NSUN2 (NM_017755) Human Tagged ORF Clone

CAT#: RC214459L2

Lenti ORF clone of Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, mGFP tagged

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: NSUN2
• Synonyms: MISU; MRT5; SAKI; TRM4
• Vector: pLenti-C-mGFP
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC214459).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_017755
• ORF Size: 2301 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017755.4, NP_060225.4
• RefSeq Size: 3089
• RefSeq ORF: 2304
• Locus ID: 54888
• Domains: Nol1_Nop2_Sun
• MW: 86.3 kDa
• Gene Summary: This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]