SRP72 (NM_006947) Human Tagged ORF Clone
CAT#: RC214727
SRP72 (Myc-DDK-tagged)-Human signal recognition particle 72kDa (SRP72)
"NM_006947" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SRP72 |
Synonyms | BMFF; BMFS1; HEL103 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214727 representing NM_006947
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTGAAGTGAACCGGTATGGCCAGA ACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGATACTACAGATCAACAAAGATGACGTAACTGC CCTGCATTGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTCAAGGAAGCTTTGAATGTCATCAATACT CACACCAAAGTGTTAGCCAATAACTCTCTCTCCTTTGAAAAGGCATATTGCGAGTACAGGCTGAACAGAA TTGAGAATGCCTTGAAGACAATAGAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTATGGACA AGTGTTATATCGTTTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCCGAAACTCCCAA GATGATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAGCTCAAAGCAATTGGGAAAAAG TGGTTCCAGAGAACCTGGGCCTCCAAGAAGGCACACATGAGCTGTGCTACAACACTGCATGTGCACTGAT AGGCCAAGGCCAGCTGAACCAGGCCATGAAAATCCTACAAAAAGCTGAAGATCTTTGCCGCCGTTCATTA TCAGAAGACACTGATGGGACTGAGGAAGACCCACAGGCAGAACTGGCCATCATTCATGGTCAGATGGCTT ATATTCTGCAGCTTCAGGGTCGAACAGAGGAGGCTTTGCAACTTTACAATCAAATAATAAAACTAAAACC AACAGATGTGGGATTACTAGCTGTAATTGCAAATAACATCATTACCATTAACAAGGACCAAAATGTCTTT GACTCCAAGAAGAAAGTGAAATTAACCAATGCGGAAGGAGTAGAGTTTAAGCTTTCCAAGAAACAACTAC AAGCTATAGAATTTAACAAAGCTTTACTTGCTATGTACACAAACCAGGCTGAACAATGCCGCAAAATATC TGCCAGTTTACAGTCCCAAAGTCCCGAGCATCTCTTACCTGTGTTAATCCAAGCTGCCCAGCTCTGCCGT GAAAAGCAGCACACAAAAGCAATAGAGCTGCTTCAGGAATTTTCAGATCAGCATCCAGAAAATGCAGCTG AAATTAAGCTGACCATGGCACAGTTGAAAATTTCTCAAGGTAATATTTCTAAAGCATGTCTAATATTGAG AAGCATAGAGGAGTTAAAGCATAAACCAGGCATGGTATCTGCATTAGTTACCATGTATAGCCATGAAGAA GATATTGATAGTGCCATTGAGGTCTTCACACAAGCTATCCAGTGGTATCAAAACCATCAGCCAAAATCTC CTGCTCATTTGTCCTTGATAAGAGAAGCTGCAAACTTCAAACTCAAATATGGGCGGAAGAAGGAGGCAAT TAGTGACCTACAACAGCTGTGGAAACAAAATCCAAAAGATATTCACACCCTGGCACAGCTTATTTCTGCT TACTCACTTGTAGATCCAGAGAAAGCCAAAGCTCTTAGTAAACACTTGCCATCGTCAGATAGTATGTCTC TAAAAGTAGATGTTGAGGCTCTTGAAAATTCTGCTGGTGCTACATACATTCGGAAGAAGGGTGGAAAAGT TACTGGAGATAGTCAACCAAAGGAACAAGGACAGGGAGATTTGAAAAAGAAGAAAAAGAAAAAGAAGGGA AAATTGCCTAAGAATTATGACCCAAAAGTTACCCCAGATCCAGAAAGATGGCTGCCAATGCGAGAACGTT CTTACTACCGGGGAAGAAAGAAGGGTAAAAAGAAGGATCAGATTGGAAAAGGGACCCAGGGAGCAACTGC AGGAGCTTCATCTGAACTGGATGCCAGTAAAACTGTGAGCAGCCCACCCACCTCCCCAAGACCTGGCAGT GCTGCAACAGTATCTGCCTCTACAAGTAACATCATACCCCCAAGACACCAGAAACCTGCAGGGGCTCCAG CAACAAAAAAGAAACAGCAACAGAAAAAGAAGAAAGGTGGAAAAGGTGGCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214727 representing NM_006947
Red=Cloning site Green=Tags(s) MASGGSGGVSVPALWSEVNRYGQNGDFTRALKTVNKILQINKDDVTALHCKVVCLIQNGSFKEALNVINT HTKVLANNSLSFEKAYCEYRLNRIENALKTIESANQQTDKLKELYGQVLYRLERYDECLAVYRDLVRNSQ DDYDEERKTNLSAVVAAQSNWEKVVPENLGLQEGTHELCYNTACALIGQGQLNQAMKILQKAEDLCRRSL SEDTDGTEEDPQAELAIIHGQMAYILQLQGRTEEALQLYNQIIKLKPTDVGLLAVIANNIITINKDQNVF DSKKKVKLTNAEGVEFKLSKKQLQAIEFNKALLAMYTNQAEQCRKISASLQSQSPEHLLPVLIQAAQLCR EKQHTKAIELLQEFSDQHPENAAEIKLTMAQLKISQGNISKACLILRSIEELKHKPGMVSALVTMYSHEE DIDSAIEVFTQAIQWYQNHQPKSPAHLSLIREAANFKLKYGRKKEAISDLQQLWKQNPKDIHTLAQLISA YSLVDPEKAKALSKHLPSSDSMSLKVDVEALENSAGATYIRKKGGKVTGDSQPKEQGQGDLKKKKKKKKG KLPKNYDPKVTPDPERWLPMRERSYYRGRKKGKKKDQIGKGTQGATAGASSELDASKTVSSPPTSPRPGS AATVSASTSNIIPPRHQKPAGAPATKKKQQQKKKKGGKGGW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006947 |
ORF Size | 2013 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006947.1, NM_006947.2, NM_006947.3, NP_008878.2 |
RefSeq Size | 3852 bp |
RefSeq ORF | 2016 bp |
Locus ID | 6731 |
Cytogenetics | 4q12 |
Domains | TPR |
Protein Pathways | Protein export |
MW | 74.4 kDa |
Gene Summary | 'This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC115795 | SRP72 (untagged)-Human signal recognition particle 72kDa (SRP72) |
USD 1,370.00 |
|
RG214727 | SRP72 (GFP-tagged) - Human signal recognition particle 72kDa (SRP72) |
USD 830.00 |
|
RC214727L1 | Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), Myc-DDK-tagged |
USD 1,176.00 |
|
RC214727L2 | Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), mGFP tagged |
USD 950.00 |
|
RC214727L3 | Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), Myc-DDK-tagged |
USD 950.00 |
|
RC214727L4 | Lenti ORF clone of Human signal recognition particle 72kDa (SRP72), mGFP tagged |
USD 950.00 |
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