NSUN2 (NM_017755) Human Tagged ORF Clone
CAT#: RC214459
- TrueORF®
NSUN2 (Myc-DDK-tagged)-Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1
"NM_017755" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NSUN2 |
Synonyms | MISU; MRT5; SAKI; TRM4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214459 representing NM_017755
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGGCGGTCGCGGGGTCGGCGGCTCCAGCAACAGCAGCGGCCGGAGGACGCGGAGGATGGCGCCG AGGGTGGTGGAAAGCGCGGCGAGGCGGGCTGGGAAGGAGGCTACCCCGAGATCGTCAAGGAGAACAAGCT GTTCGAGCACTACTACCAGGAGCTCAAGATCGTGCCCGAGGGCGAGTGGGGCCAGTTCATGGACGCTCTC AGGGAGCCGCTCCCGGCCACTTTAAGAATTACTGGTTACAAAAGCCACGCAAAAGAGATTCTCCATTGCT TAAAGAACAAATATTTTAAGGAATTGGAGGACCTGGAGGTGGACGGTCAGAAAGTTGAAGTTCCACAGCC ACTGAGTTGGTATCCTGAAGAACTTGCCTGGCACACAAATTTAAGTCGAAAAATCTTGAGAAAATCGCCA CACTTGGAAAAGTTTCATCAGTTTCTAGTTAGTGAAACAGAATCTGGAAATATTAGTCGTCAAGAAGCTG TTAGCATGATCCCACCACTGCTCCTCAACGTGCGGCCTCATCATAAGATCTTAGATATGTGTGCAGCACC TGGCTCAAAGACCACACAGTTAATTGAAATGCTACATGCCGACATGAATGTCCCCTTTCCAGAGGGATTT GTTATTGCGAATGATGTGGACAACAAGCGCTGCTACCTGCTCGTCCATCAAGCCAAGAGGCTGAGCAGCC CCTGCATCATGGTGGTCAACCATGATGCCTCCAGCATACCCAGGCTCCAGATAGATGTGGACGGCAGGAA AGAGATCCTCTTCTATGATCGAATTTTATGTGATGTCCCTTGCAGTGGAGACGGCACTATGAGAAAAAAC ATTGATGTTTGGAAAAAGTGGACCACCTTAAATAGCTTGCAGCTACATGGCTTACAGCTGCGGATTGCAA CACGCGGGGCTGAACAGCTGGCTGAAGGTGGAAGGATGGTGTATTCCACGTGTTCACTAAACCCTATTGA GGATGAAGCAGTCATAGCATCTTTACTGGAAAAAAGTGAAGGTGCTTTGGAGCTTGCTGATGTGTCTAAT GAACTGCCAGGGCTGAAGTGGATGCCTGGAATCACACAGTGGAAGGTAATGACGAAAGATGGGCAGTGGT TTACAGACTGGGACGCTGTTCCTCACAGCAGACACACCCAGATCCGACCTACCATGTTCCCTCCGAAGGA CCCAGAAAAGCTGCAGGCCATGCACCTGGAGCGATGCCTTAGGATATTACCCCATCATCAGAATACTGGA GGGTTTTTTGTGGCAGTATTGGTGAAAAAATCTTCAATGCCGTGGAATAAACGTCAGCCAAAGCTTCAGG GTAAATCTGCAGAGACCAGAGAAAGCACACAGCTGAGCCCTGCAGATCTCACAGAAGGGAAACCCACAGA TCCCTCTAAGCTGGAAAGTCCGTCATTCACAGGAACTGGTGACACAGAAATAGCTCATGCAACTGAGGAT TTAGAGAATAATGGCAGTAAGAAAGATGGCGTGTGTGGTCCTCCTCCATCAAAGAAAATGAAGTTATTTG GATTTAAAGAAGATCCATTTGTATTTATTCCTGAAGATGACCCATTATTTCCACCTATTGAGAAATTTTA TGCTTTGGATCCTTCATTCCCAAGGATGAATTTGTTAACTCGGACTACAGAAGGGAAGAAAAGGCAGCTC TACATGGTTTCTAAGGAGTTGCGGAATGTGCTGCTGAATAACAGTGAGAAGATGAAGGTTATTAACACGG GGATCAAAGTCTGGTGTAGAAATAACAGCGGTGAAGAGTTTGACTGTGCTTTCCGGCTGGCACAGGAGGG AATATATACATTGTATCCATTTATTAACTCAAGAATTATTACTGTATCAATGGAAGATGTTAAGATACTG TTGACCCAGGAAAATCCCTTTTTTAGAAAACTCAGCAGTGAGACCTACAGTCAAGCAAAGGACCTGGCAA AGGGAAGCATCGTGCTGAAGTATGAACCAGATTCTGCGAATCCAGACGCTCTGCAGTGTCCCATCGTCTT ATGCGGATGGCGGGGAAAGGCCTCCATTCGAACTTTTGTGCCCAAGAATGAACGGCTTCATTATCTCAGG ATGATGGGGCTGGAGGTATTGGGAGAAAAGAAGAAGGAAGGGGTTATCCTCACAAATGAGAGTGCAGCCA GCACCGGACAGCCAGACAATGACGTGACTGAGGGACAGAGAGCAGGAGAGCCCAACAGCCCAGATGCAGA AGAGGCCAACAGTCCAGACGTGACAGCAGGCTGTGACCCGGCGGGGGTCCATCCACCCCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214459 representing NM_017755
Red=Cloning site Green=Tags(s) MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDAL REPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSP HLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGF VIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKN IDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSN ELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTG GFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATED LENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQL YMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKIL LTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLR MMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017755 |
ORF Size | 2301 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017755.4, NM_017755.5, NP_060225.4 |
RefSeq Size | 3089 bp |
RefSeq ORF | 2304 bp |
Locus ID | 54888 |
Domains | Nol1_Nop2_Sun |
MW | 86.3 kDa |
Gene Summary | This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC106395 | NSUN2 (untagged)-Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1 |
USD 760.00 |
|
RG214459 | NSUN2 (GFP-tagged) - Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1 |
USD 670.00 |
|
RC214459L1 | Lenti ORF clone of Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, Myc-DDK-tagged |
USD 1,008.00 |
|
RC214459L2 | Lenti ORF clone of Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, mGFP tagged |
USD 810.00 |
|
RC214459L3 | Lenti ORF clone of Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, Myc-DDK-tagged |
USD 810.00 |
|
RC214459L4 | Lenti ORF clone of Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, mGFP tagged |
USD 1,008.00 |
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