Contactin 1 (CNTN1) (NM_001843) Human Tagged ORF Clone

CAT#: RC214706

CNTN1 (Myc-DDK-tagged)-Human contactin 1 (CNTN1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNTN1
• Synonyms: F3; GP135; MYPCN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214706 representing NM_001843
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAATGTGGTTGCTGGTCAGTCATCTTGTGATAATATCTATTACTACCTGTTTAGCAGAGTTTACAT
  GGTATAGAAGATATGGTCATGGAGTTTCTGAGGAAGACAAAGGATTTGGACCAATTTTTGAAGAGCAGCC
  AATCAATACCATTTATCCAGAGGAATCACTGGAAGGAAAAGTCTCACTCAACTGTAGGGCACGAGCCAGC
  CCTTTCCCGGTTTACAAATGGAGAATGAATAATGGGGACGTTGATCTCACAAGTGATCGATACAGTATGG
  TAGGAGGAAACCTTGTTATCAACAACCCTGACAAACAGAAAGATGCTGGAATATACTACTGTTTAGCATC
  TAATAACTACGGGATGGTCAGAAGCACTGAAGCAACCCTGAGCTTTGGATATCTTGATCCTTTCCCACCT
  GAGGAACGTCCTGAGGTCAGAGTAAAAGAAGGGAAAGGAATGGTGCTTCTCTGTGACCCCCCATACCATT
  TTCCAGATGATCTTAGCTATCGCTGGCTTCTAAATGAATTTCCTGTATTTATCACAATGGATAAACGGCG
  ATTTGTGTCTCAGACAAATGGCAATCTCTACATTGCAAATGTTGAGGCTTCCGACAAAGGCAATTATTCC
  TGCTTTGTTTCCAGTCCTTCTATTACAAAGAGCGTGTTCAGCAAATTCATCCCACTCATTCCAATACCTG
  AACGAACAACAAAACCATATCCTGCTGATATTGTAGTTCAGTTCAAGGATGTATATGCATTGATGGGCCA
  AAATGTGACCTTAGAATGTTTTGCACTTGGAAATCCTGTTCCGGATATCCGATGGCGGAAGGTTCTAGAA
  CCAATGCCAAGCACTGCTGAGATTAGCACCTCTGGGGCTGTTCTTAAGATCTTCAATATTCAGCTAGAAG
  ATGAAGGCATCTATGAATGTGAGGCTGAGAACATTAGAGGAAAGGATAAACATCAAGCAAGAATTTATGT
  TCAAGCATTCCCTGAGTGGGTAGAACACATCAATGACACAGAGGTGGACATAGGCAGTGATCTCTACTGG
  CCTTGTGTGGCCACAGGAAAGCCCATCCCTACAATCCGATGGTTGAAAAATGGATATGCGTATCATAAAG
  GGGAATTAAGACTGTATGATGTGACTTTTGAAAATGCCGGAATGTATCAGTGCATAGCTGAAAACACATA
  TGGAGCCATTTATGCAAATGCTGAGTTGAAGATCTTGGCGTTGGCTCCAACTTTTGAAATGAATCCTATG
  AAGAAAAAGATCCTGGCTGCTAAAGGTGGAAGGGTGATAATTGAATGCAAACCTAAAGCTGCACCGAAAC
  CAAAGTTTTCATGGAGTAAAGGGACAGAGTGGCTTGTCAATAGCAGCAGAATACTCATTTGGGAAGATGG
  TAGCTTGGAAATCAATAACATTACAAGGAATGATGGAGGTATCTATACATGCTTTGCAGAAAATAACAGA
  GGGAAAGCTAATAGCACTGGAACCCTTGTTATCACAGATCCTACGCGAATTATATTGGCCCCAATTAATG
  CCGATATCACAGTTGGAGAAAACGCCACCATGCAGTGTGCTGCGTCCTTTGATCCTGCCTTGGATCTCAC
  ATTTGTTTGGTCCTTCAATGGCTATGTGATCGATTTTAACAAAGAGAATATTCACTACCAGAGGAATTTT
  ATGCTGGATTCCAATGGGGAATTACTAATCCGAAATGCGCAGCTGAAACATGCTGGAAGATACACATGCA
  CTGCCCAGACAATTGTGGACAATTCTTCAGCTTCAGCTGACCTTGTAGTGAGAGGCCCTCCAGGCCCTCC
  AGGTGGTCTGAGAATAGAAGACATTAGAGCCACTTCTGTGGCACTTACTTGGAGCCGTGGTTCAGACAAT
  CATAGTCCTATTTCTAAATACACTATCCAGACCAAGACTATTCTTTCAGATGACTGGAAAGATGCAAAGA
  CAGATCCCCCAATTATTGAAGGAAATATGGAGGCAGCAAGAGCAGTGGACTTAATCCCATGGATGGAGTA
  TGAATTCCGCGTGGTAGCAACCAATACACTGGGTAGAGGAGAGCCCAGTATACCATCTAACAGAATTAAA
  ACAGACGGTGCTGCACCAAATGTGGCTCCTTCAGATGTAGGAGGTGGAGGTGGAAGAAACAGAGAGCTGA
  CCATAACATGGGCGCCTTTGTCAAGAGAATACCACTATGGCAACAATTTTGGTTACATAGTGGCATTTAA
  GCCATTTGATGGAGAAGAATGGAAAAAAGTCACAGTTACTAATCCTGATACTGGCCGATATGTCCATAAA
  GATGAAACCATGAGCCCTTCCACTGCATTTCAAGTTAAAGTCAAGGCCTTCAACAACAAAGGAGATGGAC
  CTTACAGCCTAGTAGCAGTCATTAATTCAGCACAAGACGCTCCCAGTGAAGCCCCAACAGAAGTAGGTGT
  AAAAGTCTTATCATCTTCTGAGATATCTGTTCATTGGGAACATGTTTTAGAAAAAATAGTGGAAAGCTAT
  CAGATTCGGTATTGGGCTGCCCATGACAAAGAAGAAGCTGCAAACAGAGTTCAAGTCACCAGCCAAGAGT
  ACTCGGCCAGGCTCGAGAACCTTCTGCCAGACACCCAGTATTTTATAGAAGTCGGGGCCTGCAATAGTGC
  AGGGTGTGGACCTCCAAGTGACATGATTGAGGCTTTCACCAAGAAAGCACCTCCTAGCCAGCCTCCAAGG
  ATCATCAGTTCAGTAAGGTCTGGTTCACGCTATATAATCACCTGGGATCATGTCGTTGCACTATCAAATG
  AATCTACAGTGACGGGATATAAGGTACTCTACAGACCTGATGGCCAGCATGATGGCAAGCTGTATTCAAC
  TCACAAACACTCCATAGAAGTCCCAATCCCCAGAGATGGAGAATACGTTGTGGAGGTTCGCGCGCACAGT
  GATGGAGGAGATGGAGTGGTGTCTCAAGTCAAAATTTCAGGTGCACCCACCCTATCCCCAAGTCTTCTCG
  GCTTACTGCTGCCTGCCTTTGGCATCCTTGTCTACTTGGAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214706 representing NM_001843
  Red=Cloning site Green=Tags(s)
  
  MKMWLLVSHLVIISITTCLAEFTWYRRYGHGVSEEDKGFGPIFEEQPINTIYPEESLEGKVSLNCRARAS
  PFPVYKWRMNNGDVDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPP
  EERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYS
  CFVSSPSITKSVFSKFIPLIPIPERTTKPYPADIVVQFKDVYALMGQNVTLECFALGNPVPDIRWRKVLE
  PMPSTAEISTSGAVLKIFNIQLEDEGIYECEAENIRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYW
  PCVATGKPIPTIRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTYGAIYANAELKILALAPTFEMNPM
  KKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNR
  GKANSTGTLVITDPTRIILAPINADITVGENATMQCAASFDPALDLTFVWSFNGYVIDFNKENIHYQRNF
  MLDSNGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDN
  HSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMEAARAVDLIPWMEYEFRVVATNTLGRGEPSIPSNRIK
  TDGAAPNVAPSDVGGGGGRNRELTITWAPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHK
  DETMSPSTAFQVKVKAFNNKGDGPYSLVAVINSAQDAPSEAPTEVGVKVLSSSEISVHWEHVLEKIVESY
  QIRYWAAHDKEEAANRVQVTSQEYSARLENLLPDTQYFIEVGACNSAGCGPPSDMIEAFTKKAPPSQPPR
  IISSVRSGSRYIITWDHVVALSNESTVTGYKVLYRPDGQHDGKLYSTHKHSIEVPIPRDGEYVVEVRAHS
  DGGDGVVSQVKISGAPTLSPSLLGLLLPAFGILVYLEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001843
• ORF Size: 3054 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001843.1, NM_001843.2, NM_001843.3, NP_001834.2
• RefSeq Size: 3427 bp
• RefSeq ORF: 3057 bp
• Locus ID: 1272
• Cytogenetics: 12q12
• Domains: ig, IGc2, IG, FN3
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 113.32 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]'