EMILIN2 (NM_032048) Human Tagged ORF Clone

CAT#: RC214038

EMILIN2 (Myc-DDK-tagged)-Human elastin microfibril interfacer 2 (EMILIN2)

Product Images

Western blot validation of overexpression lysate (Cat# LY410362) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214038 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EMILIN2 protein (Cat# TP314038). The protein was produced from HEK293T cells transfected with EMILIN2 cDNA clone (Cat# RC214038) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EMILIN2
• Synonyms: EMILIN-2; FOAP-10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214038 representing NM_032048
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCAGCCCAGACGGCCCTGGCCCCGCGTGCCCTGGCGCTGGGCGCTGGCGCTGCTGGCCCTGGTTG
  GCGCGGGGCTGTGCCACGCCGGCCCGCAGCCCGGGTATCCCGCGCGGCCCAGCGCCAGGAACAAGAACTG
  GTGCGCCTACATCGTGAACAAGAATGTGAGCTGCTCCGTGCTGGAGGGAAGTGAGAGTTTTATTCAGGCT
  CAGTACAACTGTGCCTGGAACCAGATGCCCTGTCCGTCGGCGCTGGTGTATCGAGTGAACTTCAGACCTA
  GATATGTCACTAGGTATAAGACAGTGACACAGTTGGAATGGAGGTGCTGTCCTGGCTTTAGAGGGGGAGA
  TTGCCAAGAAGGTCCCAAAGACCCCGTGAAGACCCTCCGCCCCACGCCGGCTCGGCCTCGAAATAGCTTG
  AAGAAAGCCACAGATAATGAACCCAGCCAATTCTCAGAGCCCAGGAAGACTTTGTCCCCAACTGGTACAG
  CACAACCAAGCTGGGGGGTAGATCCAAAAGAGGGGCCTCAGGAACTTCAGGAAAAGAAGATACAGGTGCT
  AGAGGAGAAGGTTCTTCGACTCACAAGGACGGTTCTTGACCTCCAGTCTTCCCTTGCTGGAGTGAGTGAA
  AATCTCAAACATGCCACTCAGGATGATGCCAGTAGAACACGGGCACCAGGGCTCAGCAGCCAGCACCCCA
  AGCCTGACACCACTGTTAGTGGAGACACAGAAACGGGCCAGAGTCCTGGTGTCTTCAACACTAAGGAATC
  TGGCATGAAGGACATCAAGTCTGAATTGGCTGAAGTCAAAGATACTCTAAAGAACAAAAGTGACAAGCTG
  GAAGAGCTGGATGGAAAAGTGAAGGGCTACGAAGGGCAGCTCAGACAGCTCCAGGAAGCAGCTCAGGGCC
  CGACGGTGACCATGACAACCAACGAACTCTACCAAGCCTATGTGGACAGTAAGATCGACGCCCTGAGAGA
  GGAGCTCATGGAGGGCATGGACAGAAAGCTGGCTGACCTGAAAAACTCATGTGAGTACAAGCTCACTGGC
  CTCCAGCAGCAGTGTGATGACTATGGGAGCAGCTACCTGGGAGTGATAGAGCTCATAGGGGAGAAGGAAA
  CAAGCCTGAGAAAAGAAATAAATAACCTCCGAGCCCGGCTACAGGAGCCTTCAGCCCAGGCAAATTGCTG
  CGACAGTGAAAAGAATGGTGACATTGGTCAACAGATCAAGACATTGGACCAGAAAATCGAGAGAGTTGCT
  GAAGCCACCAGAATGCTGAATGGAAGACTGGACAATGAGTTTGACCGCCTTATAGTTCCAGAGCCAGATG
  TGGATTTTGATGCAAAATGGAATGAACTCGATGCAAGGATCAATGTGACGGAGAAGAACGCTGAAGAACA
  TTGCTTTTACATTGAGGAAACCCTTCGGGGCGCCATTAATGGAGAGGTGGGTGACTTGAAGCAGCTTGTT
  GATCAGAAAATACAGTCTCTGGAAGACCGTCTGGGGAGCGTTCTCCTACAGATGACCAATAACACTGGTG
  CAGAGCTCAGTCCCCCAGGGGCAGCAGCCCTGCCAGGAGTGTCAGGGTCAGGAGATGAACGGGTCATGAT
  GGAATTAAACCACCTGAAGGACAAAGTTCAAGTTGTTGAAGACATTTGCCTGCTGAACATCCAGGGAAAG
  CCTCATGGGATGGAAGGTGCCTTGCCAAACAGGGAAGACCGCGCAGTACGCGACAGCCTGCACCTTTTGA
  AATCTCTCAACGACACGATGCACAGGAAGTTTCAAGAAACCGAACAAACCATCCAGAAACTTCAACAGGA
  TTTTAGTTTTCTTTATTCTCAATTAAACCACACAGAAAATGATGTGACTCATCTTCAAAAGGAAATGAGC
  AATTGTAGAGCAGGTGAAAACGCTGGCATGGGTAGGTTCACTAAGGTGGGTGAGCAAGAAAGGACAGTGG
  ACACCCTGCCGTCCCCCCAGCACCCCGTGGCTCATTGCTGCAGTCAGCTGGAGGAGAGGTGGCAGAGGTT
  GCAGAGCCAGGTCATCTCGGAGCTGGATGCTTGTAAGGAATGCACGCAGGGGGTCCAGAGGGAGGTCTCC
  ATGGTGGAGGGCAGGGTGTCTCATATGGAGAAAACTTGCAGCAAGCTGGACTCTATCTCAGGAAATCTTC
  AGAGGATCAAGGAGGGGCTCAACAAGCATGTCAGCAGCCTGTGGAACTGTGTCAGGCAGATGAACGGAAC
  GCTCAGGTCGCATTCCAGAGACATTTCTGGCCTGAAGAATTCAGTCCAGCAGTTCTACAGCCACGTCTTC
  CAGATTTCTACTGATTTGCAAGATCTGGTCAAATTTCAGCCATCAGCAAAGGCACCCTCGCCCCCGCCGC
  CCGCAGAGGCCCCGAAGGAGCCGCTGCAGCCCGAGCCCGCCCCGCCGAGGCCCAGCGGCCCCGCAACCGC
  AGAGGACCCTGGGCGACGGCCCGTCCTGCCCCAGCGGCCCCCCGAGGAGAGGCCGCCCCAGCCGCCAGGC
  TCCACCGGGGTCATCGCGGAGACGGGCCAGGCCGGGCCCCCCGCAGGCGCAGGCGTGTCTGGGCGGGGTC
  TGCCGCGGGGCGTGGACGGCCAGACCGGGAGCGGCACCGTCCCCGGCGCAGAAGGCTTCGCGGGCGCACC
  AGGATACCCGAAGTCACCTCCTGTAGCTTCCCCAGGAGCTCCGGTGCCTTCTCTGGTGTCTTTTTCTGCG
  GGGCTCACCCAGAAGCCTTTCCCCAGTGATGGGGGCGTTGTCCTCTTTAACAAAGTGCTGGTGAACGACG
  GGGATGTTTACAACCCCAGCACCGGGGTCTTCACGGCTCCTTATGATGGGCGCTACCTGATCACGGCCAC
  CCTCACCCCCGAGAGAGACGCCTACGTGGAAGCAGTGCTGTCGGTCTCCAACGCCAGCGTGGCCCAGCTG
  CATACCGCTGGGTACAGGAGAGAGTTCCTGGAATACCACCGCCCTCCAGGAGCTTTGCATACCTGCGGGG
  GCCCGGGGGCATTCCACCTCATCGTGCACCTGAAGGCGGGAGATGCAGTCAACGTCGTGGTGACTGGGGG
  CAAGCTGGCTCACACAGACTTTGATGAAATGTACTCCACATTTAGTGGGGTTTTCTTATATCCTTTCCTT
  TCCCACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214038 representing NM_032048
  Red=Cloning site Green=Tags(s)
  
  MWQPRRPWPRVPWRWALALLALVGAGLCHAGPQPGYPARPSARNKNWCAYIVNKNVSCSVLEGSESFIQA
  QYNCAWNQMPCPSALVYRVNFRPRYVTRYKTVTQLEWRCCPGFRGGDCQEGPKDPVKTLRPTPARPRNSL
  KKATDNEPSQFSEPRKTLSPTGTAQPSWGVDPKEGPQELQEKKIQVLEEKVLRLTRTVLDLQSSLAGVSE
  NLKHATQDDASRTRAPGLSSQHPKPDTTVSGDTETGQSPGVFNTKESGMKDIKSELAEVKDTLKNKSDKL
  EELDGKVKGYEGQLRQLQEAAQGPTVTMTTNELYQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLTG
  LQQQCDDYGSSYLGVIELIGEKETSLRKEINNLRARLQEPSAQANCCDSEKNGDIGQQIKTLDQKIERVA
  EATRMLNGRLDNEFDRLIVPEPDVDFDAKWNELDARINVTEKNAEEHCFYIEETLRGAINGEVGDLKQLV
  DQKIQSLEDRLGSVLLQMTNNTGAELSPPGAAALPGVSGSGDERVMMELNHLKDKVQVVEDICLLNIQGK
  PHGMEGALPNREDRAVRDSLHLLKSLNDTMHRKFQETEQTIQKLQQDFSFLYSQLNHTENDVTHLQKEMS
  NCRAGENAGMGRFTKVGEQERTVDTLPSPQHPVAHCCSQLEERWQRLQSQVISELDACKECTQGVQREVS
  MVEGRVSHMEKTCSKLDSISGNLQRIKEGLNKHVSSLWNCVRQMNGTLRSHSRDISGLKNSVQQFYSHVF
  QISTDLQDLVKFQPSAKAPSPPPPAEAPKEPLQPEPAPPRPSGPATAEDPGRRPVLPQRPPEERPPQPPG
  STGVIAETGQAGPPAGAGVSGRGLPRGVDGQTGSGTVPGAEGFAGAPGYPKSPPVASPGAPVPSLVSFSA
  GLTQKPFPSDGGVVLFNKVLVNDGDVYNPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVSNASVAQL
  HTAGYRREFLEYHRPPGALHTCGGPGAFHLIVHLKAGDAVNVVVTGGKLAHTDFDEMYSTFSGVFLYPFL
  SHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032048
• ORF Size: 3159 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032048.1, NM_032048.2, NP_114437.2
• RefSeq Size: 4009
• RefSeq ORF: 3162
• Locus ID: 84034
• MW: 115.5 kDa
• Gene Summary: May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity. [UniProtKB/Swiss-Prot Function]