TAOK2 (NM_004783) Human Tagged ORF Clone

CAT#: RC214344

TAOK2 (Myc-DDK-tagged)-Human TAO kinase 2 (TAOK2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401503) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214344 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TAOK2 protein (Cat# TP314344). The protein was produced from HEK293T cells transfected with TAOK2 cDNA clone (Cat# RC214344) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAOK2
• Synonyms: MAP3K17; PSK; PSK1; PSK1-BETA; TAO1; TAO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214344 representing NM_004783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGGACCCAGATGTGGCTGAGCTCTTCTTCAAGGATGACC
  CAGAAAAGCTCTTCTCTGACCTCCGGGAAATTGGCCATGGCAGCTTTGGAGCCGTATACTTTGCCCGGGA
  TGTCCGGAATAGTGAGGTGGTGGCCATCAAGAAGATGTCCTACAGTGGGAAGCAGTCCAATGAGAAATGG
  CAAGACATCATCAAGGAGGTGCGGTTCTTACAGAAGCTCCGGCATCCCAACACCATTCAGTACCGGGGCT
  GTTACCTGAGGGAGCACACGGCTTGGCTGGTAATGGAGTATTGCCTGGGCTCAGCTTCTGACCTTCTAGA
  AGTGCACAAGAAACCCCTTCAGGAGGTAGAGATCGCAGCTGTGACCCACGGGGCGCTTCAGGGCCTGGCA
  TATCTGCACTCCCACAACATGATCCATAGGGATGTGAAGGCTGGAAACATCCTGCTGTCAGAGCCAGGGT
  TAGTGAAGCTAGGGGACTTTGGTTCTGCGTCCATCATGGCACCTGCCAACTCCTTCGTGGGCACCCCATA
  CTGGATGGCACCCGAGGTGATCCTGGCCATGGATGAGGGGCAGTACGATGGCAAAGTGGACGTCTGGTCC
  TTGGGGATAACCTGCATCGAGCTGGCTGAACGGAAACCACCGCTCTTTAACATGAATGCGATGAGTGCCT
  TATACCACATTGCACAGAACGAATCCCCCGTGCTCCAGTCAGGACACTGGTCTGAGTACTTCCGGAATTT
  TGTCGACTCCTGTCTTCAGAAAATCCCTCAAGACAGACCAACCTCAGAGGTTCTCCTGAAGCACCGCTTT
  GTGCTCCGGGAGCGGCCACCCACAGTCATCATGGACCTGATCCAGAGGACCAAGGATGCCGTGCGGGAGC
  TGGACAACCTGCAGTACCGCAAGATGAAGAAGATCCTGTTCCAAGAGGCACCCAACGGCCCTGGTGCCGA
  GGCCCCAGAGGAGGAAGAGGAGGCCGAGCCCTACATGCACCGGGCCGGGACTCTGACCAGCCTCGAGAGT
  AGCCACTCAGTGCCCAGCATGTCCATCAGCGCCTCCAGCCAGAGCAGCTCCGTCAACAGCCTAGCAGATG
  CCTCAGACAACGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAAGGCCCTGAAGCCCG
  GGAGATGGCCATGATGCAGGAGGGGGAGCACACAGTCACCTCTCACAGCTCCATTATCCACCGGCTGCCG
  GGCTCTGACAACCTATATGATGACCCCTACCAGCCAGAGATAACCCCCAGCCCTCTCCAGCCGCCTGCAG
  CCCCAGCTCCCACTTCCACCACCTCTTCCGCCCGCCGCCGGGCCTACTGCCGTAACCGAGACCACTTTGC
  CACCATCCGAACCGCCTCCCTGGTCAGCCGTCAGATCCAGGAGCATGAGCAGGACTCTGCGCTGCGGGAG
  CAGCTGAGCGGCTATAAGCGGATGCGACGACAGCACCAGAAGCAGCTGCTGGCCCTGGAGTCACGGCTGA
  GGGGTGAACGGGAGGAGCACAGTGCACGGCTGCAGCGGGAGCTTGAGGCGCAGCGGGCTGGCTTTGGGGC
  AGAGGCAGAAAAGCTGGCCCGGCGGCACCAGGCCATAGGTGAGAAGGAGGCACGAGCTGCCCAGGCCGAG
  GAGCGGAAGTTCCAGCAGCACATCCTTGGGCAGCAGAAGAAGGAGCTGGCTGCCCTGCTGGAGGCACAGA
  AGCGGACCTACAAACTTCGCAAGGAACAGCTGAAGGAGGAGCTCCAGGAGAACCCCAGCACTCCCAAGCG
  GGAGAAGGCCGAGTGGCTGCTGCGGCAGAAGGAGCAGCTCCAGCAGTGCCAGGCGGAGGAGGAAGCAGGG
  CTGCTGCGGCGGCAGCGCCAGTACTTTGAGCTGCAGTGTCGCCAGTACAAGCGCAAGATGTTGCTGGCTC
  GGCACAGCCTGGACCAGGACCTGCTGCGGGAGGACCTGAACAAGAAGCAGACCCAGAAGGACTTGGAGTG
  TGCACTGCTGCTTCGGCAGCACGAGGCCACGCGGGAGCTGGAGCTGCGGCAGCTCCAGGCCGTGCAGCGC
  ACGCGGGCTGAGCTCACCCGCCTGCAGCACCAGACGGAGCTGGGCAACCAGCTGGAGTACAACAAGCGGC
  GTGAGCAAGAGTTGCGGCAGAAGCATGCGGCCCAGGTTCGCCAGCAGCCCAAGAGCCTCAAATCTAAGGA
  GCTGCAGATCAAGAAGCAGTTCCAGGAGACGTGTAAGATCCAGACTCGGCAGTACAAGGCTCTGCGAGCA
  CACTTGCTGGAGACCACGCCCAAAGCTCAGCACAAGAGCCTCCTTAAGCGGCTCAAGGAAGAGCAGACCC
  GCAAGCTGGCGATCTTGGCGGAGCAGTATGACCAGTCCATCTCAGAGATGCTCAGCTCACAGGCGCTGCG
  GCTTGATGAGACCCAGGAGGCAGAGTTCCAGGCCCTTCGGCAGCAGCTTCAACAGGAGCTGGAGCTGCTC
  AACGCTTACCAGAGCAAGATCAAGATCCGCACAGAGAGCCAGCACGAGAGGGAGCTGCGGGAGCTGGAGC
  AGAGGGTCGCGCTGCGGCGGGCACTGCTGGAGCAGCGGGTGGAAGAGGAGCTGCTGGCCCTGCAGACAGG
  ACGCTCCGAGCGAATCCGCAGTCTGCTTGAGCGGCAGGCCCGTGAGATCGAGGCCTTCGATGCGGAAAGC
  ATGAGGCTGGGCTTCTCCAGCATGGCTCTGGGGGGCATCCCGGCTGAAGCTGCTGCCCAGGGCTATCCTG
  CTCCACCCCCTGCCCCAGCCTGGCCCTCCCGTCCCGTTCCCCGTTCTGGGGCACACTGGAGCCATGGCCC
  TCCTCCACCAGGCATGCCCCCTCCAGCCTGGCGTCAGCCGTCTCTGCTGGCTCCCCCAGGCCCCCCAAAC
  TGGCTGGGGCCCCCCACACAAAGTGGGACACCCCGTGGCGGAGCCCTGCTGCTGCTAAGAAACAGCCCCC
  AGCCCCTGCGGCGGGCAGCCTCGGGGGGCAGTGGCAGTGAGAATGTGGGCCCCCCTGCTGCCGCGGTGCC
  CGGGCCCCTGAGCCGCAGCACCAGTGTCGCTTCCCACATCCTCAATGGTTCTTCCCACTTCTATTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214344 representing NM_004783
  Red=Cloning site Green=Tags(s)
  
  MPAGGRAGSLKDPDVAELFFKDDPEKLFSDLREIGHGSFGAVYFARDVRNSEVVAIKKMSYSGKQSNEKW
  QDIIKEVRFLQKLRHPNTIQYRGCYLREHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAVTHGALQGLA
  YLHSHNMIHRDVKAGNILLSEPGLVKLGDFGSASIMAPANSFVGTPYWMAPEVILAMDEGQYDGKVDVWS
  LGITCIELAERKPPLFNMNAMSALYHIAQNESPVLQSGHWSEYFRNFVDSCLQKIPQDRPTSEVLLKHRF
  VLRERPPTVIMDLIQRTKDAVRELDNLQYRKMKKILFQEAPNGPGAEAPEEEEEAEPYMHRAGTLTSLES
  SHSVPSMSISASSQSSSVNSLADASDNEEEEEEEEEEEEEEEGPEAREMAMMQEGEHTVTSHSSIIHRLP
  GSDNLYDDPYQPEITPSPLQPPAAPAPTSTTSSARRRAYCRNRDHFATIRTASLVSRQIQEHEQDSALRE
  QLSGYKRMRRQHQKQLLALESRLRGEREEHSARLQRELEAQRAGFGAEAEKLARRHQAIGEKEARAAQAE
  ERKFQQHILGQQKKELAALLEAQKRTYKLRKEQLKEELQENPSTPKREKAEWLLRQKEQLQQCQAEEEAG
  LLRRQRQYFELQCRQYKRKMLLARHSLDQDLLREDLNKKQTQKDLECALLLRQHEATRELELRQLQAVQR
  TRAELTRLQHQTELGNQLEYNKRREQELRQKHAAQVRQQPKSLKSKELQIKKQFQETCKIQTRQYKALRA
  HLLETTPKAQHKSLLKRLKEEQTRKLAILAEQYDQSISEMLSSQALRLDETQEAEFQALRQQLQQELELL
  NAYQSKIKIRTESQHERELRELEQRVALRRALLEQRVEEELLALQTGRSERIRSLLERQAREIEAFDAES
  MRLGFSSMALGGIPAEAAAQGYPAPPPAPAWPSRPVPRSGAHWSHGPPPPGMPPPAWRQPSLLAPPGPPN
  WLGPPTQSGTPRGGALLLLRNSPQPLRRAASGGSGSENVGPPAAAVPGPLSRSTSVASHILNGSSHFYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004783
• ORF Size: 3147 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004783.1, NM_004783.2, NM_004783.3, NP_004774.1
• RefSeq Size: 4625 bp
• RefSeq ORF: 3150 bp
• Locus ID: 9344
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: MAPK signaling pathway
• MW: 119.1 kDa
• Gene Summary: This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]