GBP6 (NM_198460) Human Tagged ORF Clone

CAT#: RC214371

GBP6 (Myc-DDK-tagged)-Human guanylate binding protein family, member 6 (GBP6)

Product Images

Western blot validation of overexpression lysate (Cat# LY404942) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214371 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified GBP6 protein (Cat# TP314371). The protein was produced from HEK293T cells transfected with GBP6 cDNA clone (Cat# RC214371) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GBP6
• Synonyms: DKFZp686G0786; FLJ39135
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214371 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATCTGGACCCAAAATGTTGGCCCCCGTTTGCCTGGTGGAAAATAACAATGAGCAGCTATTGGTGA
  ACCAGCAAGCTATACAGATTCTTGAAAAGATTTCTCAGCCAGTGGTGGTGGTGGCCATTGTAGGACTGTA
  CCGTACAGGGAAATCCTACTTGATGAACCATCTGGCAGGACAGAATCATGGCTTCCCTCTGGGCTCCACG
  GTGCAGTCTGAAACCAAGGGCATCTGGATGTGGTGCGTGCCCCACCCATCCAAGCCAAACCACACCCTGG
  TCCTTCTGGACACCGAAGGTCTGGGCGATGTGGAAAAGGGTGACCCTAAGAATGACTCCTGGATCTTTGC
  CCTGGCTGTGCTCCTGTGCAGCACCTTTGTCTACAACAGCATGAGCACCATCAACCACCAGGCCCTGGAG
  CAGCTGCATTATGTGACGGAGCTCACAGAACTAATTAAGGCAAAGTCCTCCCCAAGGCCTGATGGAGTAG
  AAGATTCCACAGAGTTTGTGAGTTTCTTCCCAGACTTTCTTTGGACAGTACGGGATTTCACTCTGGAGCT
  GAAGTTGAACGGTCACCCTATCACAGAAGATGAATACCTGGAGAATGCCTTGAAGCTGATTCAAGGCAAT
  AATCCCAGAGTTCAAACATCCAATTTTCCCAGGGAGTGCATCAGGCGTTTCTTTCCAAAACGGAAGTGTT
  TCGTCTTTGACCGGCCAACAAATGACAAAGACCTTCTAGCCAATATTGAGAAGGTGTCAGAAAAGCAACT
  GGATCCCAAATTCCAGGAACAAACAAACATTTTCTGTTCTTACATCTTCACTCATGCAAGAACCAAGACC
  CTCAGGGAGGGAATCACAGTCACTGGGAATCGTCTGGGAACTCTGGCAGTGACTTATGTAGAGGCCATCA
  ACAGTGGAGCAGTGCCTTGTCTGGAGAATGCAGTGATAACTCTGGCCCAGCGTGAGAACTCAGCGGCCGT
  GCAGAGGGCATCTGACTACTACAGCCAGCAGATGGCCCAGCGAGTGAAGTTCCCCACAGACACGCTCCAG
  GAGCTGCTGGACGTGCATGCGGCCTGTGAGAGGGAAGCCATTGCAATCTTCATGGAGCACTCCTTCAAGG
  ATGAAAATCAGGAATTCCAGAAGAAGTTCATGGAAACCACAATGAATAAGAAGGGGGATTTCTTGCTGCA
  GAATGAAGAGTCATCTGTTCAATACTGCCAGGCTAAACTCAATGAGCTCTCAAAGGGACTAATGGAAAGT
  ATCTCAGCAGGAAGTTTCTCTGTTCCTGGAGGGCACAAGCTCTACATGGAAACAAAGGAAAGGATTGAAC
  AGGACTATTGGCAAGTTCCCAGGAAAGGAGTAAAGGCAAAAGAGGTCTTCCAGAGGTTCCTGGAGTCACA
  GATGGTGATAGAGGAATCCATCTTGCAGTCAGATAAAGCCCTCACTGATAGAGAGAAGGCAGTAGCAGTG
  GATCGGGCCAAGAAGGAGGCAGCTGAGAAGGAACAGGAACTTTTAAAACAGAAATTACAGGAGCAGCAGC
  AACAGATGGAGGCTCAAGTTAAGAGTCGCAAGGAAAACATAGCCCAACTGAAGGAGAAGCTGCAGATGGA
  GAGAGAACACCTACTGAGAGAGCAGATTATGATGTTGGAGCACACGCAGAAGGTCCAAAATGATTGGCTT
  CATGAAGGATTTAAGAAGAAGTATGAGGAGATGAATGCAGAGATAAGTCAATTTAAACGTATGATTGATA
  CTACAAAAAATGATGATACTCCCTGGATTGCACGAACCTTGGACAACCTTGCCGATGAGCTAACTGCAAT
  ATTGTCTGCTCCTGCTAAATTAATTGGTCATGGTGTCAAAGGTGTGAGCTCACTCTTTAAAAAGCATAAG
  CTCCCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214371 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESGPKMLAPVCLVENNNEQLLVNQQAIQILEKISQPVVVVAIVGLYRTGKSYLMNHLAGQNHGFPLGST
  VQSETKGIWMWCVPHPSKPNHTLVLLDTEGLGDVEKGDPKNDSWIFALAVLLCSTFVYNSMSTINHQALE
  QLHYVTELTELIKAKSSPRPDGVEDSTEFVSFFPDFLWTVRDFTLELKLNGHPITEDEYLENALKLIQGN
  NPRVQTSNFPRECIRRFFPKRKCFVFDRPTNDKDLLANIEKVSEKQLDPKFQEQTNIFCSYIFTHARTKT
  LREGITVTGNRLGTLAVTYVEAINSGAVPCLENAVITLAQRENSAAVQRASDYYSQQMAQRVKFPTDTLQ
  ELLDVHAACEREAIAIFMEHSFKDENQEFQKKFMETTMNKKGDFLLQNEESSVQYCQAKLNELSKGLMES
  ISAGSFSVPGGHKLYMETKERIEQDYWQVPRKGVKAKEVFQRFLESQMVIEESILQSDKALTDREKAVAV
  DRAKKEAAEKEQELLKQKLQEQQQQMEAQVKSRKENIAQLKEKLQMEREHLLREQIMMLEHTQKVQNDWL
  HEGFKKKYEEMNAEISQFKRMIDTTKNDDTPWIARTLDNLADELTAILSAPAKLIGHGVKGVSSLFKKHK
  LPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198460
• ORF Size: 1899 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198460.1, NM_198460.2, NP_940862.1, NP_940862.2
• RefSeq Size: 4867 bp
• RefSeq ORF: 1902 bp
• Locus ID: 163351
• MW: 72.4 kDa
• Gene Summary: Guanylate-binding proteins, such as GBP6, are induced by interferon and hydrolyze GTP to both GDP and GMP (Olszewski et al., 2006 [PubMed 16689661]). [supplied by OMIM, Dec 2008]