Aminomethyltransferase (AMT) (NM_000481) Human Tagged ORF Clone

CAT#: RC214343

AMT (Myc-DDK-tagged)-Human aminomethyltransferase (AMT), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY424691) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214343 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified AMT protein (Cat# TP314343). The protein was produced from HEK293T cells transfected with AMT cDNA clone (Cat# RC214343) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AMT
• Synonyms: GCE; GCST; GCVT; NKH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214343 representing NM_000481
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGGGCTGTAAGTGTGGTGGCCCGTCTGGGCTTTCGCCTGCAGGCATTCCCCCCGGCCTTGTGTC
  GTCCACTTAGTTGCGCACAGGAGGTGCTCCGCAGGACACCGCTCTATGACTTCCACCTGGCCCACGGCGG
  GAAAATGGTGGCGTTTGCGGGTTGGAGTCTGCCAGTGCAGTACCGGGACAGTCACACTGACTCGCACCTG
  CACACACGCCAGCACTGCTCGCTCTTTGACGTGTCTCATATGCTGCAGACCAAGATACTTGGTAGTGACC
  GGGTGAAGCTGATGGAGAGTCTAGTGGTTGGAGACATTGCAGAGCTAAGACCAAACCAGGGGACACTGTC
  GCTGTTTACCAACGAGGCTGGAGGCATCTTAGATGACTTGATTGTAACCAATACTTCTGAGGGCCACCTG
  TATGTGGTGTCCAACGCTGGCTGCTGGGAGAAAGATTTGGCCCTCATGCAGGACAAGGTCAGGGAGCTTC
  AGAACCAGGGCAGAGATGTGGGCCTGGAGGTGTTGGATAATGCCCTGCTAGCTCTGCAAGGCCCCACTGC
  AGCCCAGGTACTACAGGCCGGCGTGGCAGATGACCTGAGGAAACTGCCCTTCATGACCAGTGCTGTGATG
  GAGGTGTTTGGCGTGTCTGGCTGCCGCGTGACCCGCTGTGGCTACACAGGAGAGGATGGTGTGGAGATCT
  CGGTGCCGGTAGCGGGGGCAGTTCACCTGGCAACAGCTATTCTGAAAAACCCAGAGGTGAAGCTGGCAGG
  GCTGGCAGCCAGGGACAGCCTGCGCCTGGAGGCAGGCCTCTGCCTGTATGGGAATGACATTGATGAACAC
  ACTACACCTGTGGAGGGCAGCCTCAGTTGGACACTGGGGAAGCGCCGCCGAGCTGCTATGGACTTCCCTG
  GAGCCAAGGTCATTGTTCCCCAGCTGAAGGGCAGGGTGCAGCGGAGGCGTGTGGGGTTGATGTGTGAGGG
  GGCCCCCATGCGGGCACACAGTCCCATCCTGAACATGGAGGGTACCAAGATTGGTACTGTGACTAGTGGC
  TGCCCCTCCCCCTCTCTGAAGAAGAATGTGGCGATGGGTTATGTGCCCTGCGAGTACAGTCGTCCAGGGA
  CAATGCTGCTGGTAGAGGTGCGGCGGAAGCAGCAGATGGCTGTAGTCAGCAAGATGCCCTTTGTGCCCAC
  AAACTACTATACCCTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214343 representing NM_000481
  Red=Cloning site Green=Tags(s)
  
  MQRAVSVVARLGFRLQAFPPALCRPLSCAQEVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHTDSHL
  HTRQHCSLFDVSHMLQTKILGSDRVKLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHL
  YVVSNAGCWEKDLALMQDKVRELQNQGRDVGLEVLDNALLALQGPTAAQVLQAGVADDLRKLPFMTSAVM
  EVFGVSGCRVTRCGYTGEDGVEISVPVAGAVHLATAILKNPEVKLAGLAARDSLRLEAGLCLYGNDIDEH
  TTPVEGSLSWTLGKRRRAAMDFPGAKVIVPQLKGRVQRRRVGLMCEGAPMRAHSPILNMEGTKIGTVTSG
  CPSPSLKKNVAMGYVPCEYSRPGTMLLVEVRRKQQMAVVSKMPFVPTNYYTLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000481
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000481.1, NM_000481.2, NM_000481.3, NP_000472.2
• RefSeq Size: 2117 bp
• RefSeq ORF: 1212 bp
• Locus ID: 275
• Cytogenetics: 3p21.31
• Domains: GCV_T
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways, Nitrogen metabolism, One carbon pool by folate
• MW: 43.8 kDa
• Gene Summary: 'This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]'