTCATTCTG
  ATATTGATTTGAAAAAGCCTCTCAAAGTAATCTTTGATGGTGAAGAAGCAGTGGATGCCGGTGGTGTTAC
  AAAGGAATTTTTTCTTTTGCTGTTAAAAGAACTTTTGAATCCCATCTATGGAATGTTTACCTACTATCAA
  GATTCAAATCTCTTGTGGTTTTCAGACACGTGTTTTGTAGAGCACAACTGGTTTCACTTGATTGGTATAA
  CCTGTGGACTAGCTATCTACAACTCCACTGTGGTCGATCTCCACTTCCCATTGGCTCTCTACAAGAAGTT
  ACTCAATGTAAAGCCTGGCTTGGAAGACTTAAAGGAGTTGTCACCCACTGAAGGAAGGAGTCTCCAAGAG
  CTTTTGGATTACCCCGGGGAGGATGTGGAGGAGACTTTCTGCCTCAACTTCACGATCTGCCGAGAAAGCT
  ATGGAGTGATTGAACAGAAGAAGCTGATACCTGGGGGAGATAATGTAACTGTGTGCAAGGATAACAGGCA
  GGAATTTGTGGATGCTTATGTGAATTATGTCTTCCAAATCTCAGTTCATGAATGGTACACAGCCTTCTCT
  AGTGGCTTCCTAAAGGTGTGTGGTGGCAAAGTACTTGAGCTCTTCCAGCCTTCAGAACTGAGGGCTATGA
  TGGTGGGGAACAGCAACTACAACTGGGAAGAACTGCAAGAGACTGCCATCTACAAGGGAGATTACTCGGC
  CACACATCCCACTGTAAAACTATTTTGGGAAACATTTCATGAGTTTCCATTGGAAAAGAAGAAGAAGTTT
  CTCTTGTTCCTGACAGGCAGCGATCGGATTCCCATCTACGGCATGGCCAGTCTGCAGATTGTCATCCAGT
  CCACAGCCAGCGGGGAGGAGTACTTGCCGGTGGCCCACACTTGCTACAACCTTCTTGACCTCCCCAAGTA
  CAGCAGCAAAGAGATTCTGAGTGCCCGGCTGACCCAGGCCCTTGACAACTATGAAGGGTTTAGTTTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214034 representing NM_014606
  Red=Cloning site Green=Tags(s)
  
  MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHER
  EGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWGAGSDGQLGLMTTEDSVAVPRLIQKLNQQTIL
  QVSCGNWHCLALAADGQFFTWGKNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGAHSFALSLSGA
  VFGWGMNNAGQLGLSDEKDRESPCHVKLLRTQKVVYISCGEEHTAVLTKSGGVFTFGAGSCGQLGHDSMN
  DEVNPRRVLELMGSEVTQIACGRQHTLAFVPSSGLIYAFGCGARGQLGTGHTCNVKCPSPVKGYWAAHSG
  QLSARADRFKYHIVKQIFSGGDQTFVLCSKYENYSPAVDFRTMNQAHYTSLINDETIAVWRQKLSEHNNA
  NTINGVVQILSSAACWNGSFLEKKIDEHFKTSPKIPGIDLNSTRVLFEKLMNSQHSMILEQILNSFESCL
  IPQLSSSPPDVEAMRIYLILPEFPLLQDSKYYITLTIPLAMAILRLDTNPSKVLDNWWSQVCPKYFMKLV
  NLYKGAVLYLLRGRKTFLIPVLFNNYITAALKLLEKLYKVNLKVKHVEYDTFYIPEISNLVDIQEDYLMW
  FLHQAGMKARPSIIQDTVTLCSYPFIFDAQAKTKMLQTDAELQMQVAVNGANLQNVFMLLTLEPLLARSP
  FLVLHVRRNNLVGDALRELSIHSDIDLKKPLKVIFDGEEAVDAGGVTKEFFLLLLKELLNPIYGMFTYYQ
  DSNLLWFSDTCFVEHNWFHLIGITCGLAIYNSTVVDLHFPLALYKKLLNVKPGLEDLKELSPTEGRSLQE
  LLDYPGEDVEETFCLNFTICRESYGVIEQKKLIPGGDNVTVCKDNRQEFVDAYVNYVFQISVHEWYTAFS
  SGFLKVCGGKVLELFQPSELRAMMVGNSNYNWEELQETAIYKGDYSATHPTVKLFWETFHEFPLEKKKKF
  LLFLTGSDRIPIYGMASLQIVIQSTASGEEYLPVAHTCYNLLDLPKYSSKEILSARLTQALDNYEGFSLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014606
• ORF Size: 3150 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014606.1, NM_014606.2, NP_055421.1
• RefSeq Size: 4894 bp
• RefSeq ORF: 3153 bp
• Locus ID: 8916
• Domains: RCC1, HECT
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 117 kDa
• Gene Summary: This gene encodes a member the HERC ubiquitin ligase family. The encoded protein is located in the cytosol and binds ubiquitin via a HECT domain. Mutations in this gene have been associated with colorectal and gastric carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2012]