PHF21A (NM_016621) Human Tagged ORF Clone

CAT#: RC214381

PHF21A (Myc-DDK-tagged)-Human PHD finger protein 21A (PHF21A), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHF21A
• Synonyms: BHC80; BM-006
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214381 representing NM_016621
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGCAGACTCTACAGGAGGCTCTTAAAGTGGAAATTCAGGTTCACCAGAAACTGGTTGCTCAAA
  TGAAGCAGGATCCACAGAATGCTGACTTAAAGAAACAGCTTCATGAACTCCAAGCCAAAATCACAGCTTT
  GAGTGAGAAACAGAAAAGAGTAGTTGAACAGCTACGGAAGAACCTGATAGTAAAGCAAGAACAACCGGAC
  AAGTTCCAAATACAGCCATTGCCACAATCTGAAAACAAACTACAAACAGCACAGCAGCAACCACTACAGC
  AACTACAACAACAGCAGCAGTACCACCACCACCACGCCCAGCAGTCAGCTGCAGCCTCTCCCAACCTGAC
  TGCTTCACAGAAGACTGTAACTACAGCTTCTATGATTACCACAAAGACACTACCTCTCGTCTTGAAAGCA
  GCAACTGCGACCATGCCTGCCTCTGTGGTGGGCCAGAGACCTACCATTGCTATGGTGACCGCCATCAACA
  GTCAGAAGGCTGTGCTCAGCACTGATGTGCAGAACACACCAGTCAACCTCCAGACGTCTAGTAAGGTCAC
  TGGGCCTGGGGCAGAGGCTGTCCAAATTGTGGCAAAAAACACAGTCACTCTGCAGGTTCAGGCAACACCT
  CCTCAGCCCATCAAAGTACCACAGTTTATCCCCCCTCCTAGACTCACTCCACGTCCAAACTTTCTTCCAC
  AGGTTCGACCCAAGCCTGTGGCCCAGAATAACATTCCTATTGCCCCAGCACCACCTCCCATGCTCGCAGC
  TCCTCAGCTTATCCAGAGGCCCGTCATGCTGACCAAGTTCACCCCCACAACCCTTCCCACATCCCAGAAT
  TCCATCCACCCCGTCCGTGTCGTCAATGGGCAGACTGCAACCATAGCCAAAACGTTCCCCATGGCCCAGC
  TCACCAGCATTGTGATAGCTACTCCAGGGACCAGACTCGCTGGACCTCAAACTGTACAGCTTAGCAAGCC
  AAGTCTTGAAAAACAGACAGTTAAATCTCACACAGAAACAGATGAGAAACAAACAGAGAGCCGCACCATC
  ACCCCACCTGCTGCACCCAAACCAAAACGGGAGGAGAACCCTCAGAAACTTGCCTTCATGGTGTCTCTAG
  GGTTGGTAACACATGACCATCTAGAAGAAATCCAAAGCAAGAGGCAAGAGCGAAAAAGAAGAACAACAGC
  AAATCCGGTCTACAGTGGAGCAGTCTTTGAGCCAGAGCGTAAGAAGAGTGCAGTGACATACCTAAACAGC
  ACAATGCACCCTGGGACCCGGAAGAGAGCCAATGAGGAACACTGGCCAAAGGGTGATATTCATGAGGATT
  TTTGCAGCGTTTGCAGAAAAAGTGGCCAGTTACTGATGTGCGACACATGTTCCCGTGTATATCATTTGGA
  CTGCTTAGACCCCCCTCTGAAAACAATTCCCAAGGGCATGTGGATCTGTCCCAGATGTCAGGACCAGATG
  CTGAAGAAGGAAGAAGCAATTCCATGGCCTGGAACTTTAGCAATTGTTCATTCCTATATTGCCTACAAAG
  CAGCAAAAGAAGAAGAGAAACAGAAGTTACTTAAATGGAGTTCAGATTTAAAACAAGAACGAGAACAACT
  AGAGCAAAAGGTGAAACAGCTCAGCAATTCCATAAGTAAATGCATGGAAATGAAGAACACCATCCTGGCC
  CGGCAGAAGGAGATGCACAGCTCCCTGGAGAAGGTAAAACAGCTGATTCGCCTCATCCACGGCATCGACC
  TCTCCAAACCTGTAGACTCTGAGGCCACTGTGGGGGCCATCTCCAATGGCCCGGACTGCACCCCCCCTGC
  CAATGCCGCCACCTCCACGCCGGCCCCTTCCCCCTCCTCCCAGAGCTGCACAGCGAACTGTAACCAGGGG
  GAAGAGACTAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214381 representing NM_016621
  Red=Cloning site Green=Tags(s)
  
  MELQTLQEALKVEIQVHQKLVAQMKQDPQNADLKKQLHELQAKITALSEKQKRVVEQLRKNLIVKQEQPD
  KFQIQPLPQSENKLQTAQQQPLQQLQQQQQYHHHHAQQSAAASPNLTASQKTVTTASMITTKTLPLVLKA
  ATATMPASVVGQRPTIAMVTAINSQKAVLSTDVQNTPVNLQTSSKVTGPGAEAVQIVAKNTVTLQVQATP
  PQPIKVPQFIPPPRLTPRPNFLPQVRPKPVAQNNIPIAPAPPPMLAAPQLIQRPVMLTKFTPTTLPTSQN
  SIHPVRVVNGQTATIAKTFPMAQLTSIVIATPGTRLAGPQTVQLSKPSLEKQTVKSHTETDEKQTESRTI
  TPPAAPKPKREENPQKLAFMVSLGLVTHDHLEEIQSKRQERKRRTTANPVYSGAVFEPERKKSAVTYLNS
  TMHPGTRKRANEEHWPKGDIHEDFCSVCRKSGQLLMCDTCSRVYHLDCLDPPLKTIPKGMWICPRCQDQM
  LKKEEAIPWPGTLAIVHSYIAYKAAKEEEKQKLLKWSSDLKQEREQLEQKVKQLSNSISKCMEMKNTILA
  RQKEMHSSLEKVKQLIRLIHGIDLSKPVDSEATVGAISNGPDCTPPANAATSTPAPSPSSQSCTANCNQG
  EETK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016621
• ORF Size: 1902 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016621.2, NM_016621.3, NP_057705.3
• RefSeq Size: 7194
• RefSeq ORF: 1905
• Locus ID: 51317
• Domains: PHD
• Protein Families: Druggable Genome, Transcription Factors
• MW: 70 kDa
• Gene Summary: The PHF21A gene encodes BHC80, a component of a BRAF35 (MIM 605535)/histone deacetylase (HDAC; see MIM 601241) complex (BHC) that mediates repression of neuron-specific genes through the cis-regulatory element known as repressor element-1 (RE1) or neural restrictive silencer (NRS) (Hakimi et al., 2002 [PubMed 12032298]). [supplied by OMIM, Nov 2010]