KCNH3 (NM_012284) Human Tagged ORF Clone

CAT#: RC214540

KCNH3 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 3 (KCNH3)

Product Images

Western blot validation of overexpression lysate (Cat# LY415852) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214540 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNH3
• Synonyms: BEC1; ELK2; Kv12.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214540 representing NM_012284
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCATGCGGGGCCTCCTGGCGCCGCAGAACACCTTCCTGGACACCATCGCTACGCGCTTCGACG
  GCACGCACAGTAACTTCGTGCTGGGCAACGCCCAGGTGGCGGGGCTCTTCCCCGTGGTCTACTGCTCTGA
  TGGCTTCTGTGACCTCACGGGCTTCTCCCGGGCTGAGGTCATGCAGCGGGGCTGTGCCTGCTCCTTCCTT
  TATGGGCCAGACACCAGTGAGCTCGTCCGCCAACAGATCCGCAAGGCCCTGGACGAGCACAAGGAGTTCA
  AGGCTGAGCTGATCCTGTACCGGAAGAGCGGGCTCCCGTTCTGGTGTCTCCTGGATGTGATACCCATAAA
  GAATGAGAAAGGGGAGGTGGCTCTCTTCCTAGTCTCTCACAAGGACATCAGCGAAACCAAGAACCGAGGG
  GGCCCCGACAGATGGAAGGAGACAGGTGGTGGCCGGCGCCGATATGGCCGGGCACGATCCAAAGGCTTCA
  ATGCCAACCGGCGGCGGAGCCGGGCCGTGCTCTACCACCTGTCCGGGCACCTGCAGAAGCAGCCCAAGGG
  CAAGCACAAGCTCAATAAGGGGGTGTTTGGGGAGAAACCAAACTTGCCTGAGTACAAAGTAGCCGCCATC
  CGGAAGTCGCCCTTCATCCTGTTGCACTGTGGGGCACTGAGAGCCACCTGGGATGGCTTCATCCTGCTCG
  CCACACTCTATGTGGCTGTCACTGTGCCCTACAGCGTGTGTGTGAGCACAGCACGGGAGCCCAGTGCCGC
  CCGCGGCCCGCCCAGCGTCTGTGACCTGGCCGTGGAGGTCCTCTTCATCCTTGACATTGTGCTGAATTTC
  CGTACCACATTCGTGTCCAAGTCGGGCCAGGTGGTGTTTGCCCCAAAGTCCATTTGCCTCCACTACGTCA
  CCACCTGGTTCCTGCTGGATGTCATCGCAGCGCTGCCCTTTGACCTGCTACATGCCTTCAAGGTCAACGT
  GTACTTCGGGGCCCATCTGCTGAAGACGGTGCGCCTGCTGCGCCTGCTGCGCCTGCTTCCGCGGCTGGAC
  CGGTACTCGCAGTACAGCGCCGTGGTGCTGACACTGCTCATGGCCGTGTTCGCCCTGCTCGCGCACTGGG
  TCGCCTGCGTCTGGTTTTACATTGGCCAGCGGGAGATCGAGAGCAGCGAATCCGAGCTGCCTGAGATTGG
  CTGGCTGCAGGAGCTGGCCCGCCGACTGGAGACTCCCTACTACCTGGTGGGCCGGAGGCCAGCTGGAGGG
  AACAGCTCCGGCCAGAGTGACAACTGCAGCAGCAGCAGCGAGGCCAACGGGACGGGGCTGGAGCTGCTGG
  GCGGCCCGTCGCTGCGCAGCGCCTACATCACCTCCCTCTACTTCGCACTCAGCAGCCTCACCAGCGTGGG
  CTTCGGCAACGTGTCCGCCAACACGGACACCGAGAAGATCTTCTCCATCTGCACCATGCTCATCGGCGCC
  CTGATGCACGCGGTGGTGTTTGGGAACGTGACGGCCATCATCCAGCGCATGTACGCCCGCCGCTTTCTGT
  ACCACAGCCGCACGCGCGACCTGCGCGACTACATCCGCATCCACCGTATCCCCAAGCCCCTCAAGCAGCG
  CATGCTGGAGTACTTCCAGGCCACCTGGGCGGTGAACAATGGCATCGACACCACCGAGCTGCTGCAGAGC
  CTCCCTGACGAGCTGCGCGCAGACATCGCCATGCACCTGCACAAGGAGGTCCTGCAGCTGCCACTGTTTG
  AGGCGGCCAGCCGCGGCTGCCTGCGGGCACTGTCTCTGGCCCTGCGGCCCGCCTTCTGCACGCCGGGCGA
  GTACCTCATCCACCAAGGCGATGCCCTGCAGGCCCTCTACTTTGTCTGCTCTGGCTCCATGGAGGTGCTC
  AAGGGTGGCACCGTGCTCGCCATCCTAGGGAAGGGCGACCTGATCGGCTGTGAGCTGCCCCGGCGGGAGC
  AGGTGGTAAAGGCCAATGCCGACGTGAAGGGGCTGACGTACTGCGTCCTGCAGTGTCTGCAGCTGGCTGG
  CCTGCACGACAGCCTTGCGCTGTACCCCGAGTTTGCCCCGCGCTTCAGTCGTGGCCTCCGAGGGGAGCTC
  AGCTACAACCTGGGTGCTGGGGGAGGCTCTGCAGAGGTGGACACCAGCTCCCTGAGCGGCGACAATACCC
  TTATGTCCACGCTGGAGGAGAAGGAGACAGATGGGGAGCAGGGCCCCACGGTCTCCCCAGCCCCAGCTGA
  TGAGCCCTCCAGCCCCCTGCTGTCCCCTGGCTGCACCTCCTCATCCTCAGCTGCCAAGCTGCTATCCCCA
  CGTCGAACAGCACCCCGGCCTCGTCTAGGTGGCAGAGGGAGGCCAGGCAGGGCAGGGGCTTTGAAGGCTG
  AGGCTGGCCCCTCTGCTCCCCCACGGGCCCTAGAGGGGCTACGGCTGCCCCCCATGCCATGGAATGTGCC
  CCCAGATCTGAGCCCCAGGGTAGTAGATGGCATTGAAGACGGCTGTGGCTCGGACCAGCCCAAGTTCTCT
  TTCCGCGTGGGCCAGTCTGGCCCGGAATGTAGCAGCAGCCCCTCCCCTGGACCAGAGAGCGGCCTGCTCA
  CTGTTCCCCATGGGCCCAGCGAGGCAAGGAACACAGACACACTGGACAAGCTTCGGCAGGCGGTGACAGA
  GCTGTCAGAGCAGGTGCTGCAGATGCGGGAAGGACTGCAGTCACTTCGCCAGGCTGTGCAGCTTGTCCTG
  GCGCCCCACAGGGAGGGTCCGTGCCCTCGGGCATCGGGAGAGGGGCCGTGCCCAGCCAGCACCTCCGGGC
  TTCTGCAGCCTCTGTGTGTGGACACTGGGGCATCCTCCTACTGCCTGCAGCCCCCAGCTGGCTCTGTCTT
  GAGTGGGACTTGGCCCCACCCTCGTCCGGGGCCTCCTCCCCTCATGGCACCCTGGCCCTGGGGTCCCCCA
  GCGTCTCAGAGCTCCCCCTGGCCTCGAGCCACAGCTTTCTGGACCTCCACCTCAGACTCAGAGCCCCCTG
  CCTCAGGAGACCTCTGCTCTGAGCCCAGCACCCCTGCCTCCCCTCCTCCTTCTGAGGAAGGGGCTAGGAC
  TGGGCCCGCAGAGCCTGTGAGCCAGGCTGAGGCTACCAGCACTGGAGAGCCCCCACCAGGGTCAGGGGGC
  CTGGCCTTGCCCTGGGACCCCCACAGCCTGGAGATGGTGCTTATTGGCTGCCATGGCTCTGGCACAGTCC
  AGTGGACCCAGGAAGAAGGCACAGGGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214540 representing NM_012284
  Red=Cloning site Green=Tags(s)
  
  MPAMRGLLAPQNTFLDTIATRFDGTHSNFVLGNAQVAGLFPVVYCSDGFCDLTGFSRAEVMQRGCACSFL
  YGPDTSELVRQQIRKALDEHKEFKAELILYRKSGLPFWCLLDVIPIKNEKGEVALFLVSHKDISETKNRG
  GPDRWKETGGGRRRYGRARSKGFNANRRRSRAVLYHLSGHLQKQPKGKHKLNKGVFGEKPNLPEYKVAAI
  RKSPFILLHCGALRATWDGFILLATLYVAVTVPYSVCVSTAREPSAARGPPSVCDLAVEVLFILDIVLNF
  RTTFVSKSGQVVFAPKSICLHYVTTWFLLDVIAALPFDLLHAFKVNVYFGAHLLKTVRLLRLLRLLPRLD
  RYSQYSAVVLTLLMAVFALLAHWVACVWFYIGQREIESSESELPEIGWLQELARRLETPYYLVGRRPAGG
  NSSGQSDNCSSSSEANGTGLELLGGPSLRSAYITSLYFALSSLTSVGFGNVSANTDTEKIFSICTMLIGA
  LMHAVVFGNVTAIIQRMYARRFLYHSRTRDLRDYIRIHRIPKPLKQRMLEYFQATWAVNNGIDTTELLQS
  LPDELRADIAMHLHKEVLQLPLFEAASRGCLRALSLALRPAFCTPGEYLIHQGDALQALYFVCSGSMEVL
  KGGTVLAILGKGDLIGCELPRREQVVKANADVKGLTYCVLQCLQLAGLHDSLALYPEFAPRFSRGLRGEL
  SYNLGAGGGSAEVDTSSLSGDNTLMSTLEEKETDGEQGPTVSPAPADEPSSPLLSPGCTSSSSAAKLLSP
  RRTAPRPRLGGRGRPGRAGALKAEAGPSAPPRALEGLRLPPMPWNVPPDLSPRVVDGIEDGCGSDQPKFS
  FRVGQSGPECSSSPSPGPESGLLTVPHGPSEARNTDTLDKLRQAVTELSEQVLQMREGLQSLRQAVQLVL
  APHREGPCPRASGEGPCPASTSGLLQPLCVDTGASSYCLQPPAGSVLSGTWPHPRPGPPPLMAPWPWGPP
  ASQSSPWPRATAFWTSTSDSEPPASGDLCSEPSTPASPPPSEEGARTGPAEPVSQAEATSTGEPPPGSGG
  LALPWDPHSLEMVLIGCHGSGTVQWTQEEGTGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012284
• ORF Size: 3249 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012284.1, NM_012284.2, NP_036416.1
• RefSeq Size: 3853
• RefSeq ORF: 3252
• Locus ID: 23416
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 116.9 kDa
• Gene Summary: The protein encoded by this gene is a voltage-gated potassium channel alpha subunit predominantly expressed in the forebrain. Studies in mice have found that cognitive function increases when this gene is knocked out. In humans, the encoded protein has been shown to be capable of binding glycoprotein 120 of the human immunodeficiency virus type 1 (HIV-1) envelope. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]