RASA1 (NM_022650) Human Tagged ORF Clone

CAT#: RC214699

RASA1 (Myc-DDK-tagged)-Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RASA1
• Synonyms: CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214699 representing NM_022650
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGGTGGTATCACGGAAAACTTGACAGAACGATAGCAGAAGAACGCCTCAGGCAGGCAGGGAAGT
  CTGGCAGTTATCTTATAAGAGAGAGTGATCGGAGGCCAGGGTCCTTTGTACTTTCATTTCTTAGCCAGAT
  GAATGTTGTCAACCATTTTAGGATTATTGCTATGTGTGGAGATTACTACATTGGTGGAAGACGTTTTTCT
  TCACTGTCAGACCTAATAGGTTATTACAGTCATGTTTCTTGTTTGCTTAAAGGAGAAAAATTACTTTACC
  CAGTTGCACCACCAGAGCCAGTAGAAGATAGAAGGCGTGTACGAGCTATTCTACCTTACACAAAAGTACC
  AGACACTGATGAAATAAGTTTCTTAAAAGGAGATATGTTCATTGTTCATAATGAATTAGAAGATGGATGG
  ATGTGGGTTACAAATTTAAGAACAGATGAACAAGGCCTTATTGTTGAAGACCTAGTAGAAGAGGTGGGCC
  GGGAAGAAGATCCACATGAAGGAAAAATATGGTTCCATGGGAAGATTTCCAAACAGGAAGCTTATAATTT
  ACTAATGACAGTTGGTCAAGTCTGCAGTTTTCTTGTGAGGCCCTCAGATAATACTCCTGGCGATTATTCA
  CTTTATTTCCGGACCAATGAAAATATTCAGCGATTTAAAATATGTCCAACGCCAAACAATCAGTTTATGA
  TGGGAGGCCGGTATTATAACAGCATTGGGGACATCATAGATCACTATCGAAAAGAACAGATTGTTGAAGG
  ATATTATCTTAAGGAACCTGTACCAATGCAGGATCAAGAACAAGTACTCAATGACACAGTGGATGGCAAG
  GAAATCTATAATACCATCCGTCGTAAAACAAAGGATGCCTTTTATAAAAACATTGTTAAGAAAGGTTATC
  TTCTGAAAAAGGGCAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTAGAGGGTAGTGATGCCCAACT
  TATTTATTTTGAAAGCGAAAAACGAGCTACCAAACCAAAAGGATTAATAGATCTCAGTGTATGTTCTGTC
  TATGTCGTTCATGATAGTCTCTTTGGCAGGCCAAACTGTTTTCAGATAGTAGTTCAGCACTTTAGTGAAG
  AACATTACATCTTTTACTTTGCAGGAGAAACTCCAGAACAAGCAGAGGATTGGATGAAAGGTCTGCAGGC
  ATTTTGCAATTTACGGAAAAGTAGTCCAGGGACATCCAATAAACGCCTTCGTCAGGTCAGCAGCCTTGTT
  TTACATATTGAAGAAGCCCATAAACTCCCAGTAAAACATTTTACTAATCCATATTGTAACATCTACCTGA
  ATAGTGTCCAAGTAGCAAAAACTCATGCAAGGGAAGGGCAAAACCCAGTATGGTCAGAAGAGTTTGTCTT
  TGATGATCTTCCTCCTGACATCAATAGATTTGAAATAACTCTTAGTAATAAAACAAAGAAAAGCAAAGAT
  CCTGATATCTTATTTATGCGCTGCCAGTTGAGCCGATTACAGAAAGGGCATGCCACAGATGAATGGTTTC
  TGCTCAGCTCCCATATACCATTAAAAGGTATTGAACCAGGGTCCCTGCGTGTTCGAGCACGATACTCTAT
  GGAAAAAATCATGCCAGAAGAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAAAAGGAACTTCATGTA
  GTCTATGCTTTATCACATGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCTACTGAGGATTTTTC
  TTCACGAAAAGCTTGAATCGTTGTTGTTATGCACACTAAATGACAGAGAAATAAGCATGGAAGATGAAGC
  CACTACCCTATTTCGAGCCACAACACTTGCAAGCACCTTGATGGAGCAGTATATGAAAGCCACTGCTACA
  CAGTTTGTTCATCATGCTTTGAAAGACTCTATTTTAAAGATAATGGAAAGCAAGCAGTCTTGTGAGTTAA
  GTCCATCAAAGTTAGAAAAAAATGAAGATGTGAACACTAATTTAACACACCTATTGAACATACTTTCAGA
  GCTTGTGGAGAAAATATTCATGGCTTCAGAAATACTTCCACCGACATTGAGATATATTTATGGGTGTTTA
  CAGAAATCTGTTCAGCATAAGTGGCCTACAAATACCACCATGAGAACAAGAGTTGTTAGTGGTTTTGTTT
  TTCTTCGACTCATCTGTCCTGCCATCCTGAATCCACGGATGTTCAATATCATCTCAGATTCTCCATCTCC
  TATTGCTGCAAGAACACTGATATTAGTGGCTAAATCTGTGCAGAACTTAGCAAATCTTGTGGAATTTGGA
  GCTAAGGAGCCCTACATGGAAGGTGTCAATCCATTCATCAAAAGCAACAAACATCGTATGATCATGTTTT
  TAGATGAACTTGGGAATGTACCTGAACTTCCGGACACTACAGAGCATTCTAGAACGGACCTGTCCCGTGA
  TTTAGCAGCATTGCATGAGATTTGCGTGGCTCATTCAGATGAACTTCGAACGCTCAGTAATGAGCGTGGT
  GCACAGCAGCACGTATTGAAAAAGCTTCTGGCTATAACAGAACTGCTTCAACAAAAACAAAACCAGTATA
  CAAAAACCAATGATGTCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214699 representing NM_022650
  Red=Cloning site Green=Tags(s)
  
  MKGWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQMNVVNHFRIIAMCGDYYIGGRRFS
  SLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGW
  MWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYS
  LYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDTVDGK
  EIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSV
  YVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLV
  LHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKD
  PDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHV
  VYALSHVCGQDRTLLASILLRIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATAT
  QFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLTHLLNILSELVEKIFMASEILPPTLRYIYGCL
  QKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLILVAKSVQNLANLVEFG
  AKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERG
  AQQHVLKKLLAITELLQQKQNQYTKTNDVR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022650
• ORF Size: 2610 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022650.1, NM_022650.2, NP_072179.1
• RefSeq Size: 3796 bp
• RefSeq ORF: 2613 bp
• Locus ID: 5921
• Cytogenetics: 5q14.3
• Domains: C2, SH2, SH3, PH, RasGAP, VPS9
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, MAPK signaling pathway
• MW: 100.4 kDa
• Gene Summary: 'The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]'