TUT7 (NM_024617) Human Tagged ORF Clone

CAT#: RC214200

ZCCHC6 (Myc-DDK-tagged)-Human zinc finger, CCHC domain containing 6 (ZCCHC6), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY411206) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214200 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TUT7
• Synonyms: PAPD6; TENT3B; ZCCHC6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214200 representing NM_024617
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATACAGCAAAACCTTATTTCGTGAAGCGCACTAAAGACCGGGGGACTATGGATGATGATGACT
  TCAGAAGGGGTCACCCCCAACAAGATTATTTAATAATAGATGACCATGCTAAAGGCCATGGCAGTAAAAT
  GGAAAAGGGCCTTCAAAAAAAGAAGATAACACCAGGGAACTATGGGAATACCCCCAGAAAAGGACCATGT
  GCTGTTTCCAGCAATCCATATGCATTTAAAAACCCAATCTACAGTCAACCCGCTTGGATGAATGACAGCC
  ACAAAGATCAGAGTAAGAGATGGCTGTCTGATGAACATACTGGTAATTCAGACAACTGGAGAGAATTCAA
  ACCTGGACCTAGAATTCCTGTTATAAACCGACAAAGAAAAGACTCCTTTCAAGAAAATGAAGATGGTTAT
  AGGTGGCAAGACACAAGAGGCTGCAGAACTGTAAGACGACTGTTTCATAAAGACCTAACAAGCCTAGAAA
  CCACGTCAGAAATGGAAGCAGGAAGTCCTGAAAACAAGAAGCAGAGGTCCAGACCTAGGAAGCCACGGAA
  GACTAGAAATGAGGAAAATGAGCAGGATGGAGACTTGGAAGGCCCTGTGATCGATGAGTCTGTACTTTCA
  ACGAAGGAGCTGCTAGGCTTACAGCAGGCTGAGGAGAGACTGAAGAGAGACTGCATTGACAGGCTAAAAA
  GGCGACCACGAAACTACCCTACAGCAAAGTACACCTGCAGACTCTGTGATGTTTTAATTGAATCCATTGC
  ATTTGCCCATAAGCATATCAAGGAAAAGAGGCACAAGAAAAACATTAAGGAGAAGCAAGAGGAAGAGTTG
  CTCACTACGTTACCCCCACCAACACCCTCCCAGATAAATGCAGTTGGCATTGCCATTGACAAAGTGGTAC
  AGGAATTTGGCTTACACAATGAGAACTTGGAACAGAGGCTGGAAATTAAACGTATCATGGAAAATGTGTT
  CCAACACAAGTTACCAGATTGTTCCCTAAGATTATATGGGTCATCCTGTAGCAGATTGGGTTTCAAAAAT
  TCGGATGTAAACATTGACATCCAGTTTCCAGCCATTATGTCTCAGCCAGATGTCCTCTTACTTGTTCAAG
  AATGTTTAAAGAACAGTGACTCCTTTATTGATGTTGATGCAGACTTCCATGCTAGGGTGCCAGTGGTGGT
  GTGCAGAGAAAAGCAAAGTGGTCTTCTGTGTAAAGTGAGCGCAGGAAATGAAAATGCTTGTCTGACAACA
  AAGCATTTAACTGCCCTTGGAAAACTAGAACCAAAGCTGGTTCCTTTGGTGATTGCATTTAGGTACTGGG
  CAAAGCTTTGCAGTATAGATCGCCCTGAAGAAGGAGGTCTGCCACCTTATGTGTTTGCCCTGATGGCCAT
  TTTCTTTCTTCAGCAGAGGAAAGAACCCCTTTTGCCTGTATATCTAGGATCATGGATTGAAGGATTCTCA
  TTAAGCAAACTAGGGAATTTCAACCTTCAAGACATTGAAAAAGATGTTGTGATCTGGGAACATACTGACA
  GTGCTGCAGGGGACACAGGCATAACAAAAGAAGAGGCACCAAGAGAAACGCCGATTAAAAGGGGACAGGT
  GTCATTAATATTGGATGTGAAACACCAGCCTTCAGTACCAGTTGGGCAGCTCTGGGTGGAATTGCTGCGG
  TTCTATGCTTTAGAATTTAATTTGGCTGATTTAGTGATAAGTATTCGTGTCAAAGAATTGGTATCTCGGG
  AATTGAAGGATTGGCCCAAAAAGCGCATTGCCATTGAAGATCCCTACTCTGTTAAAAGAAATGTGGCAAG
  AACCCTAAATAGTCAACCTGTGTTTGAATATATACTTCATTGTTTAAGGACAACATACAAGTATTTTGCT
  CTTCCACACAAAATTACAAAATCCAGCCTTCTAAAGCCTCTGAATGCAATTACATGTATTTCAGAACATT
  CTAAAGAAGTAATAAATCATCATCCAGATGTACAAACAAAAGATGATAAGCTCAAAAACTCAGTTTTGGC
  CCAAGGTCCTGGTGCTACCAGTTCAGCTGCAAATACCTGTAAGGTACAGCCACTTACTCTTAAAGAGACT
  GCTGAAAGTTTTGGAAGCCCACCAAAAGAAGAAATGGGAAATGAACACATCAGTGTCCACCCTGAAAACT
  CAGACTGTATCCAAGCAGATGTTAACTCTGATGATTACAAGGGTGATAAAGTATACCATCCAGAAACAGG
  AAGGAAAAACGAGAAAGAGAAAGTTGGAAGGAAGGGCAAGCATCTGTTGACTGTTGATCAGAAACGTGGA
  GAGCATGTTGTCTGTGGCAGCACACGTAATAATGAGTCAGAGAGCACTTTGGATTTAGAAGGCTTCCAAA
  ATCCCACAGCTAAAGAGTGTGAGGGACTTGCCACTTTAGATAACAAGGCTGATCTTGATGGAGAAAGTAC
  AGAAGGTACTGAGGAACTAGAAGACTCTCTAAACCACTTTACCCACTCAGTACAGGGCCAGACATCAGAA
  ATGATTCCCTCTGATGAAGAGGAGGAGGACGACGAAGAAGAGGAGGAGGAAGAAGAACCTAGGCTCACCA
  TTAACCAAAGGGAAGATGAAGATGGCATGGCTAATGAAGATGAGTTAGACAACACCTACACTGGGTCAGG
  GGATGAGGACGCCCTATCTGAAGAGGATGATGAGTTAGGCGAAGCTGCTAAGTATGAAGACGTGAAAGAA
  TGTGGAAAACATGTAGAAAGAGCTCTCCTAGTGGAACTTAATAAAATAAGTCTCAAGGAAGAAAATGTAT
  GTGAAGAAAAAAATTCACCTGTGGATCAGTCTGATTTTTTTTATGAATTCAGTAAACTTATCTTCACCAA
  AGGCAAGTCTCCTACGGTAGTGTGCAGCTTATGCAAACGAGAGGGTCATCTAAAGAAGGACTGTCCTGAA
  GACTTCAAAAGAATCCAGCTAGAACCTCTGCCACCATTAACACCCAAGTTTTTAAATATCTTAGATCAAG
  TCTGTATCCAGTGTTATAAGGATTTTTCTCCAACAATTATAGAAGATCAGGCTCGTGAACATATTCGGCA
  AAACCTAGAAAGTTTCATAAGACAGGACTTTCCAGGAACTAAATTGAGCCTGTTTGGCTCCTCCAAAAAT
  GGATTTGGGTTCAAACAGAGTGACCTTGACGTCTGTATGACAATTAATGGACTTGAAACTGCTGAGGGAT
  TGGACTGTGTCAGAACTATTGAAGAATTAGCAAGAGTCCTCAGAAAACATTCAGGTCTGAGAAACATCTT
  ACCTATTACAACAGCAAAGGTGCCAATTGTGAAGTTCTTCCATTTGAGAAGTGGTCTGGAAGTAGATATC
  AGTTTGTATAACACATTGGCCCTTCATAACACAAGGCTTTTATCTGCTTATTCCGCCATTGATCCCAGAG
  TGAAGTATTTGTGCTATACCATGAAAGTATTTACAAAGATGTGTGATATTGGTGATGCATCTAGAGGCAG
  CTTATCATCGTATGCATATACTCTTATGGTGCTATATTTTCTCCAGCAGAGGAATCCACCAGTCATTCCT
  GTCCTTCAAGAGATATACAAAGGTGAAAAGAAACCTGAAATATTTGTTGATGGCTGGAATATTTATTTTT
  TTGATCAAATAGATGAACTGCCTACCTATTGGTCAGAATGTGGAAAAAATACAGAATCTGTTGGGCAGTT
  ATGGTTGGGCCTTCTTCGTTTCTACACAGAGGAATTTGATTTTAAAGAACATGTTATTAGCATCAGGAGA
  AAAAGTCTGCTTACAACTTTTAAGAAACAGTGGACCTCAAAATACATTGTTATTGAAGATCCCTTTGATT
  TGAATCATAATCTTGGAGCTGGATTATCAAGGAAAATGACAAATTTTATAATGAAGGCTTTTATCAATGG
  TAGAAGAGTATTTGGTATTCCTGTCAAGGGATTTCCAAAGGACTACCCCTCAAAAATGGAATACTTTTTT
  GATCCAGATGTGTTAACTGAAGGAGAGCTGGCCCCAAATGATAGATGTTGTCGAATTTGTGGAAAAATCG
  GACACTTCATGAAGGACTGTCCTATGAGGAGAAAAGTAAGACGGCGGCGAGATCAGGAAGATGCCCTGAA
  CCAAAGATACCCTGAGAACAAGGAAAAAAGAAGCAAAGAGGACAAAGAAATTCACAACAAGTACACAGAA
  AGGGAGGTGTCAACAAAAGAAGATAAGCCCATACAGTGCACACCTCAGAAAGCCAAGCCAATGCGGGCAG
  CTGCTGACCTGGGGAGGGAGAAGATCCTCAGGCCACCAGTAGAAAAATGGAAGAGACAGGATGACAAAGA
  CTTAAGAGAAAAACGTTGTTTTATTTGTGGAAGAGAAGGGCACATTAAAAAGGAATGCCCACAGTTTAAA
  GGCTCTTCAGGTAGCCTTTCCAGTAAATATATGACTCAGGGAAAAGCCTCAGCGAAGAGGACCCAGCAGG
  AATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214200 representing NM_024617
  Red=Cloning site Green=Tags(s)
  
  MGDTAKPYFVKRTKDRGTMDDDDFRRGHPQQDYLIIDDHAKGHGSKMEKGLQKKKITPGNYGNTPRKGPC
  AVSSNPYAFKNPIYSQPAWMNDSHKDQSKRWLSDEHTGNSDNWREFKPGPRIPVINRQRKDSFQENEDGY
  RWQDTRGCRTVRRLFHKDLTSLETTSEMEAGSPENKKQRSRPRKPRKTRNEENEQDGDLEGPVIDESVLS
  TKELLGLQQAEERLKRDCIDRLKRRPRNYPTAKYTCRLCDVLIESIAFAHKHIKEKRHKKNIKEKQEEEL
  LTTLPPPTPSQINAVGIAIDKVVQEFGLHNENLEQRLEIKRIMENVFQHKLPDCSLRLYGSSCSRLGFKN
  SDVNIDIQFPAIMSQPDVLLLVQECLKNSDSFIDVDADFHARVPVVVCREKQSGLLCKVSAGNENACLTT
  KHLTALGKLEPKLVPLVIAFRYWAKLCSIDRPEEGGLPPYVFALMAIFFLQQRKEPLLPVYLGSWIEGFS
  LSKLGNFNLQDIEKDVVIWEHTDSAAGDTGITKEEAPRETPIKRGQVSLILDVKHQPSVPVGQLWVELLR
  FYALEFNLADLVISIRVKELVSRELKDWPKKRIAIEDPYSVKRNVARTLNSQPVFEYILHCLRTTYKYFA
  LPHKITKSSLLKPLNAITCISEHSKEVINHHPDVQTKDDKLKNSVLAQGPGATSSAANTCKVQPLTLKET
  AESFGSPPKEEMGNEHISVHPENSDCIQADVNSDDYKGDKVYHPETGRKNEKEKVGRKGKHLLTVDQKRG
  EHVVCGSTRNNESESTLDLEGFQNPTAKECEGLATLDNKADLDGESTEGTEELEDSLNHFTHSVQGQTSE
  MIPSDEEEEDDEEEEEEEEPRLTINQREDEDGMANEDELDNTYTGSGDEDALSEEDDELGEAAKYEDVKE
  CGKHVERALLVELNKISLKEENVCEEKNSPVDQSDFFYEFSKLIFTKGKSPTVVCSLCKREGHLKKDCPE
  DFKRIQLEPLPPLTPKFLNILDQVCIQCYKDFSPTIIEDQAREHIRQNLESFIRQDFPGTKLSLFGSSKN
  GFGFKQSDLDVCMTINGLETAEGLDCVRTIEELARVLRKHSGLRNILPITTAKVPIVKFFHLRSGLEVDI
  SLYNTLALHNTRLLSAYSAIDPRVKYLCYTMKVFTKMCDIGDASRGSLSSYAYTLMVLYFLQQRNPPVIP
  VLQEIYKGEKKPEIFVDGWNIYFFDQIDELPTYWSECGKNTESVGQLWLGLLRFYTEEFDFKEHVISIRR
  KSLLTTFKKQWTSKYIVIEDPFDLNHNLGAGLSRKMTNFIMKAFINGRRVFGIPVKGFPKDYPSKMEYFF
  DPDVLTEGELAPNDRCCRICGKIGHFMKDCPMRRKVRRRRDQEDALNQRYPENKEKRSKEDKEIHNKYTE
  REVSTKEDKPIQCTPQKAKPMRAAADLGREKILRPPVEKWKRQDDKDLREKRCFICGREGHIKKECPQFK
  GSSGSLSSKYMTQGKASAKRTQQES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024617
• ORF Size: 4485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_024617.2, NM_024617.3, NP_078893.2
• RefSeq Size: 5648
• RefSeq ORF: 4488
• Locus ID: 79670
• Domains: zf-CCHC, PAP_assoc
• MW: 171 kDa