DAB2IP (NM_032552) Human Tagged ORF Clone

CAT#: RC214413

DAB2IP (Myc-DDK-tagged)-Human DAB2 interacting protein (DAB2IP), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410041) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214413 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DAB2IP protein (Cat# TP314413). The protein was produced from HEK293T cells transfected with DAB2IP cDNA clone (Cat# RC214413) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAB2IP
• Synonyms: AF9Q34; AIP-1; AIP1; DIP1/2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214413 representing NM_032552
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGACTCCCTTCTGGACCAAGACGACTCCTACGAGTCGCCTCAAGAAAGGCCGGGCTCTCGGC
  GCAGCCTGCCTGGCAGCCTTTCCGAGAAGAGCCCCAGCATGGAGCCCTCGGCCGCCACGCCGTTCCGGGT
  CACGGGCTTCCTCAGCCGCCGCCTCAAGGGCTCCATCAAGCGCACCAAGAGCCAGCCCAAGCTGGACCGC
  AACCACAGCTTCCGCCACATCCTGCCGGGGTTCCGGAGCGCCGCCGCCGCCGCCGCGGACAATGAGAGGT
  CCCATCTGATGCCGAGGCTGAAGGAGTCTCGCTCCCACGAGTCCCTGCTCAGCCCCAGCAGTGCGGTGGA
  GGCGCTGGACCTCAGCATGGAGGAAGAGGTGGTCATCAAGCCCGTGCACAGCAGCATCCTTGGCCAGGAC
  TACTGCTTCGAGGTGACGACGTCATCAGGAAGCAAGTGCTTTTCCTGCCGGTCTGCAGCTGAGCGGGATA
  AGTGGATGGAGAACCTCCGGCGAGCGGTGCATCCCAACAAGGACAACAGCCGGCGTGTGGAGCACATCCT
  GAAGCTGTGGGTGATCGAGGCCAAGGACCTGCCAGCCAAGAAGAAGTACCTGTGCGAGCTGTGCCTGGAC
  GATGTGCTCTATGCCCGCACCACGGGCAAGCTCAAGACGGACAATGTTTTCTGGGGCGAGCACTTCGAGT
  TCCACAACTTGCCGCCTCTGCGCACGGTCACTGTCCACCTGTACCGGGAGACCGACAAGAAGAAGAAGAA
  GGAGCGCAACAGTTACCTGGGCCTGGTGAGCCTACCTGCTGCCTCGGTGGCCGGGCGGCAGTTCGTGGAG
  AAGTGGTACCCGGTGGTGACGCCCAACCCCAAGGGCGGCAAGGGCCCTGGACCCATGATCCGCATCAAGG
  CGCGCTACCAAACCATCACCATCCTGCCCATGGAGATGTACAAAGAGTTCGCTGAGCACATCACCAACCA
  CTACCTGGGGCTGTGTGCAGCCCTCGAGCCCATCCTCAGTGCCAAGACCAAGGAGGAGATGGCATCTGCC
  CTGGTGCACATCCTGCAGAGCACGGGCAAGGTGAAGGACTTCCTGACAGACCTGATGATGTCAGAGGTGG
  ACCGCTGCGGGGACAACGAGCACCTCATCTTCCGGGAGAACACACTGGCCACCAAGGCCATTGAGGAGTA
  CCTCAAGCTAGTGGGCCAGAAGTACCTGCAGGACGCCCTAGGTGAGTTCATCAAAGCGCTGTATGAGTCA
  GATGAGAACTGCGAAGTGGATCCCAGCAAGTGCTCGGCCGCTGACCTCCCAGAGCACCAGGGCAACCTCA
  AGATGTGCTGCGAGCTGGCCTTCTGCAAGATCATCAACTCCTACTGTGTCTTCCCACGGGAGTTGAAAGA
  GGTGTTTGCCTCGTGGAGGCAGGAGTGCAGCAGTCGCGGCCGCCCGGACATCAGTGAGCGGCTCATCAGC
  GCCTCCCTCTTCCTGCGCTTCCTCTGCCCAGCCATCATGTCGCCCTCACTCTTCAACCTGCTGCAGGAGT
  ACCCTGATGACCGCACTGCCCGCACCCTCACCCTCATCGCCAAGGTCACCCAGAACCTGGCCAACTTTGC
  CAAATTTGGCAGCAAGGAGGAATACATGTCCTTCATGAACCAGTTCCTAGAGCATGAGTGGACCAACATG
  CAGCGCTTCCTGCTGGAGATCTCCAACCCCGAGACCCTCTCCAATACAGCCGGCTTCGAGGGCTACATCG
  ACCTGGGCCGCGAGCTCTCCAGCCTGCACTCACTGCTCTGGGAGGCCGTCAGCCAGCTGGAGCAGAGCAT
  AGTATCCAAACTGGGACCCCTGCCTCGGATCCTGAGGGACGTCCACACAGCACTGAGCACCCCAGGTAGC
  GGGCAGCTCCCAGGGACCAATGACCTGGCCTCCACACCGGGCTCTGGCAGCAGCAGCATCTCAGCTGGGC
  TGCAGAAGATGGTGATTGAGAACGATCTTTCCGGTCTGATAGATTTCACCCGGTTACCGTCTCCAACCCC
  CGAAAACAAGGACTTGTTTTTTGTCACAAGGTCCTCCGGGGTCCAGCCCTCACCTGCCCGCAGCTCGAGT
  TACTCGGAAGCCAACGAGCCTGATCTTCAGATGGCCAACGGTGGCAAGAGCCTCTCCATGGTGGACCTCC
  AGGACGCCCGCACGCTGGATGGGGAGGCAGGCTCCCCGGCGGGCCCCGACGTCCTCCCCACAGATGGGCA
  GGCCGCTGCAGCTCAGCTGGTGGCCGGGTGGCCGGCCCGGGCAACCCCAGTGAACCTGGCAGGGCTGGCC
  ACGGTGCGGCGGGCAGGCCAGACACCAACCACACCAGGCACCTCCGAGGGCGCGCCAGGCCGGCCCCAGC
  TGTTGGCACCGCTCTCCTTCCAGAACCCTGTGTACCAGATGGCGGCTGGCCTGCCGCTGTCACCCCGTGG
  CCTTGGCGACTCAGGCTCTGAGGGCCACAGCTCCCTGAGCTCACACAGCAACAGCGAGGAGTTGGCGGCT
  GCTGCCAAGCTGGGAAGTTTCAGCACTGCCGCGGAGGAGCTGGCTCGGCGGCCCGGTGAGCTGGCACGGC
  GACAGATGTCACTGACTGAAAAAGGCGGGCAGCCCACGGTGCCACGGCAGAACAGTGCTGGCCCCCAGAG
  GAGGATCGACCAGCCTCCGCCCCCACCCCCGCCGCCACCTCCTGCCCCCCGCGGCCGGACGCCCCCCAAC
  CTGCTGAGCACCCTGCAGTACCCAAGACCCTCAAGCGGAACCCTGGCGTCGGCCTCACCTGATTGGGTGG
  GCCCCAGTACCCGCCTGAGGCAGCAGTCCTCTTCCTCCAAGGGGGACAGCCCAGAACTGAAGCCACGGGC
  AGTGCACAAGCAGGGCCCTTCACCTGTGAGCCCCAATGCCCTGGACCGCACAGCCGCTTGGCTCTTGACC
  ATGAACGCGCAGTTGTTAGAAGACGAGGGCCTGGGCCCAGACCCCCCCCACAGGGATAGGCTAAGGAGTA
  AGGACGAGCTCAGCCAAGCAGAAAAGGACCTGGCGGTGCTGCAGGACAAGCTGCGAATCTCCACCAAGAA
  GCTGGAGGAGTATGAGACCCTGTTCAAGTGCCAGGAGGAGACGACGCAGAAGCTGGTGCTGGAGTACCAG
  GCACGGCTGGAGGAGGGCGAGGAGCGGCTGCGGCGGCAGCAGGAGGACAAGGACATCCAGATGAAGGGCA
  TCATCAGCAGGTTGATGTCCGTGGAGGAAGAACTGAAGAAGGACCACGCAGAGATGCAAGCGGCTGTGGA
  CTCCAAACAGAAGATCATTGATGCCCAGGAGAAGCGCATTGCCTCGTTGGATGCCGCCAATGCCCGCCTC
  ATGAGTGCCCTGACCCAGCTGAAAGAGAGTATGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214413 representing NM_032552
  Red=Cloning site Green=Tags(s)
  
  MEPDSLLDQDDSYESPQERPGSRRSLPGSLSEKSPSMEPSAATPFRVTGFLSRRLKGSIKRTKSQPKLDR
  NHSFRHILPGFRSAAAAAADNERSHLMPRLKESRSHESLLSPSSAVEALDLSMEEEVVIKPVHSSILGQD
  YCFEVTTSSGSKCFSCRSAAERDKWMENLRRAVHPNKDNSRRVEHILKLWVIEAKDLPAKKKYLCELCLD
  DVLYARTTGKLKTDNVFWGEHFEFHNLPPLRTVTVHLYRETDKKKKKERNSYLGLVSLPAASVAGRQFVE
  KWYPVVTPNPKGGKGPGPMIRIKARYQTITILPMEMYKEFAEHITNHYLGLCAALEPILSAKTKEEMASA
  LVHILQSTGKVKDFLTDLMMSEVDRCGDNEHLIFRENTLATKAIEEYLKLVGQKYLQDALGEFIKALYES
  DENCEVDPSKCSAADLPEHQGNLKMCCELAFCKIINSYCVFPRELKEVFASWRQECSSRGRPDISERLIS
  ASLFLRFLCPAIMSPSLFNLLQEYPDDRTARTLTLIAKVTQNLANFAKFGSKEEYMSFMNQFLEHEWTNM
  QRFLLEISNPETLSNTAGFEGYIDLGRELSSLHSLLWEAVSQLEQSIVSKLGPLPRILRDVHTALSTPGS
  GQLPGTNDLASTPGSGSSSISAGLQKMVIENDLSGLIDFTRLPSPTPENKDLFFVTRSSGVQPSPARSSS
  YSEANEPDLQMANGGKSLSMVDLQDARTLDGEAGSPAGPDVLPTDGQAAAAQLVAGWPARATPVNLAGLA
  TVRRAGQTPTTPGTSEGAPGRPQLLAPLSFQNPVYQMAAGLPLSPRGLGDSGSEGHSSLSSHSNSEELAA
  AAKLGSFSTAAEELARRPGELARRQMSLTEKGGQPTVPRQNSAGPQRRIDQPPPPPPPPPPAPRGRTPPN
  LLSTLQYPRPSSGTLASASPDWVGPSTRLRQQSSSSKGDSPELKPRAVHKQGPSPVSPNALDRTAAWLLT
  MNAQLLEDEGLGPDPPHRDRLRSKDELSQAEKDLAVLQDKLRISTKKLEEYETLFKCQEETTQKLVLEYQ
  ARLEEGEERLRRQQEDKDIQMKGIISRLMSVEEELKKDHAEMQAAVDSKQKIIDAQEKRIASLDAANARL
  MSALTQLKESMH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032552
• ORF Size: 3396 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032552.2, NM_032552.3, NP_115941.2
• RefSeq Size: 5472
• RefSeq ORF: 3399
• Locus ID: 153090
• MW: 124.9 kDa
• Gene Summary: DAB2IP is a Ras (MIM 190020) GTPase-activating protein (GAP) that acts as a tumor suppressor. The DAB2IP gene is inactivated by methylation in prostate and breast cancers (Yano et al., 2005 [PubMed 15386433]). [supplied by OMIM, May 2010]