TAOK2 (NM_016151) Human Tagged ORF Clone

CAT#: RC214297

TAOK2 (Myc-DDK-tagged)-Human TAO kinase 2 (TAOK2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY414156) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214297 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAOK2
• Synonyms: MAP3K17; PSK; PSK1; PSK1-BETA; TAO1; TAO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214297 representing NM_016151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGGACCCAGATGTGGCTGAGCTCTTCTTCAAGGATGACC
  CAGAAAAGCTCTTCTCTGACCTCCGGGAAATTGGCCATGGCAGCTTTGGAGCCGTATACTTTGCCCGGGA
  TGTCCGGAATAGTGAGGTGGTGGCCATCAAGAAGATGTCCTACAGTGGGAAGCAGTCCAATGAGAAATGG
  CAAGACATCATCAAGGAGGTGCGGTTCTTACAGAAGCTCCGGCATCCCAACACCATTCAGTACCGGGGCT
  GTTACCTGAGGGAGCACACGGCTTGGCTGGTAATGGAGTATTGCCTGGGCTCAGCTTCTGACCTTCTAGA
  AGTGCACAAGAAACCCCTTCAGGAGGTAGAGATCGCAGCTGTGACCCACGGGGCGCTTCAGGGCCTGGCA
  TATCTGCACTCCCACAACATGATCCATAGGGATGTGAAGGCTGGAAACATCCTGCTGTCAGAGCCAGGGT
  TAGTGAAGCTAGGGGACTTTGGTTCTGCGTCCATCATGGCACCTGCCAACTCCTTCGTGGGCACCCCATA
  CTGGATGGCACCCGAGGTGATCCTGGCCATGGATGAGGGGCAGTACGATGGCAAAGTGGACGTCTGGTCC
  TTGGGGATAACCTGCATCGAGCTGGCTGAACGGAAACCACCGCTCTTTAACATGAATGCGATGAGTGCCT
  TATACCACATTGCACAGAACGAATCCCCCGTGCTCCAGTCAGGACACTGGTCTGAGTACTTCCGGAATTT
  TGTCGACTCCTGTCTTCAGAAAATCCCTCAAGACAGACCAACCTCAGAGGTTCTCCTGAAGCACCGCTTT
  GTGCTCCGGGAGCGGCCACCCACAGTCATCATGGACCTGATCCAGAGGACCAAGGATGCCGTGCGGGAGC
  TGGACAACCTGCAGTACCGCAAGATGAAGAAGATCCTGTTCCAAGAGGCACCCAACGGCCCTGGTGCCGA
  GGCCCCAGAGGAGGAAGAGGAGGCCGAGCCCTACATGCACCGGGCCGGGACTCTGACCAGCCTCGAGAGT
  AGCCACTCAGTGCCCAGCATGTCCATCAGCGCCTCCAGCCAGAGCAGCTCCGTCAACAGCCTAGCAGATG
  CCTCAGACAACGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGGCCCTGAAGCCCG
  GGAGATGGCCATGATGCAGGAGGGGGAGCACACAGTCACCTCTCACAGCTCCATTATCCACCGGCTGCCG
  GGCTCTGACAACCTATATGATGACCCCTACCAGCCAGAGATAACCCCCAGCCCTCTCCAGCCGCCTGCAG
  CCCCAGCTCCCACTTCCACCACCTCTTCCGCCCGCCGCCGGGCCTACTGCCGTAACCGAGACCACTTTGC
  CACCATCCGAACCGCCTCCCTGGTCAGCCGTCAGATCCAGGAGCATGAGCAGGACTCTGCGCTGCGGGAG
  CAGCTGAGCGGCTATAAGCGGATGCGACGACAGCACCAGAAGCAGCTGCTGGCCCTGGAGTCACGGCTGA
  GGGGTGAACGGGAGGAGCACAGTGCACGGCTGCAGCGGGAGCTTGAGGCGCAGCGGGCTGGCTTTGGGGC
  AGAGGCAGAAAAGCTGGCCCGGCGGCACCAGGCCATAGGTGAGAAGGAGGCACGAGCTGCCCAGGCCGAG
  GAGCGGAAGTTCCAGCAGCACATCCTTGGGCAGCAGAAGAAGGAGCTGGCTGCCCTGCTGGAGGCACAGA
  AGCGGACCTACAAACTTCGCAAGGAACAGCTGAAGGAGGAGCTCCAGGAGAACCCCAGCACTCCCAAGCG
  GGAGAAGGCCGAGTGGCTGCTGCGGCAGAAGGAGCAGCTCCAGCAGTGCCAGGCGGAGGAGGAAGCAGGG
  CTGCTGCGGCGGCAGCGCCAGTACTTTGAGCTGCAGTGTCGCCAGTACAAGCGCAAGATGTTGCTGGCTC
  GGCACAGCCTGGACCAGGACCTGCTGCGGGAGGACCTGAACAAGAAGCAGACCCAGAAGGACTTGGAGTG
  TGCACTGCTGCTTCGGCAGCACGAGGCCACGCGGGAGCTGGAGCTGCGGCAGCTCCAGGCCGTGCAGCGC
  ACGCGGGCTGAGCTCACCCGCCTGCAGCACCAGACGGAGCTGGGCAACCAGCTGGAGTACAACAAGCGGC
  GTGAGCAAGAGTTGCGGCAGAAGCATGCGGCCCAGGTTCGCCAGCAGCCCAAGAGCCTCAAAGTACGTGC
  AGGCCAGCGCCCCCCGGGCCTTCCACTCCCCATTCCTGGGGCTCTGGGCCCACCCAACACAGGCACCCCT
  ATAGAACAGCAGCCCTGCTCACCTGGCCAGGAGGCAGTCCTGGACCAAAGAATGCTTGGCGAGGAGGAGG
  AAGCAGTTGGAGAGAGAAGGATTCTGGGAAAGGAAGGGGCCACTTTGGAGCCCAAGCAGCAGAGGATTCT
  GGGGGAAGAATCAGGAGCCCCTAGTCCCAGTCCACAAAAACATGGGAGCCTGGTTGATGAGGAAGTTTGG
  GGTCTGCCTGAGGAGATAGAGGAGCTTAGGGTGCCCTCCCTTGTACCCCAGGAGAGGAGCATTGTTGGCC
  AGGAGGAGGCTGGGACATGGAGCTTGTGGGGGAAGGAGGATGAGAGTCTTCTGGATGAGGAGTTTGAGCT
  TGGCTGGGTCCAGGGCCCAGCACTGACTCCCGTCCCTGAGGAGGAGGAAGAAGAGGAAGAGGGGGCTCCG
  ATTGGGACCCCTAGGGATCCTGGAGATGGTTGTCCTTCCCCCGACATCCCTCCTGAACCCCCTCCAACAC
  ACCTGAGGCCCTGCCCTGCCAGCCAGCTCCCTGGACTCCTGTCCCATGGCCTCCTGGCCGGCCTCTCCTT
  TGCAGTGGGGTCCTCCTCTGGCCTCCTGCCCCTCCTGCTGCTGCTGCTGCTTCCATTGCTGGCAGCCCAG
  GGTGGGGGTGGCCTGCAGGCAGCGCTGCTGGCCCTTGAGGTGGGGCTGGTGGGTCTGGGGGCCTCCTACC
  TGCTCCTTTGTACAGCCCTGCACCTGCCCTCCAGTCTTTTCCTACTCCTGGCCCAGGGTACCGCACTGGG
  GGCCGTCCTGGGCCTGAGCTGGCGCCGAGGCCTCATGGGTGTTCCCCTGGGCCTTGGAGCTGCCTGGCTC
  TTAGCTTGGCCAGGCCTAGCTCTACCTCTGGTGGCTATGGCAGCGGGGGGCAGATGGGTGCGGCAGCAGG
  GCCCCCGGGTGCGCCGGGGCATATCTCGACTCTGGTTGCGGGTTCTGCTGCGCCTGTCACCCATGGCCTT
  CCGGGCCCTGCAGGGCTGTGGGGCTGTGGGGGACCGGGGTCTGTTTGCACTGTACCCCAAAACCAACAAG
  GATGGCTTCCGCAGCCGCCTGCCCGTCCCTGGGCCCCGGCGGCGTAATCCCCGCACCACCCAACACCCAT
  TAGCTCTGTTGGCAAGGGTCTGGGTCCTGTGCAAGGGCTGGAACTGGCGTCTGGCACGGGCCAGCCAGGG
  TTTAGCATCCCACTTGCCCCCGTGGGCCATCCACACACTGGCCAGCTGGGGCCTGCTTCGGGGTGAACGG
  CCCACCCGAATCCCCCGGCTACTACCACGCAGCCAGCGCCAGCTAGGGCCCCCTGCCTCCCGCCAGCCAC
  TGCCAGGGACTCTAGCCGGGCGGAGGTCACGCACCCGCCAGTCCCGGGCCCTGCCCCCCTGGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214297 representing NM_016151
  Red=Cloning site Green=Tags(s)
  
  MPAGGRAGSLKDPDVAELFFKDDPEKLFSDLREIGHGSFGAVYFARDVRNSEVVAIKKMSYSGKQSNEKW
  QDIIKEVRFLQKLRHPNTIQYRGCYLREHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAVTHGALQGLA
  YLHSHNMIHRDVKAGNILLSEPGLVKLGDFGSASIMAPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWS
  LGITCIELAERKPPLFNMNAMSALYHIAQNESPVLQSGHWSEYFRNFVDSCLQKIPQDRPTSEVLLKHRF
  VLRERPPTVIMDLIQRTKDAVRELDNLQYRKMKKILFQEAPNGPGAEAPEEEEEAEPYMHRAGTLTSLES
  SHSVPSMSISASSQSSSVNSLADASDNEEEEEEEEEEEEEEEGPEAREMAMMQEGEHTVTSHSSIIHRLP
  GSDNLYDDPYQPEITPSPLQPPAAPAPTSTTSSARRRAYCRNRDHFATIRTASLVSRQIQEHEQDSALRE
  QLSGYKRMRRQHQKQLLALESRLRGEREEHSARLQRELEAQRAGFGAEAEKLARRHQAIGEKEARAAQAE
  ERKFQQHILGQQKKELAALLEAQKRTYKLRKEQLKEELQENPSTPKREKAEWLLRQKEQLQQCQAEEEAG
  LLRRQRQYFELQCRQYKRKMLLARHSLDQDLLREDLNKKQTQKDLECALLLRQHEATRELELRQLQAVQR
  TRAELTRLQHQTELGNQLEYNKRREQELRQKHAAQVRQQPKSLKVRAGQRPPGLPLPIPGALGPPNTGTP
  IEQQPCSPGQEAVLDQRMLGEEEEAVGERRILGKEGATLEPKQQRILGEESGAPSPSPQKHGSLVDEEVW
  GLPEEIEELRVPSLVPQERSIVGQEEAGTWSLWGKEDESLLDEEFELGWVQGPALTPVPEEEEEEEEGAP
  IGTPRDPGDGCPSPDIPPEPPPTHLRPCPASQLPGLLSHGLLAGLSFAVGSSSGLLPLLLLLLLPLLAAQ
  GGGGLQAALLALEVGLVGLGASYLLLCTALHLPSSLFLLLAQGTALGAVLGLSWRRGLMGVPLGLGAAWL
  LAWPGLALPLVAMAAGGRWVRQQGPRVRRGISRLWLRVLLRLSPMAFRALQGCGAVGDRGLFALYPKTNK
  DGFRSRLPVPGPRRRNPRTTQHPLALLARVWVLCKGWNWRLARASQGLASHLPPWAIHTLASWGLLRGER
  PTRIPRLLPRSQRQLGPPASRQPLPGTLAGRRSRTRQSRALPPWR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016151
• ORF Size: 3705 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016151.1, NM_016151.2, NM_016151.3, NP_057235.2
• RefSeq Size: 4920
• RefSeq ORF: 3708
• Locus ID: 9344
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: MAPK signaling pathway
• MW: 138.1 kDa
• Gene Summary: This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]