ENTPD4 (NM_004901) Human Tagged ORF Clone

CAT#: RC214422

ENTPD4 (Myc-DDK-tagged)-Human ectonucleoside triphosphate diphosphohydrolase 4 (ENTPD4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417671) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214422 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ENTPD4 protein (Cat# TP314422). The protein was produced from HEK293T cells transfected with ENTPD4 cDNA clone (Cat# RC214422) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ENTPD4
• Synonyms: LALP70; LAP70; LYSAL1; NTPDase-4; UDPase
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214422 representing NM_004901
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGGATTGGCATCTCCTGTCTTTTTCCTGCTTCTTGGCATTTTAGCATATCTCCAGTAGGGTGTC
  CTCGAATTCTGAATACCAATTTACGCCAAATTATGGTCATTAGTGTCCTGGCTGCTGCTGTTTCACTTTT
  ATATTTTTCTGTTGTCATAATCCGAAATAAGTATGGGCGACTAACCAGAGACAAGAAATTTCAAAGGTAC
  CTGGCACGAGTTACCGACATTGAAGCTACAGACACCAATAACCCCAATGTGAACTATGGGATCGTGGTGG
  ACTGTGGTAGCAGTGGGTCTCGAGTATTTGTTTACTGCTGGCCAAGGCATAATGGCAATCCACATGATCT
  GTTGGATATCAGGCAAATGAGGGATAAAAACCGAAAGCCAGTGGTCATGAAAATAAAACCGGGCATTTCA
  GAATTTGCTACCTCTCCAGAGAAAGTCAGTGATTACATTTCTCCACTTTTGAACTTTGCTGCAGAGCATG
  TGCCACGGGCAAAACACAAAGAGACACCTCTCTACATTCTCTGCACGGCTGGAATGAGAATCCTCCCCGA
  AAGCCAGCAGAAAGCTATTCTGGAAGACCTTCTGACCGATATCCCCGTGCACTTTGACTTTCTGTTTTCT
  GACTCTCATGCAGAAGTAATTTCTGGGAAACAGGAAGGTGTGTATGCTTGGATTGGCATTAATTTTGTCC
  TTGGACGATTTGAGCATATTGAAGATGATGATGAGGCCGTTGTGGAAGTTAACATTCCTGGAAGTGAAAG
  CAGCGAAGCCATTGTCCGTAAAAGGACAGCGGGCATTCTCGACATGGGCGGCGTGTCGACTCAGATAGCG
  TACGAAGTCCCCAAAACTGTAAGCTTTGCGTCCTCACAGCAGGAAGAAGTAGCTAAAAACTTGTTAGCTG
  AATTTAACTTGGGATGTGATGTTCACCAAACTGAGCATGTGTATCGAGTCTATGTGGCCACGTTTCTTGG
  GTTTGGTGGCAATGCTGCTCGACAGAGATACGAAGACAGAATATTTGCCAACACCATTCAAAAGAACAGG
  CTCCTGGGTAAACAGACTGGTCTGACTCCTGATATGCCGTACTTGGACCCCTGCCTACCCCTAGACATTA
  AAGATGAAATCCAGCAAAATGGACAAACCATATACCTACGAGGGACTGGAGACTTTGACCTGTGTCGAGA
  GACTATCCAGCCTTTCATGAATAAAACAAACGAGACCCAGACTTCCCTCAATGGGGTCTACCAGCCCCCA
  ATTCACTTCCAGAACAGTGAATTCTATGGCTTCTCCGAATTCTACTACTGCACCGAGGATGTGTTACGAA
  TGGGGGGAGACTACAATGCTGCTAAATTTACTAAAGCTGCAAAGGATTATTGTGCAACAAAGTGGTCCAT
  TTTGCGGGAACGCTTTGACCGAGGACTGTACGCCTCTCATGCTGACCTCCACAGGCTTAAGTATCAGTGC
  TTCAAATCGGCCTGGATGTTTGAGGTGTTTCATAGGGGCTTTTCGTTTCCTGTCAACTATAAAAGCTTAA
  AGACTGCCTTGCAAGTTTACGACAAGGAGGTTCAGTGGACCCTTGGAGCCATCCTCTACAGGACCCGCTT
  TCTACCGTTAAGAGACATCCAGCAGGAGGCCTTCCGAGCCAGTCACACCCACTGGCGGGGCGTTTCCTTT
  GTCTACAACCACTACCTGTTCTCTGGCTGCTTCCTGGTGGTGCTGCTGGCCATCCTGCTGTACCTGCTGC
  GGCTGCGGCGCATCCACAGGCGCACTCCCCGGAGCAGCTCGGCCGCCGCCCTCTGGATGGAGGAGGGCCT
  TCCCGCCCAGAATGCCCCGGGGACCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214422 representing NM_004901
  Red=Cloning site Green=Tags(s)
  
  MGRIGISCLFPASWHFSISPVGCPRILNTNLRQIMVISVLAAAVSLLYFSVVIIRNKYGRLTRDKKFQRY
  LARVTDIEATDTNNPNVNYGIVVDCGSSGSRVFVYCWPRHNGNPHDLLDIRQMRDKNRKPVVMKIKPGIS
  EFATSPEKVSDYISPLLNFAAEHVPRAKHKETPLYILCTAGMRILPESQQKAILEDLLTDIPVHFDFLFS
  DSHAEVISGKQEGVYAWIGINFVLGRFEHIEDDDEAVVEVNIPGSESSEAIVRKRTAGILDMGGVSTQIA
  YEVPKTVSFASSQQEEVAKNLLAEFNLGCDVHQTEHVYRVYVATFLGFGGNAARQRYEDRIFANTIQKNR
  LLGKQTGLTPDMPYLDPCLPLDIKDEIQQNGQTIYLRGTGDFDLCRETIQPFMNKTNETQTSLNGVYQPP
  IHFQNSEFYGFSEFYYCTEDVLRMGGDYNAAKFTKAAKDYCATKWSILRERFDRGLYASHADLHRLKYQC
  FKSAWMFEVFHRGFSFPVNYKSLKTALQVYDKEVQWTLGAILYRTRFLPLRDIQQEAFRASHTHWRGVSF
  VYNHYLFSGCFLVVLLAILLYLLRLRRIHRRTPRSSSAAALWMEEGLPAQNAPGTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004901
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004901.1, NM_004901.2, NM_004901.3, NM_004901.4, NP_004892.1
• RefSeq Size: 2330 bp
• RefSeq ORF: 1851 bp
• Locus ID: 9583
• Domains: GDA1_CD39
• Protein Families: Transmembrane
• Protein Pathways: Lysosome, Purine metabolism, Pyrimidine metabolism
• MW: 70.1 kDa
• Gene Summary: This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. [provided by RefSeq, Sep 2011]