ZNF205 (NM_003456) Human Tagged ORF Clone

CAT#: RC214738

ZNF205 (Myc-DDK-tagged)-Human zinc finger protein 205 (ZNF205), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418666) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214738 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ZNF205 protein (Cat# TP314738). The protein was produced from HEK293T cells transfected with ZNF205 cDNA clone (Cat# RC214738) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF205
• Synonyms: RhitH; Zfp13; ZNF210
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214738 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCAGACGGCGGAGGCATCCAGGACACCCAGGACAAGGAGACACCCCCGGAGGTTCCAGATCGTG
  GACATCCTCATCAGGAAATGCCTTCTAAGCTGGGGGAGGCGGTACCTTCAGGGGACGCTCAGGAGTCACT
  GCACATTAAGATGGAGCCCGAAGAGCCACACTCCGAGGGGGCATCGCAGGAGGATGGGGCTCAAGGTGCC
  TGGGGCTGGGCACCCCTAAGTCACGGCTCTAAGGAGAAAGCTCTCTTCCTGCCTGGCGGAGCCCTCCCCT
  CCCCCCGGATCCCCGTGCTTTCCCGAGAGGGGAGGACCAGAGACCGGCAGATGGCTGCAGCGCTCCTCAC
  TGCCTGGTCCCAGATGCCAGTGACTTTCGAGGATGTGGCCTTGTACCTCTCCCGGGAGGAGTGGGGACGG
  CTGGACCACACGCAGCAGAACTTCTACAGGGATGTCCTGCAGAAGAAAAATGGGCTGTCACTGGGCTTTC
  CCTTCAGCAGGCCTTTCTGGGCCCCTCAAGCGCACGGCAAGGGTGAGGCCTCGGGCTCCAGCCGGCAGGC
  AGGAGATGAGAAGGAGTGGAGAGGCGCGTGCACAGGAGCCGTCGAGGTGGGGCAGAGGGTGCAGACCTCA
  TCCGTGGCAGCCCTTGGGAATGTGAAGCCCTTCAGAACCAGGGCAGGGAGAGTCCAGTGGGGCGTCCCGC
  AGTGCGCGCAGGAAGCAGCCTGCGGCCGGAGCTCAGGGCCGGCCAAAGACTCCGGGCAGCCGGCTGAGCC
  AGATCGCACCCCGGATGCAGCTCCGCCAGACCCCAGTCCCACGGAGCCCCAGGAGTACCGCGTCCCGGAG
  AAGCCCAACGAGGAGGAGAAGGGCGCCCCGGAGAGTGGCGAGGAGGGCCTGGCCCCTGACAGTGAGGTGG
  GCAGGAAGAGCTACCGGTGCGAGCAGTGCGGCAAGGGCTTCAGCTGGCACTCGCACCTGGTGACGCACCG
  GCGCACGCACACGGGCGAGAAGCCCTACGCCTGCACTGACTGCGGGAAGCGCTTCGGCCGCAGCTCGCAC
  CTCATCCAGCACCAGATCATCCACACGGGCGAGAAGCCCTACACCTGCCCCGCCTGCCGGAAGAGCTTCA
  GCCACCACTCCACGCTGATTCAGCACCAGCGCATCCACACCGGAGAGAAGCCCTACGTGTGCGACCGCTG
  CGCCAAGCGCTTCACCCGCCGCTCGGACTTGGTCACCCACCAGGGCACCCACACGGGCGCCAAGCCGCAC
  AAGTGCCCCATCTGCGCCAAGTGCTTCACGCAGAGCTCGGCGCTAGTCACCCACCAGCGCACCCACACTG
  GGGTCAAGCCCTATCCGTGCCCCGAGTGCGGCAAGTGCTTCAGCCAGCGTTCCAACCTCATCGCGCACAA
  CCGCACACACACAGGCGAGAAGCCCTACCACTGCCTCGACTGCGGCAAGAGCTTCAGCCACAGCTCGCAC
  CTCACCGCGCACCAGCGCACCCACCGTGGCGTGCGGCCCTACGCCTGCCCGTTGTGCGGCAAGAGCTTCA
  GCCGGCGCTCCAACCTGCACCGGCACGAGAAGATCCACACCACCGGGCCCAAGGCCCTGGCCATGCTGAT
  GCTGGGGGCGGCGGCGGCGGGGGCTCTGGCCACACCCCCACCCGCTCCCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214738 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSADGGGIQDTQDKETPPEVPDRGHPHQEMPSKLGEAVPSGDAQESLHIKMEPEEPHSEGASQEDGAQGA
  WGWAPLSHGSKEKALFLPGGALPSPRIPVLSREGRTRDRQMAAALLTAWSQMPVTFEDVALYLSREEWGR
  LDHTQQNFYRDVLQKKNGLSLGFPFSRPFWAPQAHGKGEASGSSRQAGDEKEWRGACTGAVEVGQRVQTS
  SVAALGNVKPFRTRAGRVQWGVPQCAQEAACGRSSGPAKDSGQPAEPDRTPDAAPPDPSPTEPQEYRVPE
  KPNEEEKGAPESGEEGLAPDSEVGRKSYRCEQCGKGFSWHSHLVTHRRTHTGEKPYACTDCGKRFGRSSH
  LIQHQIIHTGEKPYTCPACRKSFSHHSTLIQHQRIHTGEKPYVCDRCAKRFTRRSDLVTHQGTHTGAKPH
  KCPICAKCFTQSSALVTHQRTHTGVKPYPCPECGKCFSQRSNLIAHNRTHTGEKPYHCLDCGKSFSHSSH
  LTAHQRTHRGVRPYACPLCGKSFSRRSNLHRHEKIHTTGPKALAMLMLGAAAAGALATPPPAPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003456
• ORF Size: 1662 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003456.2, NP_003447.2
• RefSeq Size: 2077 bp
• RefSeq ORF: 1665 bp
• Locus ID: 7755
• Protein Families: Transcription Factors
• MW: 60.6 kDa
• Gene Summary: May be involved in transcriptional regulation. [UniProtKB/Swiss-Prot Function]