RASA1 (NM_022650) Human Untagged Clone

CAT#: SC314846

RASA1 (untagged)-Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASA1
• Synonyms: CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022650, the custom clone sequence may differ by one or more nucleotides
  ATGAAGGGGTGGTATCACGGAAAACTTGACAGAACGATAGCAGAAGAACGCCTCAGGCAG
  GCAGGGAAGTCTGGCAGTTATCTTATAAGAGAGAGTGATCGGAGGCCAGGGTCCTTTGTA
  CTTTCATTTCTTAGCCAGATGAATGTTGTCAACCATTTTAGGATTATTGCTATGTGTGGA
  GATTACTACATTGGTGGAAGACGTTTTTCTTCACTGTCAGACCTAATAGGTTATTACAGT
  CATGTTTCTTGTTTGCTTAAAGGAGAAAAATTACTTTACCCAGTTGCACCACCAGAGCCA
  GTAGAAGATAGAAGGCGTGTACGAGCTATTCTACCTTACACAAAAGTACCAGACACTGAT
  GAAATAAGTTTCTTAAAAGGAGATATGTTCATTGTTCATAATGAATTAGAAGATGGATGG
  ATGTGGGTTACAAATTTAAGAACAGATGAACAAGGCCTTATTGTTGAAGACCTAGTAGAA
  GAGGTGGGCCGGGAAGAAGATCCACATGAAGGAAAAATATGGTTCCATGGGAAGATTTCC
  AAACAGGAAGCTTATAATTTACTAATGACAGTTGGTCAAGTCTGCAGTTTTCTTGTGAGG
  CCCTCAGATAATACTCCTGGCGATTATTCACTTTATTTCCGGACCAATGAAAATATTCAG
  CGATTTAAAATATGTCCAACGCCAAACAATCAGTTTATGATGGGAGGCCGGTATTATAAC
  AGCATTGGGGACATCATAGATCACTATCGAAAAGAACAGATTGTTGAAGGATATTATCTT
  AAGGAACCTGTACCAATGCAGGATCAAGAACAAGTACTCAATGACACAGTGGATGGCAAG
  GAAATCTATAATACCATCCGTCGTAAAACAAAGGATGCCTTTTATAAAAACATTGTTAAG
  AAAGGTTATCTTCTGAAAAAGGGCAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTA
  GAGGGTAGTGATGCCCAACTTATTTATTTTGAAAGCGAAAAACGAGCTACCAAACCAAAA
  GGATTAATAGATCTCAGTGTATGTTCTGTCTATGTCGTTCATGATAGTCTCTTTGGCAGG
  CCAAACTGTTTTCAGATAGTAGTTCAGCACTTTAGTGAAGAACATTACATCTTTTACTTT
  GCAGGAGAAACTCCAGAACAAGCAGAGGATTGGATGAAAGGTCTGCAGGCATTTTGCAAT
  TTACGGAAAAGTAGTCCAGGGACATCCAATAAACGCCTTCGTCAGGTCAGCAGCCTTGTT
  TTACATATTGAAGAAGCCCATAAACTCCCAGTAAAACATTTTACTAATCCATATTGTAAC
  ATCTACCTGAATAGTGTCCAAGTAGCAAAAACTCATGCAAGGGAAGGGCAAAACCCAGTA
  TGGTCAGAAGAGTTTGTCTTTGATGATCTTCCTCCTGACATCAATAGATTTGAAATAACT
  CTTAGTAATAAAACAAAGAAAAGCAAAGATCCTGATATCTTATTTATGCGCTGCCAGTTG
  AGCCGATTACAGAAAGGGCATGCCACAGATGAATGGTTTCTGCTCAGCTCCCATATACCA
  TTAAAAGGTATTGAACCAGGGTCCCTGCGTGTTCGAGCACGATACTCTATGGAAAAAATC
  ATGCCAGAAGAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAAAAGGAACTTCATGTA
  GTCTATGCTTTATCACATGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCTACTG
  AGGATTTTTCTTCACGAAAAGCTTGAATCGTTGTTGTTATGCACACTAAATGACAGAGAA
  ATAAGCATGGAAGATGAAGCCACTACCCTATTTCGAGCCACAACACTTGCAAGCACCTTG
  ATGGAGCAGTATATGAAAGCCACTGCTACACAGTTTGTTCATCATGCTTTGAAAGACTCT
  ATTTTAAAGATAATGGAAAGCAAGCAGTCTTGTGAGTTAAGTCCATCAAAGTTAGAAAAA
  AATGAAGATGTGAACACTAATTTAACACACCTATTGAACATACTTTCAGAGCTTGTGGAG
  AAAATATTCATGGCTTCAGAAATACTTCCACCGACATTGAGATATATTTATGGGTGTTTA
  CAGAAATCTGTTCAGCATAAGTGGCCTACAAATACCACCATGAGAACAAGAGTTGTTAGT
  GGTTTTGTTTTTCTTCGACTCATCTGTCCTGCCATCCTGAATCCACGGATGTTCAATATC
  ATCTCAGATTCTCCATCTCCTATTGCTGCAAGAACACTGATATTAGTGGCTAAATCTGTG
  CAGAACTTAGCAAATCTTGTGGAATTTGGAGCTAAGGAGCCCTACATGGAAGGTGTCAAT
  CCATTCATCAAAAGCAACAAACATCGTATGATCATGTTTTTAGATGAACTTGGGAATGTA
  CCTGAACTTCCGGACACTACAGAGCATTCTAGAACGGACCTGTCCCGTGATTTAGCAGCA
  TTGCATGAGATTTGCGTGGCTCATTCAGATGAACTTCGAACGCTCAGTAATGAGCGTGGT
  GCACAGCAGCACGTATTGAAAAAGCTTCTGGCTATAACAGAACTGCTTCAACAAAAACAA
  AACCAGTATACAAAAACCAATGATGTCAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_022650
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022650.1, NP_072179.1
• RefSeq Size: 3757 bp
• RefSeq ORF: 2613 bp
• Locus ID: 5921
• Cytogenetics: 5q14.3
• Domains: C2, SH2, SH3, PH, RasGAP, VPS9
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]'
  Transcript Variant: This variant (2) includes an alternatively spliced exon in the 5' portion of the transcript, allowing for translation from an alternative initiation codon and resulting in the shorter isoform (2) which is missing the hydrophobic amino terminus found in isoform 1.