SRP72 (NM_006947) Human Untagged Clone

CAT#: SC115795

SRP72 (untagged)-Human signal recognition particle 72kDa (SRP72)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRP72
• Synonyms: BMFF; BMFS1; HEL103
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006947 edited
  GCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGT
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTCTCCTCCAAGATG
  GCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTGAAGTGAACCGGTAT
  GGCCAGAACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGATACTACAGATCAAC
  AAAGATGACGTAACTGCCCTGCATTGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTC
  AAGGAAGCTTTGAATGTCATCAATACTCACACCAAAGTGTTAGCCAATAACTCTCTCTCC
  TTTGAAAAGGCATATTGCGAGTACAGGCTGAACAGAATTGAGAATGCCTTGAAGACAATA
  GAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTATGGACAAGTGTTATATCGT
  TTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCCGAAACTCCCAAGAT
  GATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAGCTCAAAGCAATTGG
  GAAAAAGTGGTTCCAGAGAACCTGGGCCTCCAAGAAGGCACACATGAGCTGTGCTACAAC
  ACTGCATGTGCACTGATAGGCCAAGGCCAGCTGAACCAGGCCATGAAAATCCTACAAAAA
  GCTGAAGATCTTTGCCGCCGTTCATTATCAGAAGACACTGATGGGACTGAGGAAGACCCA
  CAGGCAGAACTGGCCATCATTCATGGTCAGATGGCTTATATTCTGCAGCTTCAGGGTCGA
  ACAGAGGAGGCTTTGCAACTTTACAATCAAATAATAAAACTAAAACCAACAGATGTGGGA
  TTACTAGCTGTAATTGCAAATAACATCATTACCATTAACAAGGACCAAAATGTCTTTGAC
  TCCAAGAAGAAAGTGAAATTAACCAATGCGGAAGGAGTAGAGTTTAAGCTTTCCAAGAAA
  CAACTACAAGCTATAGAATTTAACAAAGCTTTACTTGCTATGTACACAAACCAGGCTGAA
  CAATGCCGCAAAATATCTGCCAGTTTACAGTCCCAAAGTCCCGAGCATCTCTTACCTGTG
  TTAATCCAAGCTGCCCAGCTCTGCCGTGAAAAGCAGCACACAAAAGCAATAGAGCTGCTT
  CAGGAATTTTCAGATCAGCATCCAGAAAATGCAGCTGAAATTAAGCTGACCATGGCACAG
  TTGAAAATTTCTCAAGGTAATATTTCTAAAGCATGTCTAATATTGAGAAGCATAGAGGAG
  TTAAAGCATAAACCAGGCATGGTATCTGCATTAGTTACCATGTATAGCCATGAAGAAGAT
  ATTGATAGTGCCATTGAGGTCTTCACACAAGCTATCCAGTGGTATCAAAACCATCAGCCA
  AAATCTCCTGCTCATTTGTCCTTGATAAGAGAAGCTGCAAACTTCAAACTCAAATATGGG
  CGGAAGAAGGAGGCAATTAGTGACCTACAACAGCTGTGGAAACAAAATCCAAAAGATATT
  CACACCCTGGCACAGCTTATTTCTGCTTACTCACTTGTAGATCCAGAGAAAGCCAAAGCT
  CTTAGTAAACACTTGCCATCGTCAGATAGTATGTCTCTAAAAGTAGATGTTGAGGCTCTT
  GAAAATTCTGCTGGTGCTACATACATTCGGAAGAAGGGTGGAAAAGTTACTGGAGATAGT
  CAACCAAAGGAACAAGGACAGGGAGATTTGAAAAAGAAGAAAAAGAAAAAGAAGGGAAAA
  TTGCCTAAGAATTATGACCCAAAAGTTACCCCAGATCCAGAAAGATGGCTGCCAATGCGA
  GAACGTTCTTACTACCGGGGAAGAAAGAAGGGTAAAAAGAAGGATCAGATTGGAAAAGGG
  ACCCAGGGAGCAACTGCAGGAGCTTCATCTGAACTGGATGCCAGTAAAACTGTGAGCAGC
  CCACCCACCTCCCCAAGACCTGGCAGTGCTGCAACAGTATCTGCCTCTACAAGTAACATC
  ATACCCCCAAGACACCAGAAACCTGCAGGGGCTCCAGCAACAAAAAAGAAACAGCAACAG
  AAAAAGAAGAAAGGTGGAAAAGGTGGCTGGTGATGAGAATATTCTTGTTGCAGGCTGTTT
  TTAAACTAGTGTCAGTGACACTAGGAATATAATAAAGGTAACACAGCAAGAAGCACAGAA
  CTACTCCCTCTTCATCTCCATATTTTCATAATTTCTTGTGTTTCAAATAGGGAAACATCT
  TCCTCAAAGTCTGCCTAGTGAGATATGGCCTACTGGTTGCCTCATAGCTTTGTACAGATT
  ATGAGGACTGAAAATAATTGGGCATTTACCCATCTTGGTATCTGTTGTATCCTTTATCTG
  TGTGTGCTGATTTGATCTTTTTTCAGTTTCACATACCTTATCTAAGGTTTCCCAGGATTT
  AAACAGAAACTACTT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006947 unedited
  TCACCAATTTGGTATACGACTCACTATAGNNGGCGGCCGCGNAATTCGGCACGAGGCCTC
  GTCTCCTCCAAATGGCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTG
  AAGTGAACCGGTATGGCCAGAACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGA
  TACTACAGATCAACAAAGATGACGTAACTGCCCTGCATTGTAAAGTGGTATGCCTTATCC
  AGAATGGAAGTTTCAAGGAAGCTTTGAATGTCATCAATACTCACACCAAAGTGTTAGCCA
  ATAACTCTCTCTCCTTTGAAAAGGCATATTGCGAGTACAGGCTGAACAGAATTGAGAATG
  CCTTGAAGACAATAGAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTATGGAC
  AAGTGTTATATCGTTTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCC
  GAAACTCCCAAGATGATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAG
  CTCAAAGCAATTGGGAAAAAGTGGTTCCAGAGAACCTGNGCCTCCAAGAAGGCACACATG
  AGCTGTGCTACAACACTGCATGTGCACTGATAGGCCAAGGCCAGCTGAACCAGGCCATGA
  AAATCCTACATAAAGCTGAAGATCCTTTGCCGCCGTTCATTATCAGAAGACACTGATGGG
  ACTGAAGAAGACCCACAGGCAGAACTGGCCATCATTCATGGGCAGAAGGCTTATATTCTG
  CAGCTTCAGGGTCGAACAGAGGGAGCTTTGCAACTTTACATTAAATAATAAAACTAAACC
  CACCGATGTGGGATTACTAGCTGTAATTGCAATAACATTCTTCCCTTAACACGAACCAAA
  TGTCTTTTACTCCCAGAACAAGTGAAATTACCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006947 unedited
  CCCCCCCGGGCCCCCCCCCNCNTTTTACCCCCNNNTTTTTGGGCANNCCTTTCTTTTTTT
  TGTGGGCCTNCCTTTNATGNAANACATNCATGTATTAAAAAGTCCCTCTTTATCACATGA
  TATTTCTAAGCAGNATATTTAGAATGCTCAGAAGATTCCACTTTCAGTCTCCTAAGAACA
  TAATTTAAAGTGGGAACTTGAACAGNAAACAAGTATCTTCAGACTCCAGCTGAAATCATA
  GAAGTAGTTTCTGTTTAAATCCTGGGAAACCTTAGATAAGGTATGTGAAACTGAAAAAAG
  ATCAAATCAGCACACACAGATAAAGGATACAACAGATACCAAGATGGGTAAATGCCCAAT
  TATTTTCAGTCCTCATAATCTGTACAAAGCTATGAGGCAACCAGTAGGCCATATCTCACT
  AGGCAGACTTTGACGAAGATGTTTCCCTATTTGAAACACAAGAAATTATGAAAATATGGA
  GATGAAGAGGGAGTAGTTCTGTGCTTCTTGCTGTGTTACCTTTATTATATTCCTAGTGTC
  ACTGACACTAGTTTAAAAACAGCCTGCAACAAGAATATTCTCATCACCAGCCACCTTTTC
  CACCTTTCTTCTTTTTCTGTTGCTGTTTCTTTTTTGTTGCTGGAGCCCCTGCAGGTTTCT
  GGTGTCTTGGGGGTATGATGTTACTTGTAGAGGCAGATACTGTTGCAGCACTGCCAGGTC
  TTGGGGAGGTGGGTGGGCTGCTCACAGTTTTACTGGCATCCAGTTCAGATGAAGCTCCTG
  CAGTTGCTCCCTGGGTCCCTTTTTCCATCTGATCCTTCTTTTTACCCCTCTTTCTTCCCC
  GGTAGTAAAACGTTCTCGCATTGGCAGCCATCTTTCTGGGTCTGGGGGTAACTTTGGGTC
  ATAATTCTTAGGCAATTTCCCTTCTTTTCTTTTTCCTTCTTTTCAAACTCCCTGTCCTTG
  GTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006947
• Insert Size: 2690 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006947.2, NP_008878.2
• RefSeq Size: 3852 bp
• RefSeq ORF: 2016 bp
• Locus ID: 6731
• Cytogenetics: 4q12
• Domains: TPR
• Protein Pathways: Protein export
• Gene Summary: 'This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).